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Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)

L. Sushytskyi, P. Lukáč, A. Synytsya, R. Bleha, L. Rajsiglová, P. Capek, R. Pohl, L. Vannucci, J. Čopíková, P. Kaštánek

. 2020 ; 246 (-) : 116588. [pub] 20200612

Language English Country Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21012022

Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 × 104g mol-1) was defined mainly as branched O-2-β-xylo-(1→3)-β-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 × 105 and 9.84 × 104g mol-1) were complex polysaccharides containing α-l-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-α production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.

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$a Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 × 104g mol-1) was defined mainly as branched O-2-β-xylo-(1→3)-β-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 × 105 and 9.84 × 104g mol-1) were complex polysaccharides containing α-l-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-α production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.
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$a Lukáč, Pavol $u Czech Academy of Sciences, Institute of Microbiology, Vídeňská 1083, 142 20, Prague 4 Krč, Czech Republic; Faculty of Science, Charles University, Albertov 6, 128 00, Prague 2, Czech Republic
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$a Synytsya, Andriy $u Department of Carbohydrates and Cereals, Faculty of Food and Biochemical Technology, University of Chemistry and Technology in Prague, Technická 5, 166 28, Prague 6 Dejvice, Czech Republic
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$a Bleha, Roman $u Department of Carbohydrates and Cereals, Faculty of Food and Biochemical Technology, University of Chemistry and Technology in Prague, Technická 5, 166 28, Prague 6 Dejvice, Czech Republic
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$a Rajsiglová, Lenka $u Czech Academy of Sciences, Institute of Microbiology, Vídeňská 1083, 142 20, Prague 4 Krč, Czech Republic; Faculty of Science, Charles University, Albertov 6, 128 00, Prague 2, Czech Republic
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$a Capek, Peter $u Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38, Bratislava, Slovakia
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$a Pohl, Radek $u Institute of Organic Chemistry and Biochemistry AS CR, Flemingovo sq. 2, 166 28, Prague 6, Czech Republic
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$a Vannucci, Luca $u Czech Academy of Sciences, Institute of Microbiology, Vídeňská 1083, 142 20, Prague 4 Krč, Czech Republic
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$a Čopíková, Jana $u Department of Carbohydrates and Cereals, Faculty of Food and Biochemical Technology, University of Chemistry and Technology in Prague, Technická 5, 166 28, Prague 6 Dejvice, Czech Republic
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$a Kaštánek, Petr $u Department of Biotechnology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology in Prague, Technická 5, 166 28, Prague 6 Dejvice, Czech Republic; EcoFuel Laboratories s.r.o., Ocelářská 9, Prague 9 Libeň, 190 00, Czech Republic
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