Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Transcriptome sequencing and multi-plex imaging of prostate cancer microenvironment reveals a dominant role for monocytic cells in progression

S. Mangiola, P. McCoy, M. Modrak, F. Souza-Fonseca-Guimaraes, D. Blashki, R. Stuchbery, SP. Keam, M. Kerger, K. Chow, C. Nasa, M. Le Page, N. Lister, S. Monard, J. Peters, P. Dundee, SG. Williams, AJ. Costello, PJ. Neeson, B. Pal, ND. Huntington,...

. 2021 ; 21 (1) : 846. [pub] 20210722

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22004064

Grantová podpora
1054618 National Health and Medical Research Council

BACKGROUND: Prostate cancer is caused by genomic aberrations in normal epithelial cells, however clinical translation of findings from analyses of cancer cells alone has been very limited. A deeper understanding of the tumour microenvironment is needed to identify the key drivers of disease progression and reveal novel therapeutic opportunities. RESULTS: In this study, the experimental enrichment of selected cell-types, the development of a Bayesian inference model for continuous differential transcript abundance, and multiplex immunohistochemistry permitted us to define the transcriptional landscape of the prostate cancer microenvironment along the disease progression axis. An important role of monocytes and macrophages in prostate cancer progression and disease recurrence was uncovered, supported by both transcriptional landscape findings and by differential tissue composition analyses. These findings were corroborated and validated by spatial analyses at the single-cell level using multiplex immunohistochemistry. CONCLUSIONS: This study advances our knowledge concerning the role of monocyte-derived recruitment in primary prostate cancer, and supports their key role in disease progression, patient survival and prostate microenvironment immune modulation.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22004064
003      
CZ-PrNML
005      
20240206092824.0
007      
ta
008      
220113s2021 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1186/s12885-021-08529-6 $2 doi
035    __
$a (PubMed)34294073
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Mangiola, Stefano $u Bioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia $u Department of Surgery, The University of Melbourne, Parkville, Victoria, Australia $u Department of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia $u Department of Medical Biology, University of Melbourne, Melbourne, Victoria, Australia
245    10
$a Transcriptome sequencing and multi-plex imaging of prostate cancer microenvironment reveals a dominant role for monocytic cells in progression / $c S. Mangiola, P. McCoy, M. Modrak, F. Souza-Fonseca-Guimaraes, D. Blashki, R. Stuchbery, SP. Keam, M. Kerger, K. Chow, C. Nasa, M. Le Page, N. Lister, S. Monard, J. Peters, P. Dundee, SG. Williams, AJ. Costello, PJ. Neeson, B. Pal, ND. Huntington, NM. Corcoran, AT. Papenfuss, CM. Hovens
520    9_
$a BACKGROUND: Prostate cancer is caused by genomic aberrations in normal epithelial cells, however clinical translation of findings from analyses of cancer cells alone has been very limited. A deeper understanding of the tumour microenvironment is needed to identify the key drivers of disease progression and reveal novel therapeutic opportunities. RESULTS: In this study, the experimental enrichment of selected cell-types, the development of a Bayesian inference model for continuous differential transcript abundance, and multiplex immunohistochemistry permitted us to define the transcriptional landscape of the prostate cancer microenvironment along the disease progression axis. An important role of monocytes and macrophages in prostate cancer progression and disease recurrence was uncovered, supported by both transcriptional landscape findings and by differential tissue composition analyses. These findings were corroborated and validated by spatial analyses at the single-cell level using multiplex immunohistochemistry. CONCLUSIONS: This study advances our knowledge concerning the role of monocyte-derived recruitment in primary prostate cancer, and supports their key role in disease progression, patient survival and prostate microenvironment immune modulation.
650    _2
$a výpočetní biologie $x metody $7 D019295
650    _2
$a progrese nemoci $7 D018450
650    12
$a stanovení celkové genové exprese $x metody $7 D020869
650    _2
$a vysoce účinné nukleotidové sekvenování $7 D059014
650    _2
$a lidé $7 D006801
650    _2
$a imunohistochemie $7 D007150
650    _2
$a imunofenotypizace $7 D016130
650    _2
$a Kaplanův-Meierův odhad $7 D053208
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a anotace sekvence $7 D058977
650    _2
$a monocyty $x metabolismus $x patologie $7 D009000
650    _2
$a prognóza $7 D011379
650    _2
$a nádory prostaty $x diagnóza $x genetika $x metabolismus $x mortalita $7 D011471
650    12
$a transkriptom $7 D059467
650    _2
$a nádorové mikroprostředí $x genetika $7 D059016
655    _2
$a časopisecké články $7 D016428
700    1_
$a McCoy, Patrick $u Department of Surgery, The University of Melbourne, Parkville, Victoria, Australia $u Department of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia
700    1_
$a Modrák, Martin $u Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic $7 xx0313530
700    1_
$a Souza-Fonseca-Guimaraes, Fernando $u University of Queensland Diamantina Institute, Translational Research Institute, University of Queensland, Brisbane, QLD, Australia
700    1_
$a Blashki, Daniel $u The Peter Doherty Institute for Infection and Immunity, Parkville, Victoria, Australia
700    1_
$a Stuchbery, Ryan $u Department of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia
700    1_
$a Keam, Simon P $u Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia $u Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australia
700    1_
$a Kerger, Michael $u Department of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia
700    1_
$a Chow, Ken $u Department of Surgery, The University of Melbourne, Parkville, Victoria, Australia $u Department of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia
700    1_
$a Nasa, Chayanica $u Flow Cytometry Facility, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
700    1_
$a Le Page, Melanie $u Flow Cytometry Facility, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
700    1_
$a Lister, Natalie $u Cancer Program, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia $u Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia
700    1_
$a Monard, Simon $u Flow Cytometry Facility, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
700    1_
$a Peters, Justin $u Epworth Center of Cancer Research, Clayton, Victoria, Australia
700    1_
$a Dundee, Phil $u Epworth Center of Cancer Research, Clayton, Victoria, Australia
700    1_
$a Williams, Scott G $u Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia $u Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australia
700    1_
$a Costello, Anthony J $u Department of Surgery, The University of Melbourne, Parkville, Victoria, Australia $u Department of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia
700    1_
$a Neeson, Paul J $u Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia $u Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australia
700    1_
$a Pal, Bhupinder $u The Olivia Newton-John Cancer Research Institute, Heidelberg, Melbourne, Australia
700    1_
$a Huntington, Nicholas D $u Cancer Program, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia $u Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia
700    1_
$a Corcoran, Niall M $u Department of Surgery, The University of Melbourne, Parkville, Victoria, Australia $u Department of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia $u Department of Urology, Frankston Hospital, Frankston, Victoria, Australia
700    1_
$a Papenfuss, Anthony T $u Bioinformatics Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia. papenfuss@wehi.edu.au $u Department of Medical Biology, University of Melbourne, Melbourne, Victoria, Australia. papenfuss@wehi.edu.au $u Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia. papenfuss@wehi.edu.au $u Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australia. papenfuss@wehi.edu.au $u School of Mathematics and Statistics, University of Melbourne, Melbourne, VIC, 3010, Australia. papenfuss@wehi.edu.au
700    1_
$a Hovens, Christopher M $u Department of Surgery, The University of Melbourne, Parkville, Victoria, Australia $u Department of Urology, Royal Melbourne Hospital, Parkville, Victoria, Australia
773    0_
$w MED00008171 $t BMC cancer $x 1471-2407 $g Roč. 21, č. 1 (2021), s. 846
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34294073 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220113 $b ABA008
991    __
$a 20240206092821 $b ABA008
999    __
$a ok $b bmc $g 1751507 $s 1155213
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 21 $c 1 $d 846 $e 20210722 $i 1471-2407 $m BMC cancer $n BMC Cancer $x MED00008171
GRA    __
$a 1054618 $p National Health and Medical Research Council
LZP    __
$a Pubmed-20220113

Najít záznam

Citační ukazatele

Možnosti archivace