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Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood

P. Holicek, I. Truxova, L. Kasikova, S. Vosahlikova, C. Salek, J. Rakova, M. Holubova, D. Lysak, I. Cremer, R. Spisek, J. Fucikova

. 2020 ; 631 (-) : 277-287. [pub] 20190627

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21012971

Natural killer (NK) cells constitute the predominant innate lymphocyte subset that mediates the anti-viral and anti-tumor immune responses. NK cells use an array of innate receptors to sense their environment and to respond to infections, cellular stress and transformation. The resulting NK cell activation, including cytotoxicity and cytokine production, is a fundamental component of the early immune response. The most recent discoveries in NK cell biology have stimulated the translational research that has led to remarkable results for the treatment of human malignancies. Therefore, the rapid isolation of NK cells from the peripheral blood or tumor microenvironment and the subsequent assessment of cytolytic function are crucial to the study of their potency and NK cell-mediated immunosurveillance. Here, we provide protocols for NK cell isolation and the assessment of NK cell cytotoxicity using flow cytometry.

Citace poskytuje Crossref.org

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$a Natural killer (NK) cells constitute the predominant innate lymphocyte subset that mediates the anti-viral and anti-tumor immune responses. NK cells use an array of innate receptors to sense their environment and to respond to infections, cellular stress and transformation. The resulting NK cell activation, including cytotoxicity and cytokine production, is a fundamental component of the early immune response. The most recent discoveries in NK cell biology have stimulated the translational research that has led to remarkable results for the treatment of human malignancies. Therefore, the rapid isolation of NK cells from the peripheral blood or tumor microenvironment and the subsequent assessment of cytolytic function are crucial to the study of their potency and NK cell-mediated immunosurveillance. Here, we provide protocols for NK cell isolation and the assessment of NK cell cytotoxicity using flow cytometry.
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$a Truxova, Iva $u Sotio, Prague, Czech Republic
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$a Kasikova, Lenka $u Department of Immunology, Charles University, 2nd Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic
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$a Vosahlikova, Sarka $u Sotio, Prague, Czech Republic
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$a Salek, Cyril $u Institute of Hematology and Blood Transfusion, Prague, Czech Republic; Institute of Clinical and Experimental Hematology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
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$a Rakova, Jana $u Sotio, Prague, Czech Republic
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$a Holubova, Monika $u Biomedical Center, Medical Faculty in Pilsen, Charles University, Pilsen, Czech Republic
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$a Lysak, Daniel $u Department of Hematology and Oncology, Hospital in Pilsen, Pilsen, Czech Republic
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$a Cremer, Isabelle $u Team Inflammation, Complement and Cancer, INSERM, U1138, Centre de Recherche des Cordeliers, Paris, France; Sorbonne Universite de Paris, Paris, France
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$a Spisek, Radek $u Department of Immunology, Charles University, 2nd Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic
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$a Fucikova, Jitka $u Department of Immunology, Charles University, 2nd Faculty of Medicine and University Hospital Motol, Prague, Czech Republic; Sotio, Prague, Czech Republic. Electronic address: fucikova@sotio.com
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