• Je něco špatně v tomto záznamu ?

A PVDF electrospun antifibrotic composite for use as a glaucoma drainage implant

A. Klapstova, J. Horakova, M. Tunak, A. Shynkarenko, J. Erben, J. Hlavata, P. Bulir, J. Chvojka

. 2021 ; 119 (-) : 111637. [pub] 20201017

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21019355

Glaucoma disease therapy frequently involves the application of a glaucoma implant. This approach is effective in terms of reducing the intraocular pressure via the filtering of intraocular fluid from the anterior chamber into the drainage pathways. The basic properties of such implants comprise of long-term stability and the filtering of fluids without the occurrence of undesirable blockages. This study describes the design and production of a novel material for the treatment of glaucoma disease that is based on electrospinning technology. Non-toxic, biocompatible and non-degradable polyvinylidenefluoride (PVDF) was selected as the implant material. The research investigated the resistance of this material to the growth of a fibroblast cell line without the use of antifibrotic agents such as mitomycin C. Three different types of PVDF were electrospun separately and mixed with polyethyleneoxide (PEO), following which the degree of cell growth resistance was evaluated. It was discovered that the fiber layers that contained PVDF blended with PEO evinced a statistically significant difference in metabolic activity compared to the PURE PVDF layers. Only small cell clusters formed on the layers that were resistant to cell fibrotization. As a result of the observed clustering, a new program was developed in MATLAB software for the determination of the number of cells involved in cluster formation, which then allowed for the determination of the spatial dependence between the cells in the form of a point pattern. The study describes a simple technique for the production of composite PVDF+PEO structures suitable for use in the field of glaucoma treatment.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21019355
003      
CZ-PrNML
005      
20210830100927.0
007      
ta
008      
210728s2021 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.msec.2020.111637 $2 doi
035    __
$a (PubMed)33321675
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Klapstova, Andrea $u Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwovens and Nanofibrous Materials, Studentska 1402/2, 461 17 Liberec, Czech Republic. Electronic address: andrea.klapstova@tul.cz
245    12
$a A PVDF electrospun antifibrotic composite for use as a glaucoma drainage implant / $c A. Klapstova, J. Horakova, M. Tunak, A. Shynkarenko, J. Erben, J. Hlavata, P. Bulir, J. Chvojka
520    9_
$a Glaucoma disease therapy frequently involves the application of a glaucoma implant. This approach is effective in terms of reducing the intraocular pressure via the filtering of intraocular fluid from the anterior chamber into the drainage pathways. The basic properties of such implants comprise of long-term stability and the filtering of fluids without the occurrence of undesirable blockages. This study describes the design and production of a novel material for the treatment of glaucoma disease that is based on electrospinning technology. Non-toxic, biocompatible and non-degradable polyvinylidenefluoride (PVDF) was selected as the implant material. The research investigated the resistance of this material to the growth of a fibroblast cell line without the use of antifibrotic agents such as mitomycin C. Three different types of PVDF were electrospun separately and mixed with polyethyleneoxide (PEO), following which the degree of cell growth resistance was evaluated. It was discovered that the fiber layers that contained PVDF blended with PEO evinced a statistically significant difference in metabolic activity compared to the PURE PVDF layers. Only small cell clusters formed on the layers that were resistant to cell fibrotization. As a result of the observed clustering, a new program was developed in MATLAB software for the determination of the number of cells involved in cluster formation, which then allowed for the determination of the spatial dependence between the cells in the form of a point pattern. The study describes a simple technique for the production of composite PVDF+PEO structures suitable for use in the field of glaucoma treatment.
650    _2
$a komorová voda $7 D001082
650    12
$a glaukom $x farmakoterapie $7 D005901
650    12
$a drenážní implantáty glaukomové $7 D020327
650    _2
$a lidé $7 D006801
650    _2
$a nitrooční tlak $7 D007429
650    _2
$a mitomycin $7 D016685
655    _2
$a časopisecké články $7 D016428
700    1_
$a Horakova, Jana $u Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwovens and Nanofibrous Materials, Studentska 1402/2, 461 17 Liberec, Czech Republic
700    1_
$a Tunak, Maros $u Technical University of Liberec, Faculty of Textile Engineering, Department of Textile Evaluation, Studentska 1402/2, 461 17 Liberec, Czech Republic
700    1_
$a Shynkarenko, Andrii $u Technical University of Liberec, Faculty of Mechanical Engineering, Department of Manufacturing Systems and Automation, Studentska 1402/2, 461 17 Liberec, Czech Republic
700    1_
$a Erben, Jakub $u Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwovens and Nanofibrous Materials, Studentska 1402/2, 461 17 Liberec, Czech Republic
700    1_
$a Hlavata, Jana $u Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwovens and Nanofibrous Materials, Studentska 1402/2, 461 17 Liberec, Czech Republic
700    1_
$a Bulir, Petr $u Liberec Regional Hospital, Department of Ophthalmology, Vzdusna 1360/6, 460 01 Liberec, Czech Republic
700    1_
$a Chvojka, Jiri $u Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwovens and Nanofibrous Materials, Studentska 1402/2, 461 17 Liberec, Czech Republic
773    0_
$w MED00184559 $t Materials science & engineering. C, Materials for biological applications $x 1873-0191 $g Roč. 119, č. - (2021), s. 111637
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33321675 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210728 $b ABA008
991    __
$a 20210830100928 $b ABA008
999    __
$a ok $b bmc $g 1690230 $s 1139801
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 119 $c - $d 111637 $e 20201017 $i 1873-0191 $m Materials science & engineering. C, Materials for biological applications $n Mater Sci Eng C Mater Biol Appl $x MED00184559
LZP    __
$a Pubmed-20210728

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...