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Differences in mitochondrial NADH dehydrogenase activities in trypanosomatids

P. Čermáková, A. Maďarová, P. Baráth, J. Bellová, V. Yurchenko, A. Horváth

. 2021 ; 148 (10) : 1161-1170. [pub] 20210107

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc22003856

Complex I (NADH dehydrogenase) is the first enzyme in the respiratory chain. It catalyses the electron transfer from NADH to ubiquinone that is associated with proton pumping out of the matrix. In this study, we characterized NADH dehydrogenase activity in seven monoxenous trypanosomatid species: Blechomonas ayalai, Herpetomonas tarakana, Kentomonas sorsogonicus, Leptomonas seymouri, Novymonas esmeraldas, Sergeia podlipaevi and Wallacemonas raviniae. We also investigated the subunit composition of the complex I in dixenous Phytomonas serpens, in which its presence and activity have been previously documented. In addition to P. serpens, the complex I is functionally active in N. esmeraldas and S. podlipaevi. We also identified 24-32 subunits of the complex I in individual species by using mass spectrometry. Among them, for the first time, we recognized several proteins of the mitochondrial DNA origin.

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$a Maďarová, Anna $u Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia
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$a Baráth, Peter $u Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia
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