Detail
Článek
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress

T. van der Meer, A. Verlee, P. Willems, F. Impens, K. Gevaert, C. Testerink, CV. Stevens, F. Van Breusegem, P. Kerchev

. 2020 ; 9 (9) : . [pub] 20200902

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21012163

Alterations of hydrogen peroxide (H2O2) levels have a profound impact on numerous signaling cascades orchestrating plant growth, development, and stress signaling, including programmed cell death. To expand the repertoire of known molecular mechanisms implicated in H2O2 signaling, we performed a forward chemical screen to identify small molecules that could alleviate the photorespiratory-induced cell death phenotype of Arabidopsisthaliana mutants lacking H2O2-scavenging capacity by peroxisomal catalase2. Here, we report the characterization of pakerine, an m-sulfamoyl benzamide from the sulfonamide family. Pakerine alleviates the cell death phenotype of cat2 mutants exposed to photorespiration-promoting conditions and delays dark-induced senescence in wild-type Arabidopsis leaves. By using a combination of transcriptomics, metabolomics, and affinity purification, we identified abnormal inflorescence meristem 1 (AIM1) as a putative protein target of pakerine. AIM1 is a 3-hydroxyacyl-CoA dehydrogenase involved in fatty acid β-oxidation that contributes to jasmonic acid (JA) and salicylic acid (SA) biosynthesis. Whereas intact JA biosynthesis was not required for pakerine bioactivity, our results point toward a role for β-oxidation-dependent SA production in the execution of H2O2-mediated cell death.

000      
00000naa a2200000 a 4500
001      
bmc21012163
003      
CZ-PrNML
005      
20210507101925.0
007      
ta
008      
210420s2020 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/cells9092026 $2 doi
035    __
$a (PubMed)32887516
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a van der Meer, Tom $u VIB Center for Plant Systems Biology, B-9052 Ghent, Belgium $u Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium
245    10
$a Chemical Genetics Approach Identifies Abnormal Inflorescence Meristem 1 as a Putative Target of a Novel Sulfonamide That Protects Catalase2-Deficient Arabidopsis against Photorespiratory Stress / $c T. van der Meer, A. Verlee, P. Willems, F. Impens, K. Gevaert, C. Testerink, CV. Stevens, F. Van Breusegem, P. Kerchev
520    9_
$a Alterations of hydrogen peroxide (H2O2) levels have a profound impact on numerous signaling cascades orchestrating plant growth, development, and stress signaling, including programmed cell death. To expand the repertoire of known molecular mechanisms implicated in H2O2 signaling, we performed a forward chemical screen to identify small molecules that could alleviate the photorespiratory-induced cell death phenotype of Arabidopsisthaliana mutants lacking H2O2-scavenging capacity by peroxisomal catalase2. Here, we report the characterization of pakerine, an m-sulfamoyl benzamide from the sulfonamide family. Pakerine alleviates the cell death phenotype of cat2 mutants exposed to photorespiration-promoting conditions and delays dark-induced senescence in wild-type Arabidopsis leaves. By using a combination of transcriptomics, metabolomics, and affinity purification, we identified abnormal inflorescence meristem 1 (AIM1) as a putative protein target of pakerine. AIM1 is a 3-hydroxyacyl-CoA dehydrogenase involved in fatty acid β-oxidation that contributes to jasmonic acid (JA) and salicylic acid (SA) biosynthesis. Whereas intact JA biosynthesis was not required for pakerine bioactivity, our results point toward a role for β-oxidation-dependent SA production in the execution of H2O2-mediated cell death.
650    _2
$a Arabidopsis $x cytologie $x účinky léků $x genetika $x metabolismus $7 D017360
650    _2
$a proteiny huseníčku $x genetika $x metabolismus $7 D029681
650    _2
$a buněčná smrt $x účinky léků $7 D016923
650    _2
$a buněčné dýchání $x účinky léků $x genetika $7 D019069
650    _2
$a výpočetní biologie $x metody $7 D019295
650    _2
$a cyklopentany $x metabolismus $7 D003517
650    _2
$a stanovení celkové genové exprese $7 D020869
650    12
$a regulace genové exprese u rostlin $7 D018506
650    _2
$a peroxid vodíku $x antagonisté a inhibitory $x farmakologie $7 D006861
650    _2
$a hydroponie $x metody $7 D018527
650    _2
$a meristém $x cytologie $x účinky léků $x metabolismus $7 D018519
650    _2
$a multienzymové komplexy $x genetika $x metabolismus $7 D009097
650    _2
$a oxylipiny $x metabolismus $7 D054883
650    _2
$a fotosyntéza $x účinky léků $x genetika $7 D010788
650    _2
$a rostlinné buňky $x účinky léků $x metabolismus $7 D059828
650    _2
$a listy rostlin $x cytologie $x účinky léků $x metabolismus $7 D018515
650    _2
$a kyselina salicylová $x metabolismus $7 D020156
650    _2
$a semena rostlinná $x účinky léků $7 D012639
650    _2
$a signální transdukce $7 D015398
650    _2
$a fyziologický stres $7 D013312
650    _2
$a sulfonamidy $x chemická syntéza $x farmakologie $7 D013449
650    _2
$a transkriptom $7 D059467
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Verlee, Arno $u Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, B-9000 Ghent, Belgium
700    1_
$a Willems, Patrick $u VIB Center for Plant Systems Biology, B-9052 Ghent, Belgium $u Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium
700    1_
$a Impens, Francis $u VIB Proteomics Core, B-9000 Ghent, Belgium $u Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, B-9000 Ghent, Belgium $u VIB Center for Medical Biotechnology, B-9052 Ghent, Belgium
700    1_
$a Gevaert, Kris $u Department of Biomolecular Medicine, Faculty of Medicine and Health Sciences, Ghent University, B-9000 Ghent, Belgium $u VIB Center for Medical Biotechnology, B-9052 Ghent, Belgium
700    1_
$a Testerink, Christa $u Laboratory of Plant Physiology, Plant Sciences Group, Wageningen University and Research, 6708 PB Wageningen, The Netherlands
700    1_
$a Stevens, Christian V $u Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, B-9000 Ghent, Belgium
700    1_
$a Van Breusegem, Frank $u VIB Center for Plant Systems Biology, B-9052 Ghent, Belgium $u Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium
700    1_
$a Kerchev, Pavel $u VIB Center for Plant Systems Biology, B-9052 Ghent, Belgium $u Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium $u Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech Republic $u Phytophthora Research Centre, Mendel University in Brno, 61300 Brno, Czech Republic
773    0_
$w MED00194911 $t Cells $x 2073-4409 $g Roč. 9, č. 9 (2020)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32887516 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210420 $b ABA008
991    __
$a 20210507101925 $b ABA008
999    __
$a ok $b bmc $g 1650522 $s 1132542
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 9 $c 9 $e 20200902 $i 2073-4409 $m Cells $n Cells $x MED00194911
LZP    __
$a Pubmed-20210420

Najít záznam

Citační ukazatele

Možnosti archivace