Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Single-Cell RNA Profiling Reveals Adipocyte to Macrophage Signaling Sufficient to Enhance Thermogenesis

F. Henriques, AH. Bedard, A. Guilherme, M. Kelly, J. Chi, P. Zhang, LM. Lifshitz, K. Bellvé, LA. Rowland, B. Yenilmez, S. Kumar, Y. Wang, J. Luban, LS. Weinstein, JD. Lin, P. Cohen, MP. Czech

. 2020 ; 32 (5) : 107998. [pub] 20200804

Language English Country United States

Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc21020269

Grant support
R01 DK116056 NIDDK NIH HHS - United States
R01 DK120649 NIDDK NIH HHS - United States
R37 AI147868 NIAID NIH HHS - United States
R01 DK102456 NIDDK NIH HHS - United States
P30 DK020541 NIDDK NIH HHS - United States
R37 DK030898 NIDDK NIH HHS - United States
R01 DK030898 NIDDK NIH HHS - United States
R01 DK103047 NIDDK NIH HHS - United States

Adipocytes deficient in fatty acid synthase (iAdFASNKO) emit signals that mimic cold exposure to enhance the appearance of thermogenic beige adipocytes in mouse inguinal white adipose tissues (iWATs). Both cold exposure and iAdFASNKO upregulate the sympathetic nerve fiber (SNF) modulator Neuregulin 4 (Nrg4), activate SNFs, and require adipocyte cyclic AMP/protein kinase A (cAMP/PKA) signaling for beige adipocyte appearance, as it is blocked by adipocyte Gsα deficiency. Surprisingly, however, in contrast to cold-exposed mice, neither iWAT denervation nor Nrg4 loss attenuated adipocyte browning in iAdFASNKO mice. Single-cell transcriptomic analysis of iWAT stromal cells revealed increased macrophages displaying gene expression signatures of the alternately activated type in iAdFASNKO mice, and their depletion abrogated iWAT beiging. Altogether, these findings reveal that divergent cellular pathways are sufficient to cause adipocyte browning. Importantly, adipocyte signaling to enhance alternatively activated macrophages in iAdFASNKO mice is associated with enhanced adipose thermogenesis independent of the sympathetic neuron involvement this process requires in the cold.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21020269
003      
CZ-PrNML
005      
20210830101904.0
007      
ta
008      
210728s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.celrep.2020.107998 $2 doi
035    __
$a (PubMed)32755590
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Henriques, Felipe $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
245    10
$a Single-Cell RNA Profiling Reveals Adipocyte to Macrophage Signaling Sufficient to Enhance Thermogenesis / $c F. Henriques, AH. Bedard, A. Guilherme, M. Kelly, J. Chi, P. Zhang, LM. Lifshitz, K. Bellvé, LA. Rowland, B. Yenilmez, S. Kumar, Y. Wang, J. Luban, LS. Weinstein, JD. Lin, P. Cohen, MP. Czech
520    9_
$a Adipocytes deficient in fatty acid synthase (iAdFASNKO) emit signals that mimic cold exposure to enhance the appearance of thermogenic beige adipocytes in mouse inguinal white adipose tissues (iWATs). Both cold exposure and iAdFASNKO upregulate the sympathetic nerve fiber (SNF) modulator Neuregulin 4 (Nrg4), activate SNFs, and require adipocyte cyclic AMP/protein kinase A (cAMP/PKA) signaling for beige adipocyte appearance, as it is blocked by adipocyte Gsα deficiency. Surprisingly, however, in contrast to cold-exposed mice, neither iWAT denervation nor Nrg4 loss attenuated adipocyte browning in iAdFASNKO mice. Single-cell transcriptomic analysis of iWAT stromal cells revealed increased macrophages displaying gene expression signatures of the alternately activated type in iAdFASNKO mice, and their depletion abrogated iWAT beiging. Altogether, these findings reveal that divergent cellular pathways are sufficient to cause adipocyte browning. Importantly, adipocyte signaling to enhance alternatively activated macrophages in iAdFASNKO mice is associated with enhanced adipose thermogenesis independent of the sympathetic neuron involvement this process requires in the cold.
650    _2
$a béžové tukové buňky $x metabolismus $7 D000069797
650    _2
$a hnědá tuková tkáň $x metabolismus $7 D002001
650    _2
$a bílá tuková tkáň $x metabolismus $7 D052436
650    _2
$a zvířata $7 D000818
650    _2
$a polarita buněk $7 D016764
650    _2
$a nízká teplota $7 D003080
650    _2
$a AMP cyklický $x metabolismus $7 D000242
650    _2
$a denervace $7 D003714
650    _2
$a syntázy mastných kyselin $x metabolismus $7 D064429
650    _2
$a aktivace makrofágů $7 D008262
650    _2
$a makrofágy $x metabolismus $7 D008264
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a myši inbrední C57BL $7 D008810
650    _2
$a myši knockoutované $7 D018345
650    _2
$a neureguliny $x nedostatek $x metabolismus $7 D020880
650    _2
$a fenotyp $7 D010641
650    _2
$a RNA $x metabolismus $7 D012313
650    12
$a signální transdukce $7 D015398
650    12
$a analýza jednotlivých buněk $7 D059010
650    12
$a termogeneze $7 D022722
650    _2
$a uncoupling protein 1 $x genetika $x metabolismus $7 D000071256
650    _2
$a upregulace $x genetika $7 D015854
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a Research Support, N.I.H., Intramural $7 D052060
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Bedard, Alexander H $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Guilherme, Adilson $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Kelly, Mark $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Chi, Jingyi $u Laboratory of Molecular Metabolism, The Rockefeller University, New York, NY, USA
700    1_
$a Zhang, Peng $u Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, MI, USA; Department of Cell and Developmental Biology, University of Michigan Medical Center, Ann Arbor, MI, USA
700    1_
$a Lifshitz, Lawrence M $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Bellvé, Karl $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Rowland, Leslie A $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Yenilmez, Batuhan $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Kumar, Shreya $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Wang, Yetao $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Luban, Jeremy $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA
700    1_
$a Weinstein, Lee S $u Metabolic Diseases Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
700    1_
$a Lin, Jiandie D $u Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, MI, USA; Department of Cell and Developmental Biology, University of Michigan Medical Center, Ann Arbor, MI, USA
700    1_
$a Cohen, Paul $u Laboratory of Molecular Metabolism, The Rockefeller University, New York, NY, USA
700    1_
$a Czech, Michael P $u Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA. Electronic address: michael.czech@umassmed.edu
773    0_
$w MED00188029 $t Cell reports $x 2211-1247 $g Roč. 32, č. 5 (2020), s. 107998
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32755590 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210728 $b ABA008
991    __
$a 20210830101904 $b ABA008
999    __
$a ok $b bmc $g 1690949 $s 1140715
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 32 $c 5 $d 107998 $e 20200804 $i 2211-1247 $m Cell reports $n Cell Rep $x MED00188029
GRA    __
$a R01 DK116056 $p NIDDK NIH HHS $2 United States
GRA    __
$a R01 DK120649 $p NIDDK NIH HHS $2 United States
GRA    __
$a R37 AI147868 $p NIAID NIH HHS $2 United States
GRA    __
$a R01 DK102456 $p NIDDK NIH HHS $2 United States
GRA    __
$a P30 DK020541 $p NIDDK NIH HHS $2 United States
GRA    __
$a R37 DK030898 $p NIDDK NIH HHS $2 United States
GRA    __
$a R01 DK030898 $p NIDDK NIH HHS $2 United States
GRA    __
$a R01 DK103047 $p NIDDK NIH HHS $2 United States
LZP    __
$a Pubmed-20210728
Back

Find record

Citation metrics

Archiving options