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Development of 48-condition buffer screen for protein stability assessment
J. Houser, J. Kosourova, M. Kubickova, M. Wimmerova
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články
Grantová podpora
LM2018127
Ministerstvo Školství, Mládeže a Tělovýchovy
CA15126
Horizon 2020 (COST Action)
- MeSH
- dynamický rozptyl světla MeSH
- fluorometrie MeSH
- proteiny MeSH
- pufry MeSH
- stabilita proteinů * MeSH
- Publikační typ
- časopisecké články MeSH
The determination of a suitable buffer environment for a protein of interest is not an easy task. The requirements of advanced techniques, the demands on the biological material and the researcher time needed for buffer optimization, as well as personal inflexibility, lead frequently to the use of sub-optimal buffers. Here, we demonstrate the design of a 48-condition buffer screen that can be used to determine an appropriate environment for downstream studies. By the combination of several techniques (differential scanning fluorimetry, dynamic light scattering, and bio-layer interferometry), we are able to assess the protein stability, homogeneity and binding activity across the screen with less than half a milligram of protein in 1 day. The application of this screen helps to avoid unsuitable conditions, to explain problems observed upon protein analysis and to choose the most suitable buffers for further research. The screen can be routinely used as a primary screen for buffer optimization in labs and facilities.
Citace poskytuje Crossref.org
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- $a Houser, Josef $u Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic. houser@mail.muni.cz $u National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic. houser@mail.muni.cz
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- $a The determination of a suitable buffer environment for a protein of interest is not an easy task. The requirements of advanced techniques, the demands on the biological material and the researcher time needed for buffer optimization, as well as personal inflexibility, lead frequently to the use of sub-optimal buffers. Here, we demonstrate the design of a 48-condition buffer screen that can be used to determine an appropriate environment for downstream studies. By the combination of several techniques (differential scanning fluorimetry, dynamic light scattering, and bio-layer interferometry), we are able to assess the protein stability, homogeneity and binding activity across the screen with less than half a milligram of protein in 1 day. The application of this screen helps to avoid unsuitable conditions, to explain problems observed upon protein analysis and to choose the most suitable buffers for further research. The screen can be routinely used as a primary screen for buffer optimization in labs and facilities.
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- $a Kubickova, Monika $u Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic
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- $a Wimmerova, Michaela $u Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic $u National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic $u Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic
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