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Analytical techniques for multiplex analysis of protein biomarkers
A. Van Gool, F. Corrales, M. Čolović, D. Krstić, B. Oliver-Martos, E. Martínez-Cáceres, I. Jakasa, G. Gajski, V. Brun, K. Kyriacou, I. Burzynska-Pedziwiatr, LA. Wozniak, S. Nierkens, C. Pascual García, J. Katrlik, Z. Bojic-Trbojevic, J. Vacek, A....
Language English Country Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
- MeSH
- Biomarkers analysis chemistry MeSH
- Mass Spectrometry methods MeSH
- Immunoassay methods MeSH
- Humans MeSH
- Proteomics methods MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
INTRODUCTION: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. AREAS COVERED: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. EXPERT COMMENTARY: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.
Center for Primary Care and Public Health University of Lausanne Lausanne Switzerland
Department of Chemistry Graduate School of Sciences and Engineering Koç University Istanbul Turkey
Department of Neurology Erasmus MC Rotterdam The Netherlands
Ephraim Katzir Department of Biotechnology Engineering ORT Braude College Karmiel Israel
Functional Proteomics Laboratory Centro Nacional De Biotecnología Madrid Spain
Immunologix Laboratories Tampa FL USA
Institute of Cancer Therapeutics University of Bradford Bradford UK
Institute of Medical Chemistry Faculty of Medicine University of Belgrade Belgrade Serbia
Institute of Natural Sciences Department of Biochemistry Ege University Izmir Turkey
Medisiina D 6 A B University of Turku Turku Finland
Mutagenesis Unit Institute for Medical Research and Occupational Health Zagreb Croatia
Research Institute for Chromatography Kortrijk Belgium
Université Grenoble Alpes CEA Inserm IRIG BGE Grenoble France
References provided by Crossref.org
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