-
Je něco špatně v tomto záznamu ?
Mapping the Gene Expression Spectrum of Mediator Subunits in Response to Viroid Infection in Plants
VS. Nath, A. Shrestha, P. Awasthi, AK. Mishra, T. Kocábek, J. Matoušek, A. Sečnik, J. Jakše, S. Radišek, V. Hallan
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Grantová podpora
CZ.02.2.69/0.0/0.0/16_027/0008357
MEMOBIC (EU Operational Programme Research, Development and Education)
P4-0077
ARRS research programme
RVO:60077344
IPMB institutional support
Free Medical Journals od 2000
Freely Accessible Science Journals od 2000
PubMed Central od 2007
Europe PubMed Central od 2007
ProQuest Central od 2000-03-01
Open Access Digital Library od 2000-01-01
Open Access Digital Library od 2007-01-01
Health & Medicine (ProQuest) od 2000-03-01
ROAD: Directory of Open Access Scholarly Resources od 2000
Odkazy
PubMed
32260277
DOI
10.3390/ijms21072498
Knihovny.cz E-zdroje
- MeSH
- druhová specificita MeSH
- Humulus genetika virologie MeSH
- listy rostlin genetika mikrobiologie MeSH
- mediátorový komplex genetika MeSH
- regulace genové exprese u rostlin MeSH
- rostlinné proteiny genetika MeSH
- rostlinné viry MeSH
- stanovení celkové genové exprese MeSH
- tabák genetika virologie MeSH
- viroidy genetika patogenita MeSH
- Publikační typ
- časopisecké články MeSH
The mediator (MED) represents a large, conserved, multi-subunit protein complex that regulates gene expression through interactions with RNA polymerase II and enhancer-bound transcription factors. Expanding research accomplishments suggest the predominant role of plant MED subunits in the regulation of various physiological and developmental processes, including the biotic stress response against bacterial and fungal pathogens. However, the involvement of MED subunits in virus/viroid pathogenesis remains elusive. In this study, we investigated for the first time the gene expression modulation of selected MED subunits in response to five viroid species (Apple fruit crinkle viroid (AFCVd), Citrus bark cracking viroid (CBCVd), Hop latent viroid (HLVd), Hop stunt viroid (HSVd), and Potato spindle tuber viroid (PSTVd)) in two model plant species (Nicotiana tabacum and N. benthamiana) and a commercially important hop (Humulus lupulus) cultivar. Our results showed a differential expression pattern of MED subunits in response to a viroid infection. The individual plant MED subunits displayed a differential and tailored expression pattern in response to different viroid species, suggesting that the MED expression is viroid- and plant species-dependent. The explicit evidence obtained from our results warrants further investigation into the association of the MED subunit with symptom development. Together, we provide a comprehensive portrait of MED subunit expression in response to viroid infection and a plausible involvement of MED subunits in fine-tuning transcriptional reprogramming in response to viroid infection, suggesting them as a potential candidate for rewiring the defense response network in plants against pathogens.
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21012719
- 003
- CZ-PrNML
- 005
- 20210507102526.0
- 007
- ta
- 008
- 210420s2020 sz f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.3390/ijms21072498 $2 doi
- 035 __
- $a (PubMed)32260277
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Nath, Vishnu Sukumari $u Biology Centre of the Czech Academy of Sciences, Department of Molecular Genetics, Institute of Plant Molecular Biology, Branišovská 31, 370 05 Ceske Budejovice, Czech Republic
- 245 10
- $a Mapping the Gene Expression Spectrum of Mediator Subunits in Response to Viroid Infection in Plants / $c VS. Nath, A. Shrestha, P. Awasthi, AK. Mishra, T. Kocábek, J. Matoušek, A. Sečnik, J. Jakše, S. Radišek, V. Hallan
- 520 9_
- $a The mediator (MED) represents a large, conserved, multi-subunit protein complex that regulates gene expression through interactions with RNA polymerase II and enhancer-bound transcription factors. Expanding research accomplishments suggest the predominant role of plant MED subunits in the regulation of various physiological and developmental processes, including the biotic stress response against bacterial and fungal pathogens. However, the involvement of MED subunits in virus/viroid pathogenesis remains elusive. In this study, we investigated for the first time the gene expression modulation of selected MED subunits in response to five viroid species (Apple fruit crinkle viroid (AFCVd), Citrus bark cracking viroid (CBCVd), Hop latent viroid (HLVd), Hop stunt viroid (HSVd), and Potato spindle tuber viroid (PSTVd)) in two model plant species (Nicotiana tabacum and N. benthamiana) and a commercially important hop (Humulus lupulus) cultivar. Our results showed a differential expression pattern of MED subunits in response to a viroid infection. The individual plant MED subunits displayed a differential and tailored expression pattern in response to different viroid species, suggesting that the MED expression is viroid- and plant species-dependent. The explicit evidence obtained from our results warrants further investigation into the association of the MED subunit with symptom development. Together, we provide a comprehensive portrait of MED subunit expression in response to viroid infection and a plausible involvement of MED subunits in fine-tuning transcriptional reprogramming in response to viroid infection, suggesting them as a potential candidate for rewiring the defense response network in plants against pathogens.
- 650 _2
- $a stanovení celkové genové exprese $7 D020869
- 650 _2
- $a regulace genové exprese u rostlin $7 D018506
- 650 _2
- $a Humulus $x genetika $x virologie $7 D027582
- 650 _2
- $a mediátorový komplex $x genetika $7 D056892
- 650 _2
- $a listy rostlin $x genetika $x mikrobiologie $7 D018515
- 650 _2
- $a rostlinné proteiny $x genetika $7 D010940
- 650 _2
- $a rostlinné viry $7 D010942
- 650 _2
- $a druhová specificita $7 D013045
- 650 _2
- $a tabák $x genetika $x virologie $7 D014026
- 650 _2
- $a viroidy $x genetika $x patogenita $7 D014772
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Shrestha, Ankita $u Biology Centre of the Czech Academy of Sciences, Department of Molecular Genetics, Institute of Plant Molecular Biology, Branišovská 31, 370 05 Ceske Budejovice, Czech Republic
- 700 1_
- $a Awasthi, Praveen $u Biology Centre of the Czech Academy of Sciences, Department of Molecular Genetics, Institute of Plant Molecular Biology, Branišovská 31, 370 05 Ceske Budejovice, Czech Republic
- 700 1_
- $a Mishra, Ajay Kumar $u Biology Centre of the Czech Academy of Sciences, Department of Molecular Genetics, Institute of Plant Molecular Biology, Branišovská 31, 370 05 Ceske Budejovice, Czech Republic
- 700 1_
- $a Kocábek, Tomáš $u Biology Centre of the Czech Academy of Sciences, Department of Molecular Genetics, Institute of Plant Molecular Biology, Branišovská 31, 370 05 Ceske Budejovice, Czech Republic
- 700 1_
- $a Matoušek, Jaroslav $u Biology Centre of the Czech Academy of Sciences, Department of Molecular Genetics, Institute of Plant Molecular Biology, Branišovská 31, 370 05 Ceske Budejovice, Czech Republic
- 700 1_
- $a Sečnik, Andrej $u Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
- 700 1_
- $a Jakše, Jernej $u Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia
- 700 1_
- $a Radišek, Sebastjan $u Slovenian Institute of Hop Research and Brewing, Plant Protection Department, Cesta Žalskega Tabora 2, SI-3310 Žalec, Slovenia
- 700 1_
- $a Hallan, Vipin $u CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061 India
- 773 0_
- $w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 21, č. 7 (2020)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/32260277 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20210420 $b ABA008
- 991 __
- $a 20210507102525 $b ABA008
- 999 __
- $a ok $b bmc $g 1650975 $s 1133098
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 21 $c 7 $e 20200403 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
- GRA __
- $a CZ.02.2.69/0.0/0.0/16_027/0008357 $p MEMOBIC (EU Operational Programme Research, Development and Education)
- GRA __
- $a P4-0077 $p ARRS research programme
- GRA __
- $a RVO:60077344 $p IPMB institutional support
- LZP __
- $a Pubmed-20210420