• Something wrong with this record ?

Preparation of PLGA microspheres loaded with 10-hydroxycamptothecin and arsenic trioxide and their treatment for rabbit hepatocellular carcinoma

Y. Wu, D. Du, J. Chen, C. Liu

. 2021 ; 165 (1) : 57-63. [pub] 20200106

Language English Country Czech Republic

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21028027

OBJECTIVE: This study aims to study the preparation method of arsenic trioxide (As2O3) polylactic-co-glyconlic acid (PLGA) microspheres and 10-hydroxycamptothecin (HCPT) PLGA microspheres and explore their therapeutic effects as embolic agents for VX2 hepatocellular carcinoma in rabbits. METHODS: As2O3 and HCPT PLGA microspheres were prepared by multiple emulsion solvent evaporation method. Scanning electron microscopy (SEM) and particle size distribution were used to analyze the morphology, the drug sustained release ability was observed by the release of microspheres in vitro. The rabbit model of VX2 hepatocellular carcinoma was established and the hepatocellular carcinoma was treated with combined microspheres. The therapeutic effects were detected by qPCR, western blotting, HE staining and immunohistochemical methods. RESULTS: The PLGA microspheres loaded with As2O3 and HCPT were successfully prepared by optimizing the ratio. The particle size was between 30 and 50 μm. In vitro release results showed that PLGA microspheres loaded with As2O3 released completely in 10 days and PLGA microspheres loaded with HCPT released completely in 12 days. Western blotting and qPCR results showed that the expression of ALDH1A1 and Nanog decreased significantly in treatment group. HE staining and immunohistochemical analysis showed that the expression of CD31, HIF and VEGF decreased significantly and the apoptosis of tissues was obvious. CONCLUSION: The combination of As2O3 and HCPT PLGA microspheres as embolization for VX2 hepatocellular carcinoma in rabbits has significant therapeutic effect.

References provided by Crossref.org

Bibliography, etc.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc21028027
003      
CZ-PrNML
005      
20211129142954.0
007      
ta
008      
211105s2021 xr ad f 000 0|eng||
009      
AR
024    7_
$a 10.5507/bp.2019.063 $2 doi
035    __
$a (PubMed)31907490
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Wu, Yumin $u Department of Interventional Therapy, Shenzhen Second People's Hospital. No. 3002 Sungang West Road, Futian District, Shenzhen, Guangdong 518035, P.R.China
245    10
$a Preparation of PLGA microspheres loaded with 10-hydroxycamptothecin and arsenic trioxide and their treatment for rabbit hepatocellular carcinoma / $c Y. Wu, D. Du, J. Chen, C. Liu
504    __
$a Literatura
520    9_
$a OBJECTIVE: This study aims to study the preparation method of arsenic trioxide (As2O3) polylactic-co-glyconlic acid (PLGA) microspheres and 10-hydroxycamptothecin (HCPT) PLGA microspheres and explore their therapeutic effects as embolic agents for VX2 hepatocellular carcinoma in rabbits. METHODS: As2O3 and HCPT PLGA microspheres were prepared by multiple emulsion solvent evaporation method. Scanning electron microscopy (SEM) and particle size distribution were used to analyze the morphology, the drug sustained release ability was observed by the release of microspheres in vitro. The rabbit model of VX2 hepatocellular carcinoma was established and the hepatocellular carcinoma was treated with combined microspheres. The therapeutic effects were detected by qPCR, western blotting, HE staining and immunohistochemical methods. RESULTS: The PLGA microspheres loaded with As2O3 and HCPT were successfully prepared by optimizing the ratio. The particle size was between 30 and 50 μm. In vitro release results showed that PLGA microspheres loaded with As2O3 released completely in 10 days and PLGA microspheres loaded with HCPT released completely in 12 days. Western blotting and qPCR results showed that the expression of ALDH1A1 and Nanog decreased significantly in treatment group. HE staining and immunohistochemical analysis showed that the expression of CD31, HIF and VEGF decreased significantly and the apoptosis of tissues was obvious. CONCLUSION: The combination of As2O3 and HCPT PLGA microspheres as embolization for VX2 hepatocellular carcinoma in rabbits has significant therapeutic effect.
650    _2
$a zvířata $7 D000818
650    _2
$a protinádorové látky $x aplikace a dávkování $7 D000970
650    _2
$a oxid arsenitý $x aplikace a dávkování $7 D000077237
650    _2
$a kamptothecin $x aplikace a dávkování $x analogy a deriváty $7 D002166
650    _2
$a hepatocelulární karcinom $x farmakoterapie $7 D006528
650    _2
$a modely nemocí na zvířatech $7 D004195
650    _2
$a nádory jater $x farmakoterapie $7 D008113
650    12
$a mikrosféry $7 D008863
650    12
$a kopolymer kyseliny glykolové a mléčné $7 D000077182
650    _2
$a králíci $7 D011817
655    _2
$a časopisecké články $7 D016428
700    1_
$a Du, Duanming $u Department of Interventional Therapy, Shenzhen Second People's Hospital. No. 3002 Sungang West Road, Futian District, Shenzhen, Guangdong 518035, P.R.China
700    1_
$a Chen, Juanping $u Department of Interventional Therapy, Shenzhen Second People's Hospital. No. 3002 Sungang West Road, Futian District, Shenzhen, Guangdong 518035, P.R.China
700    1_
$a Liu, Chunlin $u Department of Interventional Therapy, Shenzhen Second People's Hospital. No. 3002 Sungang West Road, Futian District, Shenzhen, Guangdong 518035, P.R.China
773    0_
$w MED00012606 $t Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia $x 1213-8118 $g Roč. 165, č. 1 (2021), s. 57-63
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31907490 $y Pubmed
910    __
$a ABA008 $b A 1502 $c 958 $y p $z 0
990    __
$a 20211105 $b ABA008
991    __
$a 20211116104205 $b ABA008
999    __
$a ok $b bmc $g 1728689 $s 1148572
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 165 $c 1 $d 57-63 $e 20200106 $i 1213-8118 $m Biomedical papers of the Medical Faculty of the University Palacký, Olomouc Czech Republic $n Biomed. Pap. Fac. Med. Palacký Univ. Olomouc Czech Repub. (Print) $x MED00012606
LZP    __
$b NLK118 $a Pubmed-20211105

Find record

Citation metrics

Archiving options