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Heptabladed β-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system
E. Fujdiarová, J. Houser, P. Dobeš, G. Paulíková, N. Kondakov, L. Kononov, P. Hyršl, M. Wimmerová
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 2005 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2005-01-01 do Před 1 rokem
Wiley Free Content
od 2005 do Před 1 rokem
PubMed
32559333
DOI
10.1111/febs.15457
Knihovny.cz E-zdroje
- MeSH
- bakteriální proteiny chemie metabolismus MeSH
- cukry metabolismus MeSH
- gramnegativní bakteriální infekce imunologie metabolismus mikrobiologie MeSH
- hemocyty imunologie metabolismus MeSH
- hemolymfa imunologie metabolismus MeSH
- imunita imunologie MeSH
- imunitní systém imunologie metabolismus MeSH
- interakce hostitele a patogenu imunologie MeSH
- lektiny chemie metabolismus MeSH
- lidé MeSH
- metylace MeSH
- můry MeSH
- Photorhabdus imunologie metabolismus fyziologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
O-methylation is an unusual sugar modification with a function that is not fully understood. Given its occurrence and recognition by lectins involved in the immune response, methylated sugars were proposed to represent a conserved pathogen-associated molecular pattern. We describe the interaction of O-methylated saccharides with two β-propeller lectins, the newly described PLL2 from the entomopathogenic bacterium Photorhabdus laumondii, and its homologue PHL from the related human pathogen Photorhabdus asymbiotica. The crystal structures of PLL2 and PHL revealed up to 10 out of 14 potential binding sites per protein subunit to be occupied with O-methylated structures. The avidity effect strengthens the interaction by 4 orders of magnitude. PLL2 and PHL also interfere with the early immune response by modulating the production of reactive oxygen species and phenoloxidase activity. Since bacteria from Photorhabdus spp. have a complex life cycle involving pathogenicity towards different hosts, the involvement of PLL2 and PHL might contribute to the pathogen overcoming insect and human immune system defences in the early stages of infection. DATABASES: Structural data are available in PDB database under the accession numbers 6RG2, 6RGG, 6RFZ, 6RG1, 6RGU, 6RGW, 6RGJ, and 6RGR.
Central European Institute of Technology Masaryk University Brno Czech Republic
Department of Biochemistry Faculty of Science Masaryk University Brno Czech Republic
N D Zelinsky Institute of Organic Chemistry Russian Academy of Sciences Moscow Russia
National Centre for Biomolecular Research Faculty of Science Masaryk University Brno Czech Republic
Citace poskytuje Crossref.org
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