Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Light sheet fluorescence microscopy of optically cleared brains for studying the glymphatic system

NB. Bèchet, TM. Kylkilahti, B. Mattsson, M. Petrasova, NC. Shanbhag, I. Lundgaard

. 2020 ; 40 (10) : 1975-1986. [pub] 20200611

Language English Country United States

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc21012081

Fluid transport in the perivascular space by the glia-lymphatic (glymphatic) system is important for the removal of solutes from the brain parenchyma, including peptides such as amyloid-beta which are implicated in the pathogenesis of Alzheimer's disease. The glymphatic system is highly active in the sleep state and under the influence of certain of anaesthetics, while it is suppressed in the awake state and by other anaesthetics. Here we investigated whether light sheet fluorescence microscopy of whole optically cleared murine brains was capable of detecting glymphatic differences in sleep- and awake-mimicking anaesthesia, respectively. Using light-sheet imaging of whole brains, we found anaesthetic-dependent cerebrospinal fluid (CSF) influx differences, including reduced tracer influx along tertiary branches of the middle cerebral artery and reduced influx along dorsal and anterior penetrating arterioles, in the awake-mimicking anaesthesia. This study establishes that light sheet microscopy of optically cleared brains is feasible for quantitative analyses and can provide images of the entire glymphatic system in whole brains.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21012081
003      
CZ-PrNML
005      
20210713112105.0
007      
ta
008      
210420s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1177/0271678X20924954 $2 doi
035    __
$a (PubMed)32525440
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Bèchet, Nicholas B $u Department of Experimental Medical Science, Lund University, Lund, Sweden ; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden
245    10
$a Light sheet fluorescence microscopy of optically cleared brains for studying the glymphatic system / $c NB. Bèchet, TM. Kylkilahti, B. Mattsson, M. Petrasova, NC. Shanbhag, I. Lundgaard
520    9_
$a Fluid transport in the perivascular space by the glia-lymphatic (glymphatic) system is important for the removal of solutes from the brain parenchyma, including peptides such as amyloid-beta which are implicated in the pathogenesis of Alzheimer's disease. The glymphatic system is highly active in the sleep state and under the influence of certain of anaesthetics, while it is suppressed in the awake state and by other anaesthetics. Here we investigated whether light sheet fluorescence microscopy of whole optically cleared murine brains was capable of detecting glymphatic differences in sleep- and awake-mimicking anaesthesia, respectively. Using light-sheet imaging of whole brains, we found anaesthetic-dependent cerebrospinal fluid (CSF) influx differences, including reduced tracer influx along tertiary branches of the middle cerebral artery and reduced influx along dorsal and anterior penetrating arterioles, in the awake-mimicking anaesthesia. This study establishes that light sheet microscopy of optically cleared brains is feasible for quantitative analyses and can provide images of the entire glymphatic system in whole brains.
650    _2
$a anestezie $7 D000758
650    _2
$a zvířata $7 D000818
650    _2
$a arterioly $x fyziologie $7 D001160
650    _2
$a mozek $x ultrastruktura $7 D001921
650    _2
$a mozkomíšní mok $x metabolismus $7 D002555
650    _2
$a mozkový krevní oběh $x fyziologie $7 D002560
650    _2
$a glymfatický systém $x fyziologie $7 D000077502
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a myši $7 D051379
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a fluorescenční mikroskopie $x metody $7 D008856
650    _2
$a arteria cerebri media $x fyziologie $7 D020768
650    _2
$a neurozobrazování $x metody $7 D059906
650    _2
$a spánek $x fyziologie $7 D012890
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kylkilahti, Tekla M $u Department of Experimental Medical Science, Lund University, Lund, Sweden ; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden
700    1_
$a Mattsson, Bengt $u Developmental and Regenerative Neurobiology, Wallenberg Neuroscience Center, Lund University, Lund, Sweden
700    1_
$a Petrasova, Martina $u Department of Experimental Medical Science, Lund University, Lund, Sweden ; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden ; Department of Neurology, University Hospital Brno, Brno, Czech Republic
700    1_
$a Shanbhag, Nagesh C $u Department of Experimental Medical Science, Lund University, Lund, Sweden ; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden
700    1_
$a Lundgaard, Iben $u Department of Experimental Medical Science, Lund University, Lund, Sweden ; Wallenberg Centre for Molecular Medicine, Lund University, Lund, Sweden
773    0_
$w MED00009982 $t Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism $x 1559-7016 $g Roč. 40, č. 10 (2020), s. 1975-1986
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32525440 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210420 $b ABA008
991    __
$a 20210713112102 $b ABA008
999    __
$a ok $b bmc $g 1650460 $s 1132460
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 40 $c 10 $d 1975-1986 $e 20200611 $i 1559-7016 $m Journal of cerebral blood flow and metabolism $n J Cereb Blood Flow Metab $x MED00009982
LZP    __
$a Pubmed-20210420
Back

Find record

Citation metrics

Archiving options