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Species-specific PCR assay for the detection of Babesia odocoilei

HJ. Burgess, BP. Lockerbie, LE. Ayalew, A. Dibernardo, K. Hrazdilová, D. Modry, TK. Bollinger

. 2021 ; 33 (6) : 1188-1192. [pub] 20210922

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22003337

We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.

Citace poskytuje Crossref.org

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