• Something wrong with this record ?

Utility of NKX3.1 immunohistochemistry in the differential diagnosis of seminal vesicles versus prostatic tissue in needle biopsy

T. Pitra, K. Pivovarcikova, R. Alaghehbandan, EM. Compérat, M. Hora, J. Rogala, M. Slisarenko, M. Michal, O. Hes

. 2020 ; 49 (-) : 151644. [pub] 20201017

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22004761

NKX3.1 is considered a reliable immunohistochemical marker of prostatic origin with high specificity and sensitivity. However, NKX3.1 positivity has been described in other neoplastic and non-neoplastic tissues, such as mesenchymal chondrosarcoma, sex-cord stromal tumors, rete testis adenocarcinoma, lobular and ductal carcinoma of the breast, salivary glands, peribronchial submucosal glands, and Sertoli cells. We analyzed expression of two antibodies (mono and polyclonal) of NKX3.1 in a total of 63 non-neoplastic seminal vesicles. We used 52 resection materials (12 seminal vesicles without prostatic adenocarcinoma, 26 seminal vesicles with prostatic adenocarcinoma infiltration, and 14 cases of seminal vesicles infiltrated by urothelial carcinoma) and 11 prostatic core needle biopsies with incidentally sampled fragment of seminal vesicles. In all cases, tissues from seminal vesicles were completely negative for NKX3.1, despite using polyclonal and monoclonal NKX3.1 antibodies, and regardless of the detection system utilized (diaminobenzidine (DAB) versus alkaline phosphatase (AF)). However, prostatic adenocarcinoma was negative in several cases (n = 6), when AF detection system was used. Reaction with DAB was strong and robust in all cases. Based on our data, we can recommend NKX3.1 as a negative immunohistochemical marker of seminal vesicles.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22004761
003      
CZ-PrNML
005      
20220127145014.0
007      
ta
008      
220113s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.anndiagpath.2020.151644 $2 doi
035    __
$a (PubMed)33126153
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Pitra, Tomas $u Department of Urology, Charles University in Prague, Faculty of Medicine in Plzeň, Pilsen, Czech Republic
245    10
$a Utility of NKX3.1 immunohistochemistry in the differential diagnosis of seminal vesicles versus prostatic tissue in needle biopsy / $c T. Pitra, K. Pivovarcikova, R. Alaghehbandan, EM. Compérat, M. Hora, J. Rogala, M. Slisarenko, M. Michal, O. Hes
520    9_
$a NKX3.1 is considered a reliable immunohistochemical marker of prostatic origin with high specificity and sensitivity. However, NKX3.1 positivity has been described in other neoplastic and non-neoplastic tissues, such as mesenchymal chondrosarcoma, sex-cord stromal tumors, rete testis adenocarcinoma, lobular and ductal carcinoma of the breast, salivary glands, peribronchial submucosal glands, and Sertoli cells. We analyzed expression of two antibodies (mono and polyclonal) of NKX3.1 in a total of 63 non-neoplastic seminal vesicles. We used 52 resection materials (12 seminal vesicles without prostatic adenocarcinoma, 26 seminal vesicles with prostatic adenocarcinoma infiltration, and 14 cases of seminal vesicles infiltrated by urothelial carcinoma) and 11 prostatic core needle biopsies with incidentally sampled fragment of seminal vesicles. In all cases, tissues from seminal vesicles were completely negative for NKX3.1, despite using polyclonal and monoclonal NKX3.1 antibodies, and regardless of the detection system utilized (diaminobenzidine (DAB) versus alkaline phosphatase (AF)). However, prostatic adenocarcinoma was negative in several cases (n = 6), when AF detection system was used. Reaction with DAB was strong and robust in all cases. Based on our data, we can recommend NKX3.1 as a negative immunohistochemical marker of seminal vesicles.
650    _2
$a adenokarcinom $x diagnóza $7 D000230
650    _2
$a senioři $7 D000368
650    _2
$a senioři nad 80 let $7 D000369
650    _2
$a nádorové biomarkery $x analýza $x metabolismus $7 D014408
650    _2
$a jehlová biopsie $7 D001707
650    _2
$a diferenciální diagnóza $7 D003937
650    _2
$a homeodoménové proteiny $x analýza $x metabolismus $7 D018398
650    _2
$a lidé $7 D006801
650    _2
$a imunohistochemie $7 D007150
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    _2
$a prostata $x metabolismus $7 D011467
650    _2
$a nádory prostaty $x diagnóza $7 D011471
650    _2
$a semenné váčky $x metabolismus $x patologie $7 D012669
650    _2
$a transkripční faktory $x analýza $x metabolismus $7 D014157
655    _2
$a časopisecké články $7 D016428
700    1_
$a Pivovarcikova, Kristyna $u Department of Pathology, Charles University in Prague, Faculty of Medicine in Plzeň, Pilsen, Czech Republic
700    1_
$a Alaghehbandan, Reza $u Department of Pathology, Faculty of Medicine, University of British Columbia, Royal Columbian Hospital, Vancouver, BC, Canada
700    1_
$a Compérat, Eva Maria $u Department of Pathology, Tenon Hospital, Sorbonne University, France; Department of Pathology, Medical University Vienna, Austria
700    1_
$a Hora, Milan $u Department of Urology, Charles University in Prague, Faculty of Medicine in Plzeň, Pilsen, Czech Republic
700    1_
$a Rogala, Joanna $u Department of Pathology, Charles University in Prague, Faculty of Medicine in Plzeň, Pilsen, Czech Republic
700    1_
$a Slisarenko, Maryna $u Department of Pathology, Charles University in Prague, Faculty of Medicine in Plzeň, Pilsen, Czech Republic
700    1_
$a Michal, Michal $u Department of Pathology, Charles University in Prague, Faculty of Medicine in Plzeň, Pilsen, Czech Republic
700    1_
$a Hes, Ondrej $u Department of Pathology, Charles University in Prague, Faculty of Medicine in Plzeň, Pilsen, Czech Republic. Electronic address: hes@biopticka.cz
773    0_
$w MED00166541 $t Annals of diagnostic pathology $x 1532-8198 $g Roč. 49, č. - (2020), s. 151644
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33126153 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220113 $b ABA008
991    __
$a 20220127145011 $b ABA008
999    __
$a ok $b bmc $g 1752061 $s 1155910
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 49 $c - $d 151644 $e 20201017 $i 1532-8198 $m Annals of diagnostic pathology $n Ann. diagn. pathol. $x MED00166541
LZP    __
$a Pubmed-20220113

Find record

Citation metrics

Archiving options