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High-Throughput Fluorescent Assay for Inhibitor Screening of Proteases from RNA Viruses

B. Cihlova, A. Huskova, J. Böserle, R. Nencka, E. Boura, J. Silhan

. 2021 ; 26 (13) : . [pub] 20210622

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21025549

Grantová podpora
CZ.02.1.01/0.0/0.0/16_019/0000729 European Regional Development Fund
RVO: 61388963 Akademie Věd České Republiky

Spanish flu, polio epidemics, and the ongoing COVID-19 pandemic are the most profound examples of severe widespread diseases caused by RNA viruses. The coronavirus pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) demands affordable and reliable assays for testing antivirals. To test inhibitors of viral proteases, we have developed an inexpensive high-throughput assay based on fluorescent energy transfer (FRET). We assayed an array of inhibitors for papain-like protease from SARS-CoV-2 and validated it on protease from the tick-borne encephalitis virus to emphasize its versatility. The reaction progress is monitored as loss of FRET signal of the substrate. This robust and reproducible assay can be used for testing the inhibitors in 96- or 384-well plates.

Citace poskytuje Crossref.org

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