Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Blastocystis in the faeces of children from six distant countries: prevalence, quantity, subtypes and the relation to the gut bacteriome

O. Cinek, K. Polackova, R. Odeh, A. Alassaf, L. Kramná, MU. Ibekwe, ES. Majaliwa, G. Ahmadov, BME. Elmahi, H. Mekki, S. Oikarinen, J. Lebl, MA. Abdullah

. 2021 ; 14 (1) : 399. [pub] 20210812

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22003933

Grantová podpora
15-31426A Ministerstvo Zdravotnictví Ceské Republiky
00064203 Ministerstvo Zdravotnictví Ceské Republiky

BACKGROUND: Blastocystis is a human gut symbiont of yet undefined clinical significance. In a set of faecal samples collected from asymptomatic children of six distant populations, we first assessed the community profiles of protist 18S rDNA and then characterized Blastocystis subtypes and tested Blastocystis association with the faecal bacteriome community. METHODS: Stool samples were collected from 244 children and young persons (mean age 11.3 years, interquartile range 8.1-13.7) of six countries (Azerbaijan 51 subjects, Czechia 52, Jordan 40, Nigeria 27, Sudan 59 and Tanzania 15). The subjects showed no symptoms of infection. Amplicon profiling of the 18S rDNA was used for verification that Blastocystis was the most frequent protist, whereas specific real-time PCR showed its prevalence and quantity, and massive parallel amplicon sequencing defined the Blastocystis subtypes. The relation between Blastocystis and the stool bacteriome community was characterized using 16S rDNA profiling. RESULTS: Blastocystis was detected by specific PCR in 36% (88/244) stool samples and was the most often observed faecal protist. Children from Czechia and Jordan had significantly lower prevalence than children from the remaining countries. The most frequent subtype was ST3 (49%, 40/81 sequenced samples), followed by ST1 (36%) and ST2 (25%). Co-infection with two different subtypes was noted in 12% samples. The faecal bacteriome had higher richness in Blastocystis-positive samples, and Blastocystis was associated with significantly different community composition regardless of the country (p < 0.001 in constrained redundancy analysis). Several taxa differed with Blastocystis positivity or quantity: two genera of Ruminococcaceae were more abundant, while Bifidobacterium, Veillonella, Lactobacillus and several other genera were undrerrepresented. CONCLUSIONS: Asymptomatic children frequently carry Blastocystis, and co-infection with multiple distinct subtypes is not exceptional. Prevalence and quantity of the organism clearly differ among populations. Blastocystis is linked to both faecal bacteriome diversity and its composition.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22003933
003      
CZ-PrNML
005      
20220127145716.0
007      
ta
008      
220113s2021 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1186/s13071-021-04859-3 $2 doi
035    __
$a (PubMed)34384477
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Cinek, Ondrej $u Department of Pediatrics, 2nd Faculty of Medicine, Charles University in Prague and University Hospital Motol, V Uvalu 84, Prague 5, Czech Republic. ondrej.cinek@lfmotol.cuni.cz
245    10
$a Blastocystis in the faeces of children from six distant countries: prevalence, quantity, subtypes and the relation to the gut bacteriome / $c O. Cinek, K. Polackova, R. Odeh, A. Alassaf, L. Kramná, MU. Ibekwe, ES. Majaliwa, G. Ahmadov, BME. Elmahi, H. Mekki, S. Oikarinen, J. Lebl, MA. Abdullah
520    9_
$a BACKGROUND: Blastocystis is a human gut symbiont of yet undefined clinical significance. In a set of faecal samples collected from asymptomatic children of six distant populations, we first assessed the community profiles of protist 18S rDNA and then characterized Blastocystis subtypes and tested Blastocystis association with the faecal bacteriome community. METHODS: Stool samples were collected from 244 children and young persons (mean age 11.3 years, interquartile range 8.1-13.7) of six countries (Azerbaijan 51 subjects, Czechia 52, Jordan 40, Nigeria 27, Sudan 59 and Tanzania 15). The subjects showed no symptoms of infection. Amplicon profiling of the 18S rDNA was used for verification that Blastocystis was the most frequent protist, whereas specific real-time PCR showed its prevalence and quantity, and massive parallel amplicon sequencing defined the Blastocystis subtypes. The relation between Blastocystis and the stool bacteriome community was characterized using 16S rDNA profiling. RESULTS: Blastocystis was detected by specific PCR in 36% (88/244) stool samples and was the most often observed faecal protist. Children from Czechia and Jordan had significantly lower prevalence than children from the remaining countries. The most frequent subtype was ST3 (49%, 40/81 sequenced samples), followed by ST1 (36%) and ST2 (25%). Co-infection with two different subtypes was noted in 12% samples. The faecal bacteriome had higher richness in Blastocystis-positive samples, and Blastocystis was associated with significantly different community composition regardless of the country (p < 0.001 in constrained redundancy analysis). Several taxa differed with Blastocystis positivity or quantity: two genera of Ruminococcaceae were more abundant, while Bifidobacterium, Veillonella, Lactobacillus and several other genera were undrerrepresented. CONCLUSIONS: Asymptomatic children frequently carry Blastocystis, and co-infection with multiple distinct subtypes is not exceptional. Prevalence and quantity of the organism clearly differ among populations. Blastocystis is linked to both faecal bacteriome diversity and its composition.
650    _2
$a mladiství $7 D000293
650    _2
$a asymptomatické infekce $x epidemiologie $7 D058345
650    _2
$a Blastocystis $x klasifikace $x genetika $x izolace a purifikace $7 D016844
650    _2
$a blastocystóza $x epidemiologie $x parazitologie $7 D016776
650    _2
$a dítě $7 D002648
650    _2
$a protozoální DNA $x genetika $7 D016054
650    _2
$a ribozomální DNA $x genetika $7 D004275
650    _2
$a feces $x parazitologie $7 D005243
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a střevní mikroflóra $x genetika $7 D000069196
650    _2
$a genetická variace $7 D014644
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a prevalence $7 D015995
651    _2
$a Ázerbájdžán $x epidemiologie $7 D001382
651    _2
$a Československo $x epidemiologie $7 D003604
651    _2
$a Jordánsko $x epidemiologie $7 D007597
651    _2
$a Nigérie $x epidemiologie $7 D009549
651    _2
$a Súdán $x epidemiologie $7 D013397
651    _2
$a Tanzanie $x epidemiologie $7 D013636
655    _2
$a časopisecké články $7 D016428
700    1_
$a Polackova, Katerina $u Department of Pediatrics, 2nd Faculty of Medicine, Charles University in Prague and University Hospital Motol, V Uvalu 84, Prague 5, Czech Republic
700    1_
$a Odeh, Rasha $u Department of Pediatrics, School of Medicine, University of Jordan, Amman, Jordan
700    1_
$a Alassaf, Abeer $u Department of Pediatrics, School of Medicine, University of Jordan, Amman, Jordan
700    1_
$a Kramná, Lenka $u Department of Pediatrics, 2nd Faculty of Medicine, Charles University in Prague and University Hospital Motol, V Uvalu 84, Prague 5, Czech Republic
700    1_
$a Ibekwe, MaryAnn Ugochi $u Department of Pediatrics, Federal Teaching Hospital Abakaliki, Ebonyi State University, Abakaliki, Nigeria
700    1_
$a Majaliwa, Edna Siima $u Muhimbili National Hospital, Dar-es-Salaam, Tanzania
700    1_
$a Ahmadov, Gunduz $u Endocrine Centre Baku, Str. I. Hashimov 4A, AZ1114, Baku, Azerbaijan
700    1_
$a Elmahi, Bashir Mukhtar Elwasila $u Department of Paediatrics and Child Health, University of Khartoum, Faculty of Medicine, Khartoum, Sudan $u Sudan Childhood Diabetes Center, Khartoum, Sudan
700    1_
$a Mekki, Hanan $u Department of Paediatrics and Child Health, University of Khartoum, Faculty of Medicine, Khartoum, Sudan
700    1_
$a Oikarinen, Sami $u Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland
700    1_
$a Lebl, Jan $u Department of Pediatrics, 2nd Faculty of Medicine, Charles University in Prague and University Hospital Motol, V Uvalu 84, Prague 5, Czech Republic
700    1_
$a Abdullah, Mohammed Ahmed $u Department of Paediatrics and Child Health, University of Khartoum, Faculty of Medicine, Khartoum, Sudan $u Sudan Childhood Diabetes Center, Khartoum, Sudan
773    0_
$w MED00165371 $t Parasites & vectors $x 1756-3305 $g Roč. 14, č. 1 (2021), s. 399
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34384477 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220113 $b ABA008
991    __
$a 20220127145713 $b ABA008
999    __
$a ok $b bmc $g 1751408 $s 1155082
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 14 $c 1 $d 399 $e 20210812 $i 1756-3305 $m Parasites & vectors $n Parasit Vectors $x MED00165371
GRA    __
$a 15-31426A $p Ministerstvo Zdravotnictví Ceské Republiky
GRA    __
$a 00064203 $p Ministerstvo Zdravotnictví Ceské Republiky
LZP    __
$a Pubmed-20220113

Najít záznam

Citační ukazatele

Možnosti archivace