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Localization of SARS-CoV-2 Capping Enzymes Revealed by an Antibody against the nsp10 Subunit

V. Horova, B. Landova, J. Hodek, K. Chalupsky, P. Krafcikova, D. Chalupska, V. Duchoslav, J. Weber, E. Boura, M. Klima

. 2021 ; 13 (8) : . [pub] 20210729

Language English Country Switzerland

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc21025293

Grant support
21-25280S Grantová Agentura České Republiky

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2'-O MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2'-O RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.

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