• Je něco špatně v tomto záznamu ?

Monitoring the Kinase Activity of Heme-Based Oxygen Sensors and Its Dependence on O2 and Other Ligands Using Phos-Tag Electrophoresis

J. Vávra, A. Sergunin, T. Shimizu, M. Martínková

. 2023 ; 2648 (-) : 63-73. [pub] -

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc23010721

The nonradioactive method, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in the presence of Phos-tag (Phos-tag electrophoresis), is used to evaluate a kinase autophosphorylation and/or phosphotransfer reaction from a kinase/ATP to its protein substrate. This method outperforms radioisotope methods using [32P]ATP for detecting trace amounts of phosphorylated protein in fresh protein preparations. Phos-tag electrophoresis has been used to perform detailed analyses of the kinase activity of a heme-based oxygen sensor-specifically, a globin-coupled histidine kinase from the soil bacterium Anaeromyxobacter sp. Fw109-5 (AfGcHK).

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc23010721
003      
CZ-PrNML
005      
20230801132611.0
007      
ta
008      
230718s2023 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1007/978-1-0716-3080-8_5 $2 doi
035    __
$a (PubMed)37039985
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Vávra, Jakub $u Department of Biochemistry, Faculty of Science, Charles University, Prague 2, Czech Republic
245    10
$a Monitoring the Kinase Activity of Heme-Based Oxygen Sensors and Its Dependence on O2 and Other Ligands Using Phos-Tag Electrophoresis / $c J. Vávra, A. Sergunin, T. Shimizu, M. Martínková
520    9_
$a The nonradioactive method, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in the presence of Phos-tag (Phos-tag electrophoresis), is used to evaluate a kinase autophosphorylation and/or phosphotransfer reaction from a kinase/ATP to its protein substrate. This method outperforms radioisotope methods using [32P]ATP for detecting trace amounts of phosphorylated protein in fresh protein preparations. Phos-tag electrophoresis has been used to perform detailed analyses of the kinase activity of a heme-based oxygen sensor-specifically, a globin-coupled histidine kinase from the soil bacterium Anaeromyxobacter sp. Fw109-5 (AfGcHK).
650    12
$a hem $x metabolismus $7 D006418
650    _2
$a ligandy $7 D008024
650    12
$a proteiny $7 D011506
650    _2
$a Bacteria $x metabolismus $7 D001419
650    _2
$a elektroforéza v polyakrylamidovém gelu $7 D004591
650    _2
$a kyslík $x metabolismus $7 D010100
650    _2
$a adenosintrifosfát $x metabolismus $7 D000255
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Sergunin, Artur $u Department of Biochemistry, Faculty of Science, Charles University, Prague 2, Czech Republic
700    1_
$a Shimizu, Toru $u Department of Biochemistry, Faculty of Science, Charles University, Prague 2, Czech Republic
700    1_
$a Martínková, Markéta $u Department of Biochemistry, Faculty of Science, Charles University, Prague 2, Czech Republic. marketa.martinkova@natur.cuni.cz
773    0_
$w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 2648, č. - (2023), s. 63-73
856    41
$u https://pubmed.ncbi.nlm.nih.gov/37039985 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230718 $b ABA008
991    __
$a 20230801132608 $b ABA008
999    __
$a ok $b bmc $g 1963269 $s 1196986
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2023 $b 2648 $c - $d 63-73 $e - $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
LZP    __
$a Pubmed-20230718

Najít záznam

Citační ukazatele

    Možnosti archivace