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Deadly Puppy Infection Caused by an MDR Escherichia coli O39 blaCTX-M-15, blaCMY-2, blaDHA-1, and aac(6)-Ib-cr - Positive in a Breeding Kennel in Central Italy
V. Mattioni Marchetti, I. Bitar, A. Mercato, E. Nucleo, F. Marchesini, M. Mancinelli, P. Prati, GS. Scarsi, J. Hrabak, L. Pagani, M. Fabbi, R. Migliavacca
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NV17-29239A
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Free Medical Journals od 2010
PubMed Central od 2010
Europe PubMed Central od 2010
Open Access Digital Library od 2010-01-01
Open Access Digital Library od 2010-01-01
ROAD: Directory of Open Access Scholarly Resources od 2010
Odkazy
PubMed
32351465
DOI
10.3389/fmicb.2020.00584
Knihovny.cz E-zdroje
- MeSH
- bakteriální léková rezistence MeSH
- Escherichia coli genetika patogenita MeSH
- fatální výsledek MeSH
- infekce vyvolané Escherichia coli * mikrobiologie veterinární MeSH
- nemoci psů * mikrobiologie MeSH
- plazmidy genetika MeSH
- psi MeSH
- sekvenování celého genomu metody MeSH
- zvířata MeSH
- Check Tag
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Antimicrobial consumption in veterinary medicine has led to the spread of multi drug-resistance in clinically important bacteria, with the companion animals and their environment involved as emerging reservoirs. While CTX-M-15 and CMY-2 acquired β-lactamases have been widely detected in the bacterial population of companion and breeding animals in European area, DHA-1 enzymes have been rarely reported in veterinary medicine. The aim of the study was to characterize the Escherichia coli associated with mortality of a litter of Bulldog puppies in a breeding kennel located in Pesaro area, Central Italy. The E. coli strains O39 serotype were resistant to 3rd/4th generation cephalosporins, chloramphenicol, aminoglycosides, trimethoprim-sulfamethoxazole, and ciprofloxacin, retaining susceptibility to carbapenems, colistin, fosfomycin, and levofloxacin (by Microscan Autoscan4, EUCAST clinical breakpoints). Pulse field gel electrophoreses (PFGE-XbaI) on five E. coli strains revealed the presence of a single profile. Whole genome sequencing (WGS) analysis revealed a complex resistome, harboring blaTEM-1b, blaCTX-M-15, blaOXA-1, aph(6)-Ib, aac(6')Ib-cr, aac(3)-Ila, aph(6)-Id, aadA1, qnrB1, sul2, catA1, catB3, tetA, and dfrA14 genes located on a 302597 bp IncHI2/HI2A plasmid. Moreover, blaDHA-1, qnrB4, mph(A), sul1, and dfrA17 determinants were carried on an 83,429 bp IncFII plasmid. A blaCMY-2 determinant was carried on a 90,249 bp IncI1 plasmid. Two IncX1 and IncX4 plasmids without antimicrobial resistance genes were also detected. The presence of lpfA, iss, astA, and gad virulence factors was highlighted. This is the first report in Italy on an invasive infection in eight 2-weeks old dogs caused by the same MDR E. coli O39 blaCTX-M-15, blaCMY-2, blaDHA-1, and aac(6')-Ib-cr positive strain. The above MDR E. coli clone caused the death of the entire litter, despite amoxicillin-clavulanate and enrofloxacin administration. The tank for storage of the water used to prepare the milk-based meal for the litter was the suspected reservoir.
Biomedical Center Faculty of Medicine in Pilsen Charles University Pilsen Czechia
Department of Microbiology Faculty of Medicine in Pilsen Charles University Pilsen Czechia
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