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Does the renal expression of Toll-like receptors play a role in patients with IgA nephropathy

H. Ciferska, E. Honsova, A. Lodererova, Z. Hruskova, M. Neprasova, J. Vachek, M. Suchanek, T. Zima, R. Coppo, V. Tesar, J. Novak, D. Maixnerova

. 2020 ; 33 (2) : 307-316. [pub] 20190905

Jazyk angličtina Země Itálie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21020660

Grantová podpora
023728 Ministry of Health of the Czech Republic
LH15168 Ministry of Health of the Czech Republic
LM2015073 Research Infrastructure NanoEnviCz supported by the Ministry of Education, Youth and Sports of the Czech Republic
R01 DK082753 NIDDK NIH HHS - United States
R56 DK078244 NIDDK NIH HHS - United States
DK078244 National Institutes of Health, Czech Republic
DRO VFN 64165 Ministry of Health of the Czech Republic
PROGRES Q25/LF1 Ministry of Health of the Czech Republic
R01 DK078244 NIDDK NIH HHS - United States
DK082753 National Institutes of Health, Czech Republic

The onset of IgA nephropathy (IgAN), characterized by glomerular deposition of IgA-containing immune complexes, is often associated with synpharyngitic hematuria. Innate immune responses mediated by Toll-like receptors (TLR) may play a role in IgAN onset and/or progression. Here, we assessed the expression of TLR 4, 7, 8, and 9 in renal-biopsy specimens from patients with IgAN, with different degree of proteinuria and eGFR, compared with normal-kidney and disease-control tissues (ANCA-associated vasculitis). Renal-biopsy specimens from 34 patients with IgAN and 7 patients with ANCA-associated vasculitis were used. In addition, we used 15 healthy portions of renal-tissue specimens from kidneys after nephrectomy for cancer as control specimens. Expression of TLR 4, 7, 8, and 9 was assessed using immunohistochemical staining of paraffin-embedded renal-biopsy tissue specimens with specific antibodies and evaluated semiquantitatively by light microscopy. Linear discriminant analysis (LDA) was used to test whether intrarenal staining of TLR 4, 7, 8, and 9 distinguished patients with IgAN from controls or correlated with eGFR and/or proteinuria. eGFR was calculated using the creatinine-based formula. Moreover, the biopsies from patients with IgAN were scored according to the Oxford Classification. LDA showed that staining for TLR 4, 7, 8, and 9 was more intense in specimens from IgAN patients compared to normal kidney tissues. The intensity of intrarenal staining of TLRs discriminated four groups of IgAN patients with different eGFR and proteinuria and MEST scoring.

Citace poskytuje Crossref.org

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