-
Je něco špatně v tomto záznamu ?
Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions
M. Hrala, J. Bosák, L. Micenková, J. Křenová, M. Lexa, V. Pirková, Z. Tomáštíková, I. Koláčková, D. Šmajs
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, randomizované kontrolované studie veterinární, práce podpořená grantem
PubMed Central od 1976 do Před 6 měsíci
Europe PubMed Central od 1976 do Před 6 měsíci
Open Access Digital Library od 1953-01-01
Odkazy
PubMed
33962981
DOI
10.1128/aem.03121-20
Knihovny.cz E-zdroje
- MeSH
- bakteriální toxiny * metabolismus MeSH
- bakteriociny metabolismus terapeutické užití MeSH
- Escherichia coli * účinky léků genetika metabolismus MeSH
- faktory virulence genetika MeSH
- feces mikrobiologie MeSH
- infekce vyvolané Escherichia coli mikrobiologie prevence a kontrola veterinární MeSH
- nemoci prasat mikrobiologie prevence a kontrola MeSH
- prasata MeSH
- probiotika terapeutické užití MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- randomizované kontrolované studie veterinární MeSH
Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) strains are the causative agents of severe foodborne diseases in both humans and animals. In this study, porcine pathogenic E. coli strains (n = 277) as well as porcine commensal strains (n = 188) were tested for their susceptibilities to 34 bacteriocin monoproducers to identify the most suitable bacteriocin types inhibiting porcine pathogens. Under in vitro conditions, the set of pathogenic E. coli strains was found to be significantly more susceptible to the majority of tested bacteriocins than commensal E. coli. Based on the production of bacteriocins with specific activity against pathogens, three potentially probiotic commensal E. coli strains of human origin were selected. These strains were found to be able to outcompete ETEC strains expressing F4 or F18 fimbriae in liquid culture and also decreased the severity and duration of diarrhea in piglets during experimental ETEC infection as well as pathogen numbers on the last day of in vivo experimentation. While the extents of the probiotic effect were different for each strain, the cocktail of all three strains showed the most pronounced beneficial effects, suggesting synergy between the tested E. coli strains. IMPORTANCE Increasing levels of antibiotic resistance among bacteria also increase the need for alternatives to conventional antibiotic treatment. Pathogenic Escherichia coli represents a major diarrheic infectious agent of piglets in their postweaning period; however, available measures to control these infections are limited. This study describes three novel E. coli strains producing antimicrobial compounds (bacteriocins) that actively inhibit a majority of toxigenic E. coli strains. The beneficial effect of three potentially probiotic E. coli strains was demonstrated under both in vitro and in vivo conditions. The novel probiotic candidates may be used as prophylaxis during piglets' postweaning period to overcome common infections caused by E. coli.
Department of Biology Faculty of Medicine Masaryk University Brno Czech Republic
Faculty of Informatics Masaryk University Brno Czech Republic
RECETOX Center Faculty of Science Masaryk University Brno Czech Republic
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21025526
- 003
- CZ-PrNML
- 005
- 20211026133731.0
- 007
- ta
- 008
- 211013s2021 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1128/AEM.03121-20 $2 doi
- 035 __
- $a (PubMed)33962981
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Hrala, Matěj $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
- 245 10
- $a Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions / $c M. Hrala, J. Bosák, L. Micenková, J. Křenová, M. Lexa, V. Pirková, Z. Tomáštíková, I. Koláčková, D. Šmajs
- 520 9_
- $a Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) strains are the causative agents of severe foodborne diseases in both humans and animals. In this study, porcine pathogenic E. coli strains (n = 277) as well as porcine commensal strains (n = 188) were tested for their susceptibilities to 34 bacteriocin monoproducers to identify the most suitable bacteriocin types inhibiting porcine pathogens. Under in vitro conditions, the set of pathogenic E. coli strains was found to be significantly more susceptible to the majority of tested bacteriocins than commensal E. coli. Based on the production of bacteriocins with specific activity against pathogens, three potentially probiotic commensal E. coli strains of human origin were selected. These strains were found to be able to outcompete ETEC strains expressing F4 or F18 fimbriae in liquid culture and also decreased the severity and duration of diarrhea in piglets during experimental ETEC infection as well as pathogen numbers on the last day of in vivo experimentation. While the extents of the probiotic effect were different for each strain, the cocktail of all three strains showed the most pronounced beneficial effects, suggesting synergy between the tested E. coli strains. IMPORTANCE Increasing levels of antibiotic resistance among bacteria also increase the need for alternatives to conventional antibiotic treatment. Pathogenic Escherichia coli represents a major diarrheic infectious agent of piglets in their postweaning period; however, available measures to control these infections are limited. This study describes three novel E. coli strains producing antimicrobial compounds (bacteriocins) that actively inhibit a majority of toxigenic E. coli strains. The beneficial effect of three potentially probiotic E. coli strains was demonstrated under both in vitro and in vivo conditions. The novel probiotic candidates may be used as prophylaxis during piglets' postweaning period to overcome common infections caused by E. coli.
- 650 _2
- $a zvířata $7 D000818
- 650 12
- $a bakteriální toxiny $x metabolismus $7 D001427
- 650 _2
- $a bakteriociny $x metabolismus $x terapeutické užití $7 D001430
- 650 12
- $a Escherichia coli $x účinky léků $x genetika $x metabolismus $7 D004926
- 650 _2
- $a infekce vyvolané Escherichia coli $x mikrobiologie $x prevence a kontrola $x veterinární $7 D004927
- 650 _2
- $a feces $x mikrobiologie $7 D005243
- 650 _2
- $a probiotika $x terapeutické užití $7 D019936
- 650 _2
- $a prasata $7 D013552
- 650 _2
- $a nemoci prasat $x mikrobiologie $x prevence a kontrola $7 D013553
- 650 _2
- $a faktory virulence $x genetika $7 D037521
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a randomizované kontrolované studie veterinární $7 D000081262
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Bosák, Juraj $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
- 700 1_
- $a Micenková, Lenka $u RECETOX Center, Faculty of Science, Masaryk University, Brno, Czech Republic
- 700 1_
- $a Křenová, Jitka $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
- 700 1_
- $a Lexa, Matej $u Faculty of Informatics, Masaryk University, Brno, Czech Republic
- 700 1_
- $a Pirková, Viktória $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
- 700 1_
- $a Tomáštíková, Zuzana $u Veterinary Research Institute, Brno, Czech Republic
- 700 1_
- $a Koláčková, Ivana $u Veterinary Research Institute, Brno, Czech Republic
- 700 1_
- $a Šmajs, David $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
- 773 0_
- $w MED00000487 $t Applied and environmental microbiology $x 1098-5336 $g Roč. 87, č. 14 (2021), s. e0312120
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33962981 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20211013 $b ABA008
- 991 __
- $a 20211026133737 $b ABA008
- 999 __
- $a ok $b bmc $g 1714540 $s 1146033
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 87 $c 14 $d e0312120 $e 20210625 $i 1098-5336 $m Applied and environmental microbiology $n Appl Environ Microbiol $x MED00000487
- LZP __
- $a Pubmed-20211013