• Something wrong with this record ?

The Role of miR-21 in Osteoblasts-Osteoclasts Coupling In Vitro

A. Smieszek, K. Marcinkowska, A. Pielok, M. Sikora, L. Valihrach, K. Marycz

. 2020 ; 9 (2) : . [pub] 20200219

Language English Country Switzerland

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc21012854

MiR-21 is being gradually more and more recognized as a molecule regulating bone tissue homeostasis. However, its function is not fully understood due to the dual role of miR-21 on bone-forming and bone-resorbing cells. In this study, we investigated the impact of miR-21 inhibition on pre-osteoblastic cells differentiation and paracrine signaling towards pre-osteoclasts using indirect co-culture model of mouse pre-osteoblast (MC3T3) and pre-osteoclast (4B12) cell lines. The inhibition of miR-21 in MC3T3 cells (MC3T3inh21) modulated expression of genes encoding osteogenic markers including collagen type I (Coll-1), osteocalcin (Ocl), osteopontin (Opn), and runt-related transcription factor 2 (Runx-2). Inhibition of miR-21 in osteogenic cultures of MC3T3 also inflected the synthesis of OPN protein which is essential for proper mineralization of extracellular matrix (ECM) and anchoring osteoclasts to the bones. Furthermore, it was shown that in osteoblasts miR-21 regulates expression of factors that are vital for survival of pre-osteoclast, such as receptor activator of nuclear factor κB ligand (RANKL). The pre-osteoclast cultured with MC3T3inh21 cells was characterized by lowered expression of several markers associated with osteoclasts' differentiation, foremost tartrate-resistant acid phosphatase (Trap) but also receptor activator of nuclear factor-κB ligand (Rank), cathepsin K (Ctsk), carbonic anhydrase II (CaII), and matrix metalloproteinase (Mmp-9). Collectively, our data indicate that the inhibition of miR-21 in MC3T3 cells impairs the differentiation and ECM mineralization as well as influences paracrine signaling leading to decreased viability of pre-osteoclasts.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21012854
003      
CZ-PrNML
005      
20210507101904.0
007      
ta
008      
210420s2020 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/cells9020479 $2 doi
035    __
$a (PubMed)32093031
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Smieszek, Agnieszka $u Department of Experimental Biology, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, 50-375 Wroclaw, Poland
245    14
$a The Role of miR-21 in Osteoblasts-Osteoclasts Coupling In Vitro / $c A. Smieszek, K. Marcinkowska, A. Pielok, M. Sikora, L. Valihrach, K. Marycz
520    9_
$a MiR-21 is being gradually more and more recognized as a molecule regulating bone tissue homeostasis. However, its function is not fully understood due to the dual role of miR-21 on bone-forming and bone-resorbing cells. In this study, we investigated the impact of miR-21 inhibition on pre-osteoblastic cells differentiation and paracrine signaling towards pre-osteoclasts using indirect co-culture model of mouse pre-osteoblast (MC3T3) and pre-osteoclast (4B12) cell lines. The inhibition of miR-21 in MC3T3 cells (MC3T3inh21) modulated expression of genes encoding osteogenic markers including collagen type I (Coll-1), osteocalcin (Ocl), osteopontin (Opn), and runt-related transcription factor 2 (Runx-2). Inhibition of miR-21 in osteogenic cultures of MC3T3 also inflected the synthesis of OPN protein which is essential for proper mineralization of extracellular matrix (ECM) and anchoring osteoclasts to the bones. Furthermore, it was shown that in osteoblasts miR-21 regulates expression of factors that are vital for survival of pre-osteoclast, such as receptor activator of nuclear factor κB ligand (RANKL). The pre-osteoclast cultured with MC3T3inh21 cells was characterized by lowered expression of several markers associated with osteoclasts' differentiation, foremost tartrate-resistant acid phosphatase (Trap) but also receptor activator of nuclear factor-κB ligand (Rank), cathepsin K (Ctsk), carbonic anhydrase II (CaII), and matrix metalloproteinase (Mmp-9). Collectively, our data indicate that the inhibition of miR-21 in MC3T3 cells impairs the differentiation and ECM mineralization as well as influences paracrine signaling leading to decreased viability of pre-osteoclasts.
650    _2
$a zvířata $7 D000818
650    _2
$a resorpce kosti $x metabolismus $7 D001862
650    _2
$a buněčná diferenciace $x genetika $7 D002454
650    _2
$a buněčné linie $7 D002460
650    _2
$a kokultivační techniky $7 D018920
650    _2
$a protein PEBP2alfaA $x genetika $x metabolismus $7 D050659
650    _2
$a extracelulární matrix $x metabolismus $7 D005109
650    _2
$a myši $7 D051379
650    _2
$a mikro RNA $x genetika $x metabolismus $7 D035683
650    _2
$a osteoblasty $x metabolismus $7 D010006
650    _2
$a osteoklasty $x metabolismus $7 D010010
650    _2
$a osteogeneze $x genetika $7 D010012
650    _2
$a osteopontin $x genetika $x metabolismus $7 D053495
650    _2
$a parakrinní signalizace $x genetika $7 D019899
650    _2
$a messenger RNA $x genetika $7 D012333
650    _2
$a signální transdukce $x genetika $7 D015398
650    _2
$a kyselá fosfatasa rezistentní k tartarátu $x metabolismus $7 D000071681
650    _2
$a transfekce $7 D014162
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Marcinkowska, Klaudia $u Department of Experimental Biology, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, 50-375 Wroclaw, Poland
700    1_
$a Pielok, Ariadna $u Department of Experimental Biology, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, 50-375 Wroclaw, Poland
700    1_
$a Sikora, Mateusz $u Department of Experimental Biology, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, 50-375 Wroclaw, Poland
700    1_
$a Valihrach, Lukas $u Laboratory of Gene Expression, Institute of Biotechnology CAS, Biocev, 25250 Vestec, Czech Republic
700    1_
$a Marycz, Krzysztof $u Department of Experimental Biology, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences, 50-375 Wroclaw, Poland $u International Institute of Translational Medicine, Jesionowa 11 St, 55-124 Malin, Poland $u Collegium Medicum, Cardinal Stefan Wyszyński University (UKSW), Woycickiego 1/3, 01-938 Warsaw, Poland
773    0_
$w MED00194911 $t Cells $x 2073-4409 $g Roč. 9, č. 2 (2020)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32093031 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210420 $b ABA008
991    __
$a 20210507101904 $b ABA008
999    __
$a ok $b bmc $g 1651098 $s 1133233
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 9 $c 2 $e 20200219 $i 2073-4409 $m Cells $n Cells $x MED00194911
LZP    __
$a Pubmed-20210420

Find record

Citation metrics

Archiving options