• Je něco špatně v tomto záznamu ?

Naturally zinc-containing bacteriochlorophyll a ([Zn]-BChl a) protects the photosynthetic apparatus of Acidiphilium rubrum from copper toxicity damage

N. Jaime-Pérez, D. Bína, D. Kaftan, SNH. Bokhari, H. Küpper

. 2021 ; 1862 (10) : 148472. [pub] 20210702

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22003530

In almost all photosynthetic organisms the photosynthetic pigments chlorophyll and bacteriochlorophyll (BChl) are Mg2+ containing complexes, but Mg2+ may be exchanged against other metal ions when these are present in toxic concentrations, leading to inactivation of photosynthesis. In this report we studied mechanisms of copper toxicity to the photosynthetic apparatus of Acidiphilium rubrum, an acidophilic purple bacterium that uses Zn2+ instead of Mg2+ as the central metal in the BChl molecules ([Zn]-BChl) of its reaction centres (RCs) and light harvesting proteins (LH1). We used a combination of in vivo measurements of photosynthetic activity (fast fluorescence and absorption kinetics) together with analysis of metal binding to pigments and pigment-protein complexes by HPLC-ICP-sfMS to monitor the effect of Cu2+ on photosynthesis of A. rubrum. Further, we found that its cytoplasmic pH is neutral. We compared these results with those obtained from Rhodospirillum rubrum, a purple bacterium for which we previously reported that the central Mg2+ of BChl can be replaced in vivo in the RCs by Cu2+ under environmentally realistic Cu2+ concentrations, leading to a strong inhibition of photosynthesis. Thus, we observed that A. rubrum is much more resistant to copper toxicity than R. rubrum. Only slight changes of photosynthetic parameters were observed in A. rubrum at copper concentrations that were severely inhibitory in R. rubrum and in A. rubrum no copper complexes of BChl were found. Altogether, the data suggest that [Zn]-BChl protects the photosynthetic apparatus of A. rubrum from detrimental insertion of Cu2+ (trans-metallation) into BChl molecules of its RCs.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22003530
003      
CZ-PrNML
005      
20220127150130.0
007      
ta
008      
220113s2021 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.bbabio.2021.148472 $2 doi
035    __
$a (PubMed)34217700
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Jaime-Pérez, Noelia $u Czech Academy of Sciences, Biology Centre, Institute of Plant Molecular Biology, Department of Plant Biophysics and Biochemistry, Branišovská 31/1160, 370 05 České Budějovice, Czech Republic
245    10
$a Naturally zinc-containing bacteriochlorophyll a ([Zn]-BChl a) protects the photosynthetic apparatus of Acidiphilium rubrum from copper toxicity damage / $c N. Jaime-Pérez, D. Bína, D. Kaftan, SNH. Bokhari, H. Küpper
520    9_
$a In almost all photosynthetic organisms the photosynthetic pigments chlorophyll and bacteriochlorophyll (BChl) are Mg2+ containing complexes, but Mg2+ may be exchanged against other metal ions when these are present in toxic concentrations, leading to inactivation of photosynthesis. In this report we studied mechanisms of copper toxicity to the photosynthetic apparatus of Acidiphilium rubrum, an acidophilic purple bacterium that uses Zn2+ instead of Mg2+ as the central metal in the BChl molecules ([Zn]-BChl) of its reaction centres (RCs) and light harvesting proteins (LH1). We used a combination of in vivo measurements of photosynthetic activity (fast fluorescence and absorption kinetics) together with analysis of metal binding to pigments and pigment-protein complexes by HPLC-ICP-sfMS to monitor the effect of Cu2+ on photosynthesis of A. rubrum. Further, we found that its cytoplasmic pH is neutral. We compared these results with those obtained from Rhodospirillum rubrum, a purple bacterium for which we previously reported that the central Mg2+ of BChl can be replaced in vivo in the RCs by Cu2+ under environmentally realistic Cu2+ concentrations, leading to a strong inhibition of photosynthesis. Thus, we observed that A. rubrum is much more resistant to copper toxicity than R. rubrum. Only slight changes of photosynthetic parameters were observed in A. rubrum at copper concentrations that were severely inhibitory in R. rubrum and in A. rubrum no copper complexes of BChl were found. Altogether, the data suggest that [Zn]-BChl protects the photosynthetic apparatus of A. rubrum from detrimental insertion of Cu2+ (trans-metallation) into BChl molecules of its RCs.
650    _2
$a Acidiphilium $x chemie $7 D041801
650    _2
$a bakteriochlorofyl A $x chemie $7 D025541
650    _2
$a vysokoúčinná kapalinová chromatografie $7 D002851
650    _2
$a měď $x chemie $x toxicita $7 D003300
650    _2
$a koncentrace vodíkových iontů $7 D006863
650    _2
$a světlosběrné proteinové komplexy $x chemie $7 D045342
650    _2
$a hořčík $x chemie $7 D008274
650    _2
$a hmotnostní spektrometrie $7 D013058
650    _2
$a fotosyntéza $7 D010788
650    _2
$a Rhodospirillum rubrum $x chemie $7 D012247
650    _2
$a vztahy mezi strukturou a aktivitou $7 D013329
650    _2
$a zinek $x chemie $7 D015032
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Bína, David $u University of South Bohemia, Department of Chemistry, Branišovská 1760, 370 05 České Budějovice, Czech Republic; Czech Academy of Sciences, Biology Centre, Institute of Plant Molecular Biology, Department of Photosynthesis, Branišovská 31/1160, 370 05 České Budějovice, Czech Republic
700    1_
$a Kaftan, David $u University of South Bohemia, Department of Chemistry, Branišovská 1760, 370 05 České Budějovice, Czech Republic; Czech Academy of Sciences, Institute of Microbiology, Centre Algatech, Novohradská 237, 37981 Třeboň, Czech Republic
700    1_
$a Bokhari, Syed Nadeem Hussain $u Czech Academy of Sciences, Biology Centre, Institute of Plant Molecular Biology, Department of Plant Biophysics and Biochemistry, Branišovská 31/1160, 370 05 České Budějovice, Czech Republic
700    1_
$a Küpper, Hendrik $u Czech Academy of Sciences, Biology Centre, Institute of Plant Molecular Biology, Department of Plant Biophysics and Biochemistry, Branišovská 31/1160, 370 05 České Budějovice, Czech Republic; University of South Bohemia, Department of Experimental Plant Biology, Branišovská 31/1160, 370 05 České Budějovice, Czech Republic. Electronic address: hendrik.kuepper@umbr.cas.cz
773    0_
$w MED00000712 $t Biochimica et biophysica acta. Bioenergetics $x 1879-2650 $g Roč. 1862, č. 10 (2021), s. 148472
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34217700 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220113 $b ABA008
991    __
$a 20220127150126 $b ABA008
999    __
$a ok $b bmc $g 1751092 $s 1154679
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 1862 $c 10 $d 148472 $e 20210702 $i 1879-2650 $m Biochimica et biophysica acta. Bioenergetics $n Biochem Biophys Acta $x MED00000712
LZP    __
$a Pubmed-20220113
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace