Proteins MiaA and MiaB catalyze sequential isopentenylation and methylthiolation, respectively, of adenosine residue in 37th position of tRNAXXA. The mia mutations were recently shown by us to affect secondary metabolism and morphology of Streptomyces. However, it remained unknown as to whether both or one of the aforementioned modifications is critical for colony development and antibiotic production. Here, we addressed this issue through analysis of Streptomyces albus J1074 strains carrying double miaAmiaB knockout or extra copy of miaB gene. The double mutant differed from wild-type and miaA-minus strains in severity of morphological defects, growth dynamics, and secondary metabolism. Introduction of extra copy of miaB gene into miaA mutant restored aerial mycelium formation to the latter on certain solid media. Hence, miaB gene might be involved in tRNA thiomethylation in the absence of miaA; either MiaA- or MiaB-mediated modification appears to be enough to support normal metabolic and morphological processes in Streptomyces.

Department of Genetics and Biotechnology Ivan Franko National University of Lviv 4 Hrushevskoho st Lviv 79005 Ukraine

Department of Physics of Earth Ivan Franko National University of Lviv 4 Hrushevskoho st Lviv 79005 Ukraine

Institut für Chemie Technische Universität Berlin Straβe des 17 Juni 124 TC2 10623 Berlin Germany

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$a Koshla, Oksana $u Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 4 Hrushevskoho st, Lviv, 79005, Ukraine

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$a Genetic analysis of Streptomyces albus J1074 mia mutants suggests complex relationships between post-transcriptional tRNAXXA modifications and physiological traits / $c O. Koshla, V. Kravets, Y. Dacyuk, I. Ostash, R. Süssmuth, B. Ostash

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$a Proteins MiaA and MiaB catalyze sequential isopentenylation and methylthiolation, respectively, of adenosine residue in 37th position of tRNAXXA. The mia mutations were recently shown by us to affect secondary metabolism and morphology of Streptomyces. However, it remained unknown as to whether both or one of the aforementioned modifications is critical for colony development and antibiotic production. Here, we addressed this issue through analysis of Streptomyces albus J1074 strains carrying double miaAmiaB knockout or extra copy of miaB gene. The double mutant differed from wild-type and miaA-minus strains in severity of morphological defects, growth dynamics, and secondary metabolism. Introduction of extra copy of miaB gene into miaA mutant restored aerial mycelium formation to the latter on certain solid media. Hence, miaB gene might be involved in tRNA thiomethylation in the absence of miaA; either MiaA- or MiaB-mediated modification appears to be enough to support normal metabolic and morphological processes in Streptomyces.

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$a Kravets, Volodymyr $u Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 4 Hrushevskoho st, Lviv, 79005, Ukraine

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$a Dacyuk, Yuriy $u Department of Physics of Earth, Ivan Franko National University of Lviv, 4 Hrushevskoho st, Lviv, 79005, Ukraine

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$a Ostash, Iryna $u Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 4 Hrushevskoho st, Lviv, 79005, Ukraine

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$a Süssmuth, Roderich $u Institut für Chemie, Technische Universität Berlin, Straβe des 17 Juni 124/TC2, 10623, Berlin, Germany

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$a Ostash, Bohdan $u Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 4 Hrushevskoho st, Lviv, 79005, Ukraine. bohdan.ostash@lnu.edu.ua

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