-
Je něco špatně v tomto záznamu ?
Myo-Inositol Levels in the Dorsal Hippocampus Serve as Glial Prognostic Marker of Mild Cognitive Impairment in Mice
T. Ebert, DE. Heinz, S. Almeida-Corrêa, R. Cruz, F. Dethloff, T. Stark, T. Bajaj, OM. Maurel, FM. Ribeiro, S. Calcagnini, K. Hafner, NC. Gassen, CW. Turck, B. Boulat, M. Czisch, CT. Wotjak
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
NLK
Directory of Open Access Journals
od 2009
Free Medical Journals
od 2009
PubMed Central
od 2009
Europe PubMed Central
od 2009
ProQuest Central
od 2009-07-30
Open Access Digital Library
od 2009-01-01
Open Access Digital Library
od 2009-01-01
Health & Medicine (ProQuest)
od 2009-07-30
ROAD: Directory of Open Access Scholarly Resources
od 2009
- Publikační typ
- časopisecké články MeSH
Dementia is a devastating age-related disorder. Its therapy would largely benefit from the identification of susceptible subjects at early, prodromal stages of the disease. To search for such prognostic markers of cognitive impairment, we studied spatial navigation in male BALBc vs. B6N mice in combination with in vivo magnetic resonance spectroscopy (1H-MRS). BALBc mice consistently showed higher escape latencies than B6N mice, both in the Water Cross Maze (WCM) and the Morris water maze (MWM). These performance deficits coincided with higher levels of myo-inositol (mIns) in the dorsal hippocampus before and after training. Subsequent biochemical analyses of hippocampal specimens by capillary immunodetection and liquid chromatography mass spectrometry-based (LC/MS) metabolomics revealed a higher abundance of glial markers (IBA-1, S100B, and GFAP) as well as distinct alterations in metabolites including a decrease in vitamins (pantothenic acid and nicotinamide), neurotransmitters (acetylcholine), their metabolites (glutamine), and acetyl-L-carnitine. Supplementation of low abundant acetyl-L-carnitine via the drinking water, however, failed to revert the behavioral deficits shown by BALBc mice. Based on our data we suggest (i) BALBc mice as an animal model and (ii) hippocampal mIns levels as a prognostic marker of mild cognitive impairment (MCI), due to (iii) local changes in microglia and astrocyte activity, which may (iv) result in decreased concentrations of promnesic molecules.
Department of Pharmacology Faculty of Medicine Masaryk University Brno Czechia
Department of Translational Research in Psychiatry Max Planck Institute of Psychiatry Munich Germany
Max Planck School of Cognition Leipzig Germany
Proteomics and Biomarkers Max Planck Institute of Psychiatry Munich Germany
Research Group Neuronal Plasticity Max Planck Institute of Psychiatry Munich Germany
Scientific Core Unit Neuroimaging Max Planck Institute of Psychiatry Munich Germany
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22001312
- 003
- CZ-PrNML
- 005
- 20220112153546.0
- 007
- ta
- 008
- 220107s2021 sz f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.3389/fnagi.2021.731603 $2 doi
- 035 __
- $a (PubMed)34867270
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a sz
- 100 1_
- $a Ebert, Tim $u Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, Munich, Germany $u Research Group Neurohomeostasis, Department of Psychiatry and Psychotherapy, University Hospital Bonn, Bonn, Germany
- 245 10
- $a Myo-Inositol Levels in the Dorsal Hippocampus Serve as Glial Prognostic Marker of Mild Cognitive Impairment in Mice / $c T. Ebert, DE. Heinz, S. Almeida-Corrêa, R. Cruz, F. Dethloff, T. Stark, T. Bajaj, OM. Maurel, FM. Ribeiro, S. Calcagnini, K. Hafner, NC. Gassen, CW. Turck, B. Boulat, M. Czisch, CT. Wotjak
- 520 9_
- $a Dementia is a devastating age-related disorder. Its therapy would largely benefit from the identification of susceptible subjects at early, prodromal stages of the disease. To search for such prognostic markers of cognitive impairment, we studied spatial navigation in male BALBc vs. B6N mice in combination with in vivo magnetic resonance spectroscopy (1H-MRS). BALBc mice consistently showed higher escape latencies than B6N mice, both in the Water Cross Maze (WCM) and the Morris water maze (MWM). These performance deficits coincided with higher levels of myo-inositol (mIns) in the dorsal hippocampus before and after training. Subsequent biochemical analyses of hippocampal specimens by capillary immunodetection and liquid chromatography mass spectrometry-based (LC/MS) metabolomics revealed a higher abundance of glial markers (IBA-1, S100B, and GFAP) as well as distinct alterations in metabolites including a decrease in vitamins (pantothenic acid and nicotinamide), neurotransmitters (acetylcholine), their metabolites (glutamine), and acetyl-L-carnitine. Supplementation of low abundant acetyl-L-carnitine via the drinking water, however, failed to revert the behavioral deficits shown by BALBc mice. Based on our data we suggest (i) BALBc mice as an animal model and (ii) hippocampal mIns levels as a prognostic marker of mild cognitive impairment (MCI), due to (iii) local changes in microglia and astrocyte activity, which may (iv) result in decreased concentrations of promnesic molecules.
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Heinz, Daniel E $u Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, Munich, Germany $u Max Planck School of Cognition, Leipzig, Germany
- 700 1_
- $a Almeida-Corrêa, Suellen $u Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Cruz, Renata $u Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Dethloff, Frederik $u Proteomics and Biomarkers, Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Stark, Tibor $u Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, Munich, Germany $u Department of Pharmacology, Faculty of Medicine, Masaryk University, Brno, Czechia $u Scientific Core Unit "Neuroimaging", Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Bajaj, Thomas $u Research Group Neurohomeostasis, Department of Psychiatry and Psychotherapy, University Hospital Bonn, Bonn, Germany
- 700 1_
- $a Maurel, Oriana M $u Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Ribeiro, Fabiola M $u Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Calcagnini, Silvio $u Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Hafner, Kathrin $u Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Gassen, Nils C $u Research Group Neurohomeostasis, Department of Psychiatry and Psychotherapy, University Hospital Bonn, Bonn, Germany $u Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Turck, Christoph W $u Proteomics and Biomarkers, Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Boulat, Benoit $u Scientific Core Unit "Neuroimaging", Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Czisch, Michael $u Scientific Core Unit "Neuroimaging", Max Planck Institute of Psychiatry, Munich, Germany
- 700 1_
- $a Wotjak, Carsten T $u Research Group Neuronal Plasticity, Max Planck Institute of Psychiatry, Munich, Germany $u Central Nervous System Diseases Research (CNSDR), Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riss, Germany
- 773 0_
- $w MED00174539 $t Frontiers in aging neuroscience $x 1663-4365 $g Roč. 13, č. - (2021), s. 731603
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/34867270 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20220107 $b ABA008
- 991 __
- $a 20220112153542 $b ABA008
- 999 __
- $a ind $b bmc $g 1745434 $s 1152459
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 13 $c - $d 731603 $e 20211112 $i 1663-4365 $m Frontiers in aging neuroscience $n Front Aging Neurosci $x MED00174539
- LZP __
- $a Pubmed-20220107