- MeSH
- Early Diagnosis MeSH
- Glaucoma * diagnosis drug therapy classification prevention & control therapy MeSH
- Aqueous Humor MeSH
- Humans MeSH
- Minimally Invasive Surgical Procedures classification methods MeSH
- Intraocular Pressure MeSH
- Eye pathology MeSH
- Visual Field Tests methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Review MeSH
Glaucoma disease therapy frequently involves the application of a glaucoma implant. This approach is effective in terms of reducing the intraocular pressure via the filtering of intraocular fluid from the anterior chamber into the drainage pathways. The basic properties of such implants comprise of long-term stability and the filtering of fluids without the occurrence of undesirable blockages. This study describes the design and production of a novel material for the treatment of glaucoma disease that is based on electrospinning technology. Non-toxic, biocompatible and non-degradable polyvinylidenefluoride (PVDF) was selected as the implant material. The research investigated the resistance of this material to the growth of a fibroblast cell line without the use of antifibrotic agents such as mitomycin C. Three different types of PVDF were electrospun separately and mixed with polyethyleneoxide (PEO), following which the degree of cell growth resistance was evaluated. It was discovered that the fiber layers that contained PVDF blended with PEO evinced a statistically significant difference in metabolic activity compared to the PURE PVDF layers. Only small cell clusters formed on the layers that were resistant to cell fibrotization. As a result of the observed clustering, a new program was developed in MATLAB software for the determination of the number of cells involved in cluster formation, which then allowed for the determination of the spatial dependence between the cells in the form of a point pattern. The study describes a simple technique for the production of composite PVDF+PEO structures suitable for use in the field of glaucoma treatment.
- MeSH
- Glaucoma Drainage Implants * MeSH
- Glaucoma * drug therapy MeSH
- Aqueous Humor MeSH
- Humans MeSH
- Mitomycin MeSH
- Intraocular Pressure MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
A practical demonstration of pH measurement in real biological samples with an in-house developed fiber-optic pH sensor system is presented. The sensor uses 8-hydroxypyrene-1,3,6-trisulfonate (HPTS) fluorescent dye as the opto-chemical transducer. The dye is immobilized in a hybrid sol-gel matrix at the tip of a tapered optical fiber. We used 405 nm and 450 nm laser diodes for the dye excitation and a photomultiplier tube as a detector. The sensor was used for the measurement of pH in human aqueous humor samples during cataract surgery. Two groups of patients were tested, one underwent conventional phacoemulsification removal of the lens while the other was subjected to femtosecond laser assisted cataract surgery (FLACS). The precision of the measurement was ±0.04 pH units. The average pH of the aqueous humor of patients subjected to FLACS and those subjected to phacoemulsification were 7.24 ± 0.17 and 7.31 ± 0.20 respectively.
- MeSH
- Phacoemulsification * MeSH
- Aqueous Humor MeSH
- Hydrogen-Ion Concentration MeSH
- Laser Therapy * MeSH
- Humans MeSH
- Prospective Studies MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
PURPOSE: Posterior polymorphous corneal dystrophy (PPCD) is characterized by abnormal proliferation of corneal endothelial cells. It was shown that TGF-β2 present in aqueous humor (AH) could help maintaining the corneal endothelium in a G1-phase-arrest state. We wanted to determine whether the levels of this protein are changed in AH of PPCD patients. METHODS: We determined the concentrations of active TGF-β2 in the AH of 29 PPCD patients (42 samples) and 40 cadaver controls (44 samples) by ELISA. For data analysis the PPCD patients were divided based on either the molecular genetic cause of their disease as PPCD1 (37 samples), PPCD3 (1 sample) and PPCDx (not linked to a known PPCD loci, 4 samples) or on the presence (17 samples) or absence (25 samples) of secondary glaucoma or on whether they had undergone penetrating keratoplasty (PK, 32 samples) or repeated PK (rePK, 7 samples). RESULTS: The level of active TGF-β2 in the AH of all PPCD patients (mean ± SD; 386.98 ± 114.88 pg/ml) in comparison to the control group (260.95 ± 112.43 pg/ml) was significantly higher (P = 0.0001). Compared to the control group, a significantly higher level of active TGF-β2 was found in the PPCD1 (P = 0.0005) and PPCDx (P = 0.0022) groups. Among patients the levels of active TGF-β2 were not significantly affected by gender, age, secondary glaucoma or by the progression of dystrophy when one or repeated PK were performed. CONCLUSION: The levels of active TGF-β2 in the AH of PPCD patients are significantly higher than control values, and thus the increased levels of TGF-β2 could be a consequence of the PPCD phenotype and can be considered as another feature characterizing this disease.
- MeSH
- Corneal Dystrophies, Hereditary metabolism MeSH
- Glaucoma metabolism MeSH
- Keratoplasty, Penetrating methods MeSH
- Aqueous Humor metabolism MeSH
- Middle Aged MeSH
- Humans MeSH
- Cornea metabolism MeSH
- Endothelium, Corneal metabolism MeSH
- Transforming Growth Factor beta2 metabolism MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
31 l. : il., tab. ; 31 cm
Patologická neovaskularizace je podkladem řady závažných očních onemocnění, zejména proliferativních retinopatií. VEGF je významný promotor angiogeneze, jehož koncentraci v tělesných tekutinách lze stanovit pomocí multiplexové analýzy ? metody, která kombinuje imunoanalýzu a průtokovou cytometrii. V současné době je zkoumána role dalších cytokinů -promotorů angiogeneze. Stanovení přítomnosti a stupně patologické oční neovaskularizace je dosud výhradně prováděno z klinického obrazu, dosud neexistuje žádná jednoduchá laboratorní metoda k posouzení závažnosti neovaskularizace. V pilotní studii jsme zjistili, že koncentrace VEGF, EGF, IL 6 a IL 10 lze měřit v nitrooční tekutině získané jednoduchou aspirací z přední komory oka. V projektu zkoumáme, zda je možné nalézt korelaci mezi koncentrací VEGF a cytokinů v nitrooční tekutině v různých klinických stadiích patologické oční neovaskularizace. Cílem práce je zavést multiplexovou analýzu jako laboratorní metodu k monitoraci patologické oční neovaskularizace; Pathological neovascularization is the base of many serious ocular diseases, particularly proliferative retinopathies. To date, the role of proangiogenic cytokines is being investigated. The multiplex analysis combining imunoanalysis and flow cytometry is used to assess its concentration in the body fluids. So far the clinical picture has been used to diagnose the occurrence and the stage of the pathological neovascularization. No simple method for measuring the severity of neovascularization is currently available. The possibility to measure the proangiogenic cytokines concentration in the ocular fluid was found out in the pilot study. The project is focused on finding the correlation between the cytokines concentration in the intraocular fluid and various clinical stages of pathological ocular neovascularization. The expected endpoint of the project is the introduction of the multiplex analysis as the laboratory method in observation and therapy of patients with proliferative diabetic retinopathy.
- MeSH
- Algorithms MeSH
- Diabetic Retinopathy complications MeSH
- Extracellular Fluid MeSH
- Immunoassay MeSH
- Aqueous Humor MeSH
- Angiogenesis Inducing Agents MeSH
- Neovascularization, Pathologic MeSH
- Promoter Regions, Genetic MeSH
- Flow Cytometry MeSH
- Vascular Endothelial Growth Factors MeSH
- Conspectus
- Ortopedie. Chirurgie. Oftalmologie
- NML Fields
- oftalmologie
- angiologie
- NML Publication type
- závěrečné zprávy o řešení grantu IGA MZ ČR
Autoři referují o výsledcích srovnávací studie mikrobiologického vyšetření komorové tekutiny po implantaci nitrooční čočky (IOČ) CORNEAL dvěma způsoby: pinzetami a zavaděčem. Metodika: Do této klinické prospektivní randomizované studie bylo zařazeno 46 očí (43 pacientů), kterým byla implantována nitrooční čočka CORNEAL ACR6D SE. U 23 očí jsme použili k implantaci zavaděč, u 23 očí pinzety. Po zavedení IOČ do kapsulárního vaku jsme standardně odstranili zbytky viskoelastického materiálu bimanuální irigací/aspirací (I/A) a odebrali vzorek komorové tekutiny na mikrobiologické vyšetření. Následovalo doplnění přední komory, intrakamerální aplikace antibiotik a hydratace ran. Výsledky: U všech odběrů byl výsledek primo kultivace negativní, v pomnožení se 3krát objevily ojedinělé kolonie Staphylococcus plazmakoaguláza negativní, které však mikrobiolog označil za velmi pravděpodobnou kontaminaci vzorku. Ve dvou z těchto tří případů byly k implantaci použity pinzety, u jednoho oka zavaděč. Vyšetření na anaeroby a mykologické vyšetření bylo ve všech případech negativní. Pooperační průběh byl u všech sledovaných očí bez komplikací, bez známek zánětlivé reakce. Závěr: Nezjistili jsme žádný rozdíl při mikrobiologickém vyšetření komorové tekutiny po implantaci IOČ CORNEAL dvěma způsoby, obě techniky jsou bezpečné.
To compare the results of the microbiological examination of aqueous humor after implantation of the intraocular lens (IOL) CORNEAL using either injector or forceps. Materials and methods: In this prospective randomized clinical study 46 eyes (43 patients) were implanted with the hydrophylic acrylic IOL CORNEAL ACR6D SE. Injector was used in 23 eyes and folding and implantation forceps in 23 eyes. After delivery of the IOL in the capsular bag, viscoelastic material was removed using bimanual I/A and the specimen for microbiological examination was obtained. The anterior chamber was filled with balanced salt solution, intracameral antibiotics were injected and the stroma alongside the primary incision and the side port incisions were lightly irrigated to form a fairly secure seal. Results: The results of primary cultivation were negative in all specimen. Only threetimes sporadic colonies of Staphylococcus plasmacoag. neg. were isolated from the liquid medium after reincubation, that were described by microbiologist as very probably contamination of the sample. At two from these three cases forceps were used for implantation, once injector. Anaerobic and mycologic examinations of all samples were negative. There was no evidence of postoperative inflammation in any case. Conclusion: Both implantation techniques proved to be safe with no significant differences comparing the microbiological examination and postoperative outcome.
- MeSH
- Diagnostic Techniques, Ophthalmological instrumentation utilization MeSH
- Glaucoma surgery therapy MeSH
- Aqueous Humor physiology MeSH
- Humans MeSH
- Sclera surgery MeSH
- Ultrasonography instrumentation utilization MeSH
- Check Tag
- Humans MeSH
- Publication type
- Book Review MeSH
- MeSH
- Antioxidants chemistry MeSH
- Research Support as Topic MeSH
- Aqueous Humor chemistry radiation effects MeSH
- Oxidoreductases chemistry MeSH
- Hydrogen Peroxide metabolism MeSH
- Cornea enzymology pathology radiation effects MeSH
- Ultraviolet Rays adverse effects MeSH
- Xanthine Oxidase chemistry MeSH
- Publication type
- Review MeSH
- MeSH
- Glaucoma MeSH
- Aqueous Humor physiology physiopathology MeSH
- Humans MeSH
- Intraocular Pressure MeSH
- Check Tag
- Humans MeSH
