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BODIPY Conjugate of Epibrassinolide as a Novel Biologically Active Probe for In Vivo Imaging

A. Starodubtseva, T. Kalachova, O. Iakovenko, V. Stoudková, V. Zhabinskii, V. Khripach, E. Ruelland, J. Martinec, L. Burketová, V. Kravets

. 2021 ; 22 (7) : . [pub] 20210330

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21018984

Grant support
N 13-03-20 National Academy of Sciences of Ukraine
BRFFR Х20УКА-023 Belarusian Republican Foundation for Fundamental Research
CZ.02.1.01/0.0/0.0/16_019/0000738 European Regional Development Fund
LM2018129 Ministerstvo Školství, Mládeže a Tělovýchovy
52010701 International Visegrad Fund

Brassinosteroids (BRs) are plant hormones of steroid nature, regulating various developmental and adaptive processes. The perception, transport, and signaling of BRs are actively studied nowadays via a wide range of biochemical and genetic tools. However, most of the knowledge about BRs intracellular localization and turnover relies on the visualization of the receptors or cellular compartments using dyes or fluorescent protein fusions. We have previously synthesized a conjugate of epibrassinolide with green fluorescent dye BODIPY (eBL-BODIPY). Here we present a detailed assessment of the compound bioactivity and its suitability as probe for in vivo visualization of BRs. We show that eBL-BODIPY rapidly penetrates epidermal cells of Arabidopsis thaliana roots and after long exposure causes physiological and transcriptomic responses similar to the natural hormone.

References provided by Crossref.org

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