-
Je něco špatně v tomto záznamu ?
Platelet-rich plasma, platelet-rich fibrin, and enamel matrix derivative for oral mucosal wound healing
J Vokurka, F Hromcik, M Faldyna, E Gopfert, M Vicenova, L Pozarova, Holla L Izakovicova
Jazyk angličtina Země Německo
Grantová podpora
NV16-28462A
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
ProQuest Central od 2009-07-01
Open Access Digital Library od 2010-12-20
Medline Complete (EBSCOhost) od 2008-03-01
Health & Medicine (ProQuest) od 2009-07-01
Odkazy
PubMed
32627981
DOI
10.24425/pjvs.2020.132762
Knihovny.cz E-zdroje
- MeSH
- fibrin bohatý na krevní destičky * MeSH
- hojení ran * účinky léků MeSH
- králíci MeSH
- náhodné rozdělení MeSH
- plazma bohatá na destičky * MeSH
- proteiny zubní skloviny * terapeutické užití MeSH
- ústní sliznice * patologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
Different approaches to enhance healing of hard or soft tissues include the use of cytokines and growth factors to modify cellular behaviour. Numerous growth factors are found in autologous blood concentrates - platelet-rich plasma (PRP) and platelet-rich fibrin (PRF). Enamel matrix derivative (EMD) may improve tissue healing via amelogenins. Bilayered collagen matrix (CM) is used for soft tissue augmentation. The aim of the present study was to assess potential benefits of PRP, PRF and EMD in combination with bilayered collagen matrix or CM alone in treatment of oral mucosal defects in rabbits. Twenty-seven New Zealand white rabbits were included in this randomized controlled trial. Artificial oral mucosal defects were treated with one of these five approaches: PRP+CM, PRF+CM, EMD+CM, CM alone, or left untreated as a negative control - CO. The animals were euthanized 1 day, 7 days, or 28 days after surgery and necropsies were harvested. Histological and molecular biological analyses were performed. All defects were healed by day 28. No differences between PRP+CM, PRF+CM, CM alone and CO groups were recorded at any time point. Slower angiogenesis and a higher presence of inflammatory infiltrate were observed in the EMD+CM group 28 days after surgery. Molecular biological analyses did not reveal any statistically significant changes. In conclusion, no improvement in mucosal healing of wounds covered with a collagen membrane and PRP, PRF, or EMD was observed, compared with CM alone or untreated controls.
Clinic of Dentistry St Anne's University Hospital Pekarska 53 656 91 Brno Czech Republic
Faculty of Medicine Masaryk University Kamenice 753 5 625 00 Brno Czech Republic
Veterinary Research Institute Brno Palackeho tr 1946 1 612 42 Brno Czech Republic
Vokurka J Clinic of Dentistry St Anne's University Hospital Pekarska 53 656 91 Brno Czech Republic
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22008027
- 003
- CZ-PrNML
- 005
- 20220627154330.0
- 007
- ta
- 008
- 220311s2020 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.24425/pjvs.2020.132762 $2 doi
- 035 __
- $a (PubMed)32627981
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Vokurka, Jan $7 xx0214931 $u Vokurka, J. Clinic of Dentistry, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic $u Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic
- 245 10
- $a Platelet-rich plasma, platelet-rich fibrin, and enamel matrix derivative for oral mucosal wound healing / $c J Vokurka, F Hromcik, M Faldyna, E Gopfert, M Vicenova, L Pozarova, Holla L Izakovicova
- 520 9_
- $a Different approaches to enhance healing of hard or soft tissues include the use of cytokines and growth factors to modify cellular behaviour. Numerous growth factors are found in autologous blood concentrates - platelet-rich plasma (PRP) and platelet-rich fibrin (PRF). Enamel matrix derivative (EMD) may improve tissue healing via amelogenins. Bilayered collagen matrix (CM) is used for soft tissue augmentation. The aim of the present study was to assess potential benefits of PRP, PRF and EMD in combination with bilayered collagen matrix or CM alone in treatment of oral mucosal defects in rabbits. Twenty-seven New Zealand white rabbits were included in this randomized controlled trial. Artificial oral mucosal defects were treated with one of these five approaches: PRP+CM, PRF+CM, EMD+CM, CM alone, or left untreated as a negative control - CO. The animals were euthanized 1 day, 7 days, or 28 days after surgery and necropsies were harvested. Histological and molecular biological analyses were performed. All defects were healed by day 28. No differences between PRP+CM, PRF+CM, CM alone and CO groups were recorded at any time point. Slower angiogenesis and a higher presence of inflammatory infiltrate were observed in the EMD+CM group 28 days after surgery. Molecular biological analyses did not reveal any statistically significant changes. In conclusion, no improvement in mucosal healing of wounds covered with a collagen membrane and PRP, PRF, or EMD was observed, compared with CM alone or untreated controls.<ovid:br/><ovid:br/> Copyright© by the Polish Academy of Sciences.
- 650 07
- $a zvířata $7 D000818 $2 czmesh
- 650 17
- $a proteiny zubní skloviny $x terapeutické užití $7 D003746 $2 czmesh
- 650 17
- $a ústní sliznice $x patologie $7 D009061 $2 czmesh
- 650 17
- $a fibrin bohatý na krevní destičky $7 D000073183 $2 czmesh
- 650 17
- $a plazma bohatá na destičky $7 D053657 $2 czmesh
- 650 07
- $a králíci $7 D011817 $2 czmesh
- 650 07
- $a náhodné rozdělení $7 D011897 $2 czmesh
- 650 17
- $a hojení ran $x účinky léků $7 D014945 $2 czmesh
- 700 1_
- $a Hromčík, Filip, $d 1991- $u Clinic of Dentistry, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic $7 xx0271146 $u Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic
- 700 1_
- $a Faldyna, Martin $7 xx0047099 $u Veterinary Research Institute, Brno, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic
- 700 1_
- $a Göpfert, Eduard $7 xx0214933 $u Veterinary Research Institute, Brno, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic
- 700 1_
- $a Vícenová, Monika $7 xx0271278 $u Veterinary Research Institute, Brno, Palackeho tr. 1946/1, 612 42 Brno, Czech Republic
- 700 1_
- $a Pozarova, L. $u Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic $u First Department of Pathological Anatomy, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic
- 700 1_
- $a Izakovičová Hollá, Lydie, $d 1971- $7 nlk19990074300 $u Clinic of Dentistry, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic $u Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic
- 773 0_
- $t Polish Journal of Veterinary Sciences $g Roč. 23, č. 2 (2020), s. 169-176 $p Pol J Vet Sci $x 1505-1773 $w MED00181063
- 773 0_
- $p Pol J Vet Sci $g 23(2):169-176, 2020 Jun
- 910 __
- $a ABA008 $b sig $y p $z 0
- 990 __
- $a 20220311101403 $b ABA008
- 991 __
- $a 20220627154328 $b ABA008
- 999 __
- $a ok $b bmc $g 1768978 $s 1159221
- BAS __
- $a 3
- BMC __
- $a 2020 $b 23 $c 2 $d 169-176 $x MED00181063 $i 1505-1773 $m Polish journal of veterinary sciences
- GRA __
- $a NV16-28462A $p MZ0
- LZP __
- $a 2021-granty