Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Anillin propels myosin-independent constriction of actin rings

O. Kučera, V. Siahaan, D. Janda, SH. Dijkstra, E. Pilátová, E. Zatecka, S. Diez, M. Braun, Z. Lansky

. 2021 ; 12 (1) : 4595. [pub] 20210728

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21025296

Constriction of the cytokinetic ring, a circular structure of actin filaments, is an essential step during cell division. Mechanical forces driving the constriction are attributed to myosin motor proteins, which slide actin filaments along each other. However, in multiple organisms, ring constriction has been reported to be myosin independent. How actin rings constrict in the absence of motor activity remains unclear. Here, we demonstrate that anillin, a non-motor actin crosslinker, indispensable during cytokinesis, autonomously propels the contractility of actin bundles. Anillin generates contractile forces of tens of pico-Newtons to maximise the lengths of overlaps between bundled actin filaments. The contractility is enhanced by actin disassembly. When multiple actin filaments are arranged into a ring, this contractility leads to ring constriction. Our results indicate that passive actin crosslinkers can substitute for the activity of molecular motors to generate contractile forces in a variety of actin networks, including the cytokinetic ring.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21025296
003      
CZ-PrNML
005      
20240624150911.0
007      
ta
008      
211013s2021 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41467-021-24474-1 $2 doi
035    __
$a (PubMed)34321459
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Kučera, Ondřej $u Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Vestec, Prague West, Czechia $u CytoMorpho Lab, Laboratoire Physiologie Cellulaire & Végétale, Institut de recherche interdisciplinaire de Grenoble, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Grenoble, France
245    10
$a Anillin propels myosin-independent constriction of actin rings / $c O. Kučera, V. Siahaan, D. Janda, SH. Dijkstra, E. Pilátová, E. Zatecka, S. Diez, M. Braun, Z. Lansky
520    9_
$a Constriction of the cytokinetic ring, a circular structure of actin filaments, is an essential step during cell division. Mechanical forces driving the constriction are attributed to myosin motor proteins, which slide actin filaments along each other. However, in multiple organisms, ring constriction has been reported to be myosin independent. How actin rings constrict in the absence of motor activity remains unclear. Here, we demonstrate that anillin, a non-motor actin crosslinker, indispensable during cytokinesis, autonomously propels the contractility of actin bundles. Anillin generates contractile forces of tens of pico-Newtons to maximise the lengths of overlaps between bundled actin filaments. The contractility is enhanced by actin disassembly. When multiple actin filaments are arranged into a ring, this contractility leads to ring constriction. Our results indicate that passive actin crosslinkers can substitute for the activity of molecular motors to generate contractile forces in a variety of actin networks, including the cytokinetic ring.
650    _2
$a mikrofilamenta $x metabolismus $7 D008841
650    _2
$a aktiny $x metabolismus $7 D000199
650    _2
$a aktomyosin $x metabolismus $7 D000205
650    _2
$a zvířata $7 D000818
650    _2
$a buněčné dělení $7 D002455
650    _2
$a kontraktilní proteiny $x genetika $x metabolismus $7 D003285
650    _2
$a cytokineze $7 D048749
650    _2
$a Drosophila melanogaster $x metabolismus $7 D004331
650    _2
$a lidé $7 D006801
650    _2
$a mikrofilamentové proteiny $7 D008840
650    _2
$a myosiny $x metabolismus $7 D009218
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Siahaan, Valerie $u Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Vestec, Prague West, Czechia
700    1_
$a Janda, Daniel $u Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Vestec, Prague West, Czechia
700    1_
$a Dijkstra, Sietske H $u Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Vestec, Prague West, Czechia
700    1_
$a Pilátová, Eliška $u Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Vestec, Prague West, Czechia
700    1_
$a Žatecká, Eva $u Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Vestec, Prague West, Czechia $7 xx0319144
700    1_
$a Diez, Stefan $u B CUBE - Center for Molecular Bioengineering, TU Dresden, Dresden, Germany $u Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany $u Cluster of Excellence Physics of Life, Technische Universität Dresden, Dresden, Germany
700    1_
$a Braun, Marcus $u Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Vestec, Prague West, Czechia. marcus.braun@ibt.cas.cz
700    1_
$a Lansky, Zdenek $u Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Vestec, Prague West, Czechia. zdenek.lansky@ibt.cas.cz
773    0_
$w MED00184850 $t Nature communications $x 2041-1723 $g Roč. 12, č. 1 (2021), s. 4595
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34321459 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211013 $b ABA008
991    __
$a 20240624150909 $b ABA008
999    __
$a ok $b bmc $g 1714377 $s 1145803
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 12 $c 1 $d 4595 $e 20210728 $i 2041-1723 $m Nature communications $n Nat Commun $x MED00184850
LZP    __
$a Pubmed-20211013

Najít záznam

Citační ukazatele

Možnosti archivace