-
Je něco špatně v tomto záznamu ?
Analysis of hyaluronan and its derivatives using chromatographic and mass spectrometric techniques
M. Šimek, K. Lemr, M. Hermannová, V. Havlíček
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, přehledy
- MeSH
- aminace MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- kyselina hyaluronová analýza chemie MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
The aim of this paper is to review chromatographic and mass-spectrometric methods and underline the best analytical approaches for successful analysis of various hyaluronic acid species in different types of samples. Hyaluronan-degrading enzymes and chemical depolymerization produce di- or oligosaccharides suitable for hyaluronan quantification or structural characterization of hyaluronan derivatives. Efficient purification and pre-column derivatization of hyaluronan disaccharides by reductive amination allow subnanogram quantification in biological samples. The chromatographic separation is capable to distinguish all glycosaminoglycans disaccharides and to resolve hyaluronan fragments with 2-40 monomers. Using electrospray ionization or matrix assisted laser desorption ionization, hyaluronan fragments up to 8 kDa or 41 kDa, respectively, can be observed. One- or two-dimensional chromatographic separation with higly sensitive mass-spectrometric detection is an indispensable tool for revealing substituent position, extent of modification and substitution patterns of chemically modified hyaluronan derivatives. It is essential for studying structure-biological function relationships of hyaluronan and its derivatives.
Contipro a s Dolní Dobrouč 401 56102 Dolní Dobrouč Czech Republic
Institute of Microbiology v v i The Czech Academy of Sciences Videnska 1083 Prague Czech Republic
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21019641
- 003
- CZ-PrNML
- 005
- 20210830101227.0
- 007
- ta
- 008
- 210728s2020 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.carbpol.2020.117014 $2 doi
- 035 __
- $a (PubMed)33049874
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Šimek, Matěj $u Contipro a.s., Dolní Dobrouč 401, 56102, Dolní Dobrouč, Czech Republic; Department of Analytical Chemistry, Faculty of Science, Palacký University, 17.listopadu 12, 77146, Olomouc, Czech Republic. Electronic address: matej.simek@contipro.com
- 245 10
- $a Analysis of hyaluronan and its derivatives using chromatographic and mass spectrometric techniques / $c M. Šimek, K. Lemr, M. Hermannová, V. Havlíček
- 520 9_
- $a The aim of this paper is to review chromatographic and mass-spectrometric methods and underline the best analytical approaches for successful analysis of various hyaluronic acid species in different types of samples. Hyaluronan-degrading enzymes and chemical depolymerization produce di- or oligosaccharides suitable for hyaluronan quantification or structural characterization of hyaluronan derivatives. Efficient purification and pre-column derivatization of hyaluronan disaccharides by reductive amination allow subnanogram quantification in biological samples. The chromatographic separation is capable to distinguish all glycosaminoglycans disaccharides and to resolve hyaluronan fragments with 2-40 monomers. Using electrospray ionization or matrix assisted laser desorption ionization, hyaluronan fragments up to 8 kDa or 41 kDa, respectively, can be observed. One- or two-dimensional chromatographic separation with higly sensitive mass-spectrometric detection is an indispensable tool for revealing substituent position, extent of modification and substitution patterns of chemically modified hyaluronan derivatives. It is essential for studying structure-biological function relationships of hyaluronan and its derivatives.
- 650 _2
- $a aminace $7 D000586
- 650 _2
- $a kyselina hyaluronová $x analýza $x chemie $7 D006820
- 650 _2
- $a hmotnostní spektrometrie s elektrosprejovou ionizací $x metody $7 D021241
- 650 _2
- $a spektrometrie hmotnostní - ionizace laserem za účasti matrice $x metody $7 D019032
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a přehledy $7 D016454
- 700 1_
- $a Lemr, Karel $u Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, 17.listopadu 12, Olomouc, 77147, Czech Republic; Institute of Microbiology, v.v.i., The Czech Academy of Sciences, Videnska, 1083, Prague, Czech Republic
- 700 1_
- $a Hermannová, Martina $u Contipro a.s., Dolní Dobrouč 401, 56102, Dolní Dobrouč, Czech Republic
- 700 1_
- $a Havlíček, Vladimír $u Department of Analytical Chemistry, Faculty of Science, Palacký University, 17.listopadu 12, 77146, Olomouc, Czech Republic; Institute of Microbiology, v.v.i., The Czech Academy of Sciences, Videnska, 1083, Prague, Czech Republic
- 773 0_
- $w MED00006149 $t Carbohydrate polymers $x 1879-1344 $g Roč. 250, č. - (2020), s. 117014
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33049874 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20210728 $b ABA008
- 991 __
- $a 20210830101227 $b ABA008
- 999 __
- $a ok $b bmc $g 1690455 $s 1140087
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 250 $c - $d 117014 $e 20200901 $i 1879-1344 $m Carbohydrate polymers $n Carbohydr Polym $x MED00006149
- LZP __
- $a Pubmed-20210728
