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A device for investigation of natural cell mobility and deformability
V. Ledvina, K. Klepárník, S. Legartová, E. Bártová
Language English Country Germany
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
Strategy program (Qualitas)
Akademie Věd České Republiky - International
17-01995s
Grantová Agentura České Republiky - International
- MeSH
- Cell Culture Techniques instrumentation MeSH
- Equipment Design MeSH
- Dimethylpolysiloxanes chemistry MeSH
- HeLa Cells MeSH
- Microscopy, Confocal MeSH
- Humans MeSH
- Microfluidic Analytical Techniques instrumentation MeSH
- Cell Movement physiology MeSH
- Cell Shape physiology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
A microfluidic device made of polydimethylsiloxane was developed for continuous evaluation of natural migration mobility of many eukaryotic cells in relaxed and deformed state. The device was fabricated by standard photolithography and soft lithography techniques using the SU-8 3010 negative photoresist on a glass wafer as the master mold. The simple flow-free device exploits the chemotactic movement of cells through a set of mechanical barriers in the direction of concentration gradients of attractants. The barriers are formed by arrays of circular cross-section pillars with decreasing spacing 7, 5, and 3 μm. To pass through the obstacles, the cells are deformed and change their cytoskeletal architecture. The instantaneous migration velocities of cells are monitored in a time-lapse setup of the scanning confocal microscope. Thus, the cellular deformability and migratory activity can easily be evaluated. The functionality of the device was tested with model HeLa cells stably transfected with fluorescent Premo FUCCI Cell Cycle Sensor. The designed device has the potential to be implemented for testing the tendency of patients' tumors to metastasis.
Faculty of Science Masaryk University Brno Czech Republic
Institute of Analytical Chemistry of the Czech Academy of Sciences Brno Czech Republic
Institute of the Biophysics of the Czech Academy of Sciences Brno Czech Republic
References provided by Crossref.org
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