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CD4+ T Follicular Helper Cells in Human Tonsils and Blood Are Clonally Convergent but Divergent from Non-Tfh CD4+ Cells

E. Brenna, AN. Davydov, K. Ladell, JE. McLaren, P. Bonaiuti, M. Metsger, JD. Ramsden, SC. Gilbert, T. Lambe, DA. Price, SL. Campion, DM. Chudakov, P. Borrow, AJ. McMichael

. 2020 ; 30 (1) : 137-152.e5. [pub] 20200107

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21012924

Grantová podpora
MR/K012037 Medical Research Council - United Kingdom
MR/K012037/1 Medical Research Council - United Kingdom
100326/Z/12/Z Wellcome Trust - United Kingdom
Z01 AI000645 Intramural NIH HHS - United States
Wellcome Trust - United Kingdom
UM1 AI144371 NIAID NIH HHS - United States

T follicular helper (Tfh) cells are fundamental for B cell selection and antibody maturation in germinal centers. Circulating Tfh (cTfh) cells constitute a minor proportion of the CD4+ T cells in peripheral blood, but their clonotypic relationship to Tfh populations resident in lymph nodes and the extent to which they differ from non-Tfh CD4+ cells have been unclear. Using donor-matched blood and tonsil samples, we investigate T cell receptor (TCR) sharing between tonsillar Tfh cells and peripheral Tfh and non-Tfh cell populations. TCR transcript sequencing reveals considerable clonal overlap between peripheral and tonsillar Tfh cell subsets as well as a clear distinction between Tfh and non-Tfh cells. Furthermore, influenza-specific cTfh cell clones derived from blood can be found in the repertoire of tonsillar Tfh cells. Therefore, human blood samples can be used to gain insight into the specificity of Tfh responses occurring in lymphoid tissues, provided that cTfh subsets are studied.

Citace poskytuje Crossref.org

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