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Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC
P. Kozlik, K. Molnarova, T. Jecmen, T. Krizek, R. Goldman
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
Grantová podpora
R01 CA135069
NCI NIH HHS - United States
R01 CA238455
NCI NIH HHS - United States
S10 OD023557
NIH HHS - United States
U01 CA230692
NCI NIH HHS - United States
- MeSH
- acetonitrily chemie MeSH
- glykopeptidy analýza chemie izolace a purifikace MeSH
- hydrofobní a hydrofilní interakce MeSH
- kyselina N-acetylneuraminová chemie MeSH
- polysacharidy chemie MeSH
- rozpouštědla chemie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Publikační typ
- časopisecké články MeSH
The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC). In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins. The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan. We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid. Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.
Citace poskytuje Crossref.org
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- $a Kozlik, Petr $u Department of Analytical Chemistry, Faculty of Science, Charles University, Hlavova 8, 128 43 Prague 2, Czech Republic. Electronic address: kozlik@natur.cuni.cz
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- $a The composition of a sample solvent has a crucial impact on separations in hydrophilic interaction liquid chromatography (HILIC). In this short communication, we studied the effect of an organic modifier in the sample solvent on the solubility of different tryptic glycopeptides of hemopexin and haptoglobin proteins. The results showed that the solubility of glycopeptides in solvents with a high acetonitrile content depends on the type of attached N-glycan. We observed lower solubility in larger glycans attached to the same peptide backbone, and we demonstrated that glycopeptides containing sialic acids precipitate more readily than those without sialic acid. Therefore, the sample solvent composition in HILIC must be carefully optimized for accurate quantitative data collection and for adequate separation.
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- $a Molnarova, Katarina $u Department of Analytical Chemistry, Faculty of Science, Charles University, Hlavova 8, 128 43 Prague 2, Czech Republic
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