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Two subsets of stem-like CD8+ memory T cell progenitors with distinct fate commitments in humans
G. Galletti, G. De Simone, EMC. Mazza, S. Puccio, C. Mezzanotte, TM. Bi, AN. Davydov, M. Metsger, E. Scamardella, G. Alvisi, F. De Paoli, V. Zanon, A. Scarpa, B. Camisa, FS. Colombo, A. Anselmo, C. Peano, S. Polletti, D. Mavilio, L. Gattinoni,...
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
C17199/A18246/A29202
Cancer Research UK - United Kingdom
100326/Z/12/Z
Wellcome Trust - United Kingdom
NLK
ProQuest Central
od 2000-07-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2000-07-01 do Před 1 rokem
Public Health Database (ProQuest)
od 2000-07-01 do Před 1 rokem
- MeSH
- biologické markery MeSH
- buněčná diferenciace imunologie MeSH
- CD8-pozitivní T-lymfocyty imunologie metabolismus MeSH
- homeostáza telomer MeSH
- imunofenotypizace MeSH
- imunologická paměť * MeSH
- lidé MeSH
- lymfoidní progenitorové buňky cytologie imunologie metabolismus MeSH
- myši MeSH
- stanovení celkové genové exprese MeSH
- T-lymfocyty - podskupiny imunologie metabolismus MeSH
- výpočetní biologie metody MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
T cell memory relies on the generation of antigen-specific progenitors with stem-like properties. However, the identity of these progenitors has remained unclear, precluding a full understanding of the differentiation trajectories that underpin the heterogeneity of antigen-experienced T cells. We used a systematic approach guided by single-cell RNA-sequencing data to map the organizational structure of the human CD8+ memory T cell pool under physiological conditions. We identified two previously unrecognized subsets of clonally, epigenetically, functionally, phenotypically and transcriptionally distinct stem-like CD8+ memory T cells. Progenitors lacking the inhibitory receptors programmed death-1 (PD-1) and T cell immunoreceptor with Ig and ITIM domains (TIGIT) were committed to a functional lineage, whereas progenitors expressing PD-1 and TIGIT were committed to a dysfunctional, exhausted-like lineage. Collectively, these data reveal the existence of parallel differentiation programs in the human CD8+ memory T cell pool, with potentially broad implications for the development of immunotherapies and vaccines.
Center for Cancer Research National Cancer Institute Bethesda MD USA
Center of Life Sciences Skolkovo Institute of Science and Technology Moscow Russia
Central European Institute of Technology Brno Czech Republic
Department of Biomedical Sciences Humanitas University Pieve Emanuele Milan Italy
Department of Medical Biotechnologies and Translational Medicine University of Milan Milan Italy
Division of Cancer and Genetics Cardiff University School of Medicine Cardiff UK
Division of Infection and Immunity Cardiff University School of Medicine Cardiff UK
Genomic Unit Humanitas Clinical and Research Center IRCCS Rozzano Milan Italy
Humanitas Flow Cytometry Core Humanitas Clinical and Research Center IRCCS Rozzano Milan Italy
Institute of Genetic and Biomedical Research UoS Milan National Research Council Rozzano Milan Italy
Pirogov Russian National Research Medical University Moscow Russia
Regensburg Center for Interventional Immunology Regensburg Germany
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry Moscow Russia
St Jude Children's Research Hospital Memphis TN USA
Systems Immunity Research Institute Cardiff University School of Medicine Cardiff UK
University of Regensburg Regensburg Germany
Vaccine and Infectious Disease Division Fred Hutchinson Cancer Research Center Seattle WA USA
Citace poskytuje Crossref.org
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