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Charting Extracellular Transcriptomes in The Human Biofluid RNA Atlas
E. Hulstaert, A. Morlion, F. Avila Cobos, K. Verniers, J. Nuytens, E. Vanden Eynde, N. Yigit, J. Anckaert, A. Geerts, P. Hindryckx, P. Jacques, G. Brusselle, KR. Bracke, T. Maes, T. Malfait, T. Derveaux, V. Ninclaus, C. Van Cauwenbergh, K....
Language English Country United States
Document type Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
NLK
Cell Press Free Archives
from 2012
Directory of Open Access Journals
from 2012
Free Medical Journals
from 2012
Freely Accessible Science Journals
from 2012-01-26
Open Access Digital Library
from 2012-01-01
Open Access Digital Library
from 2012-01-26
- MeSH
- Biomarkers * MeSH
- Cohort Studies MeSH
- Humans MeSH
- RNA genetics metabolism MeSH
- Sequence Analysis, RNA methods MeSH
- Gene Expression Profiling methods MeSH
- Body Fluids metabolism MeSH
- Transcriptome * MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, N.I.H., Extramural MeSH
Extracellular RNAs present in biofluids have emerged as potential biomarkers for disease. Where most studies focus on blood-derived fluids, other biofluids may be more informative. We present an atlas of messenger, circular, and small RNA transcriptomes of a comprehensive collection of 20 human biofluids. By means of synthetic spike-in controls, we compare RNA content across biofluids, revealing a 10,000-fold difference in concentration. The circular RNA fraction is increased in most biofluids compared to tissues. Each biofluid transcriptome is enriched for RNA molecules derived from specific tissues and cell types. Our atlas enables an informed selection of the most relevant biofluid to monitor particular diseases. To verify the biomarker potential in these biofluids, four validation cohorts representing a broad spectrum of diseases were profiled, revealing numerous differential RNAs between case and control subjects. Spike-normalized data are publicly available in the R2 web portal for further exploration.
Central European Institute of Technology Masaryk University Brno Czech Republic
Department of Dermatology Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium
Department of Gastroenterology Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium
Department of Neurology Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium
Department of Ophthalmology Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium
Department of Pathology University Hospital Brno Brno Czech Republic
Department of Reproductive Medicine Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium
Department of Respiratory Medicine Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium
Department of Rheumatology Ghent University Hospital C Heymanslaan 10 9000 Ghent Belgium
Masaryk Memorial Cancer Institute Department of Comprehensive Cancer Care Brno Czech Republic
OncoRNALab Cancer Research Institute Ghent Ghent University C Heymanslaan 10 9000 Ghent Belgium
VIB Inflammation Research Center Ghent University Technologiepark 71 9052 Ghent Belgium
References provided by Crossref.org
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