Binding of p53 and its core domain to supercoiled DNA
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11168396
DOI
10.1046/j.1432-1327.2001.01898.x
PII: ejb1898
Knihovny.cz E-zdroje
- MeSH
- Baculoviridae metabolismus MeSH
- DNA chemie metabolismus MeSH
- elektroforéza v agarovém gelu MeSH
- kompetitivní vazba MeSH
- konformace nukleové kyseliny MeSH
- nádorový supresorový protein p53 chemie metabolismus MeSH
- plazmidy metabolismus MeSH
- superhelikální DNA metabolismus MeSH
- terciární struktura proteinů MeSH
- vazba proteinů MeSH
- vazebná místa MeSH
- vysoká teplota MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA MeSH
- nádorový supresorový protein p53 MeSH
- superhelikální DNA MeSH
We have compared the binding of human full-length p53 protein (p53; expressed in bacteria and insects) and its isolated core domain (p53CD, amino acids 94-312; expressed in bacteria) to negatively supercoiled (sc) DNA using gel electrophoresis and immunoblotting. Significant differences were observed; p53CD produced a relatively small and continuous retardation of scDNA, in contrast to the ladder of distinct bands formed by p53 in agarose gels. The ladder produced by full-length protein expressed in bacteria (p53b) was similar to that observed earlier with protein expressed in insect cells (p53i). Competition between scDNAs and their linearized (lin) forms showed a preference for scDNAs by both p53 and p53CD, but the ratios characterizing the distribution of the protein between sc and lin pBluescript DNAs were substantially higher for p53 (sc/lin > 60 in p53b) than for p53CD (sc/lin approximately 4). Strong binding of p53 to scDNA lacking the p53 consensus sequence may represent a new p53-binding mode, which we tentatively denote supercoil-selective (SCS) binding. This binding requires both the C-terminal domain and the core domain. Targets of this binding may include: (a) DNA segments defined both by the nucleotide sequence and local topology, and/or (b) strand crossings and/or bending. The binding preference of p53CD for scDNA may be due to the known nonspecific binding to internal single-stranded regions in scDNA (absent in relaxed DNA molecules) and/or to SCS binding albeit with reduced affinity due to the absence of contributions from other p53 domains.
Citace poskytuje Crossref.org
The Rich World of p53 DNA Binding Targets: The Role of DNA Structure
Recognition of Local DNA Structures by p53 Protein
Preferential binding of hot spot mutant p53 proteins to supercoiled DNA in vitro and in cells
Role of tumor suppressor p53 domains in selective binding to supercoiled DNA