• Je něco špatně v tomto záznamu ?

Identification of Immune Cell Infiltration in Murine Pheochromocytoma during Combined Mannan-BAM, TLR Ligand, and Anti-CD40 Antibody-Based Immunotherapy

O. Uher, TT. Huynh, B. Zhu, LA. Horn, V. Caisova, K. Hadrava Vanova, R. Medina, H. Wang, C. Palena, J. Chmelar, Z. Zhuang, J. Zenka, K. Pacak

. 2021 ; 13 (16) : . [pub] 20210805

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21024203

Immunotherapy has become an essential component in cancer treatment. However, the majority of solid metastatic cancers, such as pheochromocytoma, are resistant to this approach. Therefore, understanding immune cell composition in primary and distant metastatic tumors is important for therapeutic intervention and diagnostics. Combined mannan-BAM, TLR ligand, and anti-CD40 antibody-based intratumoral immunotherapy (MBTA therapy) previously resulted in the complete eradication of murine subcutaneous pheochromocytoma and demonstrated a systemic antitumor immune response in a metastatic model. Here, we further evaluated this systemic effect using a bilateral pheochromocytoma model, performing MBTA therapy through injection into the primary tumor and using distant (non-injected) tumors to monitor size changes and detailed immune cell infiltration. MBTA therapy suppressed the growth of not only injected but also distal tumors and prolonged MBTA-treated mice survival. Our flow cytometry analysis showed that MBTA therapy led to increased recruitment of innate and adaptive immune cells in both tumors and the spleen. Moreover, adoptive CD4+ T cell transfer from successfully MBTA-treated mice (i.e., subcutaneous pheochromocytoma) demonstrates the importance of these cells in long-term immunological memory. In summary, this study unravels further details on the systemic effect of MBTA therapy and its use for tumor and metastasis reduction or even elimination.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21024203
003      
CZ-PrNML
005      
20211013134024.0
007      
ta
008      
211006s2021 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/cancers13163942 $2 doi
035    __
$a (PubMed)34439097
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Uher, Ondrej $u Section on Medical Neuroendocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA $u Department of Medical Biology, Faculty of Science, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic
245    10
$a Identification of Immune Cell Infiltration in Murine Pheochromocytoma during Combined Mannan-BAM, TLR Ligand, and Anti-CD40 Antibody-Based Immunotherapy / $c O. Uher, TT. Huynh, B. Zhu, LA. Horn, V. Caisova, K. Hadrava Vanova, R. Medina, H. Wang, C. Palena, J. Chmelar, Z. Zhuang, J. Zenka, K. Pacak
520    9_
$a Immunotherapy has become an essential component in cancer treatment. However, the majority of solid metastatic cancers, such as pheochromocytoma, are resistant to this approach. Therefore, understanding immune cell composition in primary and distant metastatic tumors is important for therapeutic intervention and diagnostics. Combined mannan-BAM, TLR ligand, and anti-CD40 antibody-based intratumoral immunotherapy (MBTA therapy) previously resulted in the complete eradication of murine subcutaneous pheochromocytoma and demonstrated a systemic antitumor immune response in a metastatic model. Here, we further evaluated this systemic effect using a bilateral pheochromocytoma model, performing MBTA therapy through injection into the primary tumor and using distant (non-injected) tumors to monitor size changes and detailed immune cell infiltration. MBTA therapy suppressed the growth of not only injected but also distal tumors and prolonged MBTA-treated mice survival. Our flow cytometry analysis showed that MBTA therapy led to increased recruitment of innate and adaptive immune cells in both tumors and the spleen. Moreover, adoptive CD4+ T cell transfer from successfully MBTA-treated mice (i.e., subcutaneous pheochromocytoma) demonstrates the importance of these cells in long-term immunological memory. In summary, this study unravels further details on the systemic effect of MBTA therapy and its use for tumor and metastasis reduction or even elimination.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Huynh, Thanh-Truc $u Section on Medical Neuroendocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
700    1_
$a Zhu, Boqun $u Section on Medical Neuroendocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA $u Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China
700    1_
$a Horn, Lucas A $u Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
700    1_
$a Caisova, Veronika $u Section on Medical Neuroendocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
700    1_
$a Hadrava Vanova, Katerina $u Section on Medical Neuroendocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
700    1_
$a Medina, Rogelio $u Neuro-Oncology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
700    1_
$a Wang, Herui $u Neuro-Oncology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
700    1_
$a Palena, Claudia $u Laboratory of Tumor Immunology and Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD 20892, USA
700    1_
$a Chmelar, Jindrich $u Department of Medical Biology, Faculty of Science, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic
700    1_
$a Zhuang, Zhengping $u Neuro-Oncology Branch, National Cancer Institute, NIH, Bethesda, MD 20892, USA
700    1_
$a Zenka, Jan $u Department of Medical Biology, Faculty of Science, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic
700    1_
$a Pacak, Karel $u Section on Medical Neuroendocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH, Bethesda, MD 20892, USA
773    0_
$w MED00173178 $t Cancers $x 2072-6694 $g Roč. 13, č. 16 (2021)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34439097 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20211006 $b ABA008
991    __
$a 20211013134021 $b ABA008
999    __
$a ind $b bmc $g 1708231 $s 1144700
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 13 $c 16 $e 20210805 $i 2072-6694 $m Cancers $n Cancers $x MED00173178
LZP    __
$a Pubmed-20211006

Najít záznam

Citační ukazatele

Možnosti archivace