Streptomyces sp. TR1341 was isolated from the sputum of a man with a history of lung and kidney tuberculosis, recurrent respiratory infections, and COPD. It produces secondary metabolites associated with cytotoxicity and immune response modulation. In this study, we complement our previous results by identifying the genetic features associated with the production of these secondary metabolites and other characteristics that could benefit the strain during its colonization of human tissues (virulence factors, modification of the host immune response, or the production of siderophores). We performed a comparative phylogenetic analysis to identify the genetic features that are shared by environmental isolates and human respiratory pathogens. The results showed a high genomic similarity of Streptomyces sp. TR1341 to the plant-associated Streptomyces sp. endophyte_N2, inferring a soil origin of the strain. Putative virulence genes, such as mammalian cell entry (mce) genes were not detected in the TR1341's genome. The presence of a type VII secretion system, distinct from the ones found in Mycobacterium species, suggests a different colonization strategy than the one used by other actinomycete lung pathogens. We identified a higher diversity of genes related to iron acquisition and demonstrated that the strain produces ferrioxamine B in vitro. These results indicate that TR1341 may have an advantage in colonizing environments that are low in iron, such as human tissue.

Faculty of Fisheries and Protection of Waters University of South Bohemia Zátiší 728 2 38925 Vodňany Czech Republic

Faculty of Science University of South Bohemia Branišovská 1645 31a 37005 České Budějovice Czech Republic

Institute of Immunology and Microbiology 1st Faculty of Medicine Charles University Studničkova 7 12800 Prague 2 Czech Republic

Institute of Soil Biology Biology Centre Academy of Sciences of The Czech Republic Na Sádkách 702 7 37005 České Budějovice Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc22008299

003      

CZ-PrNML

005      

20230113093838.0

007      

ta

008      

220317s2021 sz f 000 0|eng||

009      

AR

024    7_

$a 10.3390/microorganisms9081547 $2 doi

035    __

$a (PubMed)34442631

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a sz

100    1_

$a Lara, Ana Catalina $u Institute of Soil Biology, Biology Centre Academy of Sciences of The Czech Republic, Na Sádkách 702/7, 37005 České Budějovice, Czech Republic $1 https://orcid.org/0000000346622596

245    14

$a The Genome Analysis of the Human Lung-Associated Streptomyces sp. TR1341 Revealed the Presence of Beneficial Genes for Opportunistic Colonization of Human Tissues / $c AC. Lara, E. Corretto, L. Kotrbová, F. Lorenc, K. Petříčková, R. Grabic, A. Chroňáková

520    9_

$a Streptomyces sp. TR1341 was isolated from the sputum of a man with a history of lung and kidney tuberculosis, recurrent respiratory infections, and COPD. It produces secondary metabolites associated with cytotoxicity and immune response modulation. In this study, we complement our previous results by identifying the genetic features associated with the production of these secondary metabolites and other characteristics that could benefit the strain during its colonization of human tissues (virulence factors, modification of the host immune response, or the production of siderophores). We performed a comparative phylogenetic analysis to identify the genetic features that are shared by environmental isolates and human respiratory pathogens. The results showed a high genomic similarity of Streptomyces sp. TR1341 to the plant-associated Streptomyces sp. endophyte_N2, inferring a soil origin of the strain. Putative virulence genes, such as mammalian cell entry (mce) genes were not detected in the TR1341's genome. The presence of a type VII secretion system, distinct from the ones found in Mycobacterium species, suggests a different colonization strategy than the one used by other actinomycete lung pathogens. We identified a higher diversity of genes related to iron acquisition and demonstrated that the strain produces ferrioxamine B in vitro. These results indicate that TR1341 may have an advantage in colonizing environments that are low in iron, such as human tissue.

650    17

$a Streptomyces $x genetika $x izolace a purifikace $7 D013302 $2 czmesh

650    _7

$a genom $x genetika $7 D016678 $2 czmesh

650    _7

$a geny $x genetika $7 D005796 $2 czmesh

650    _7

$a tkáně $x mikrobiologie $7 D014024 $2 czmesh

650    _7

$a plíce $x mikrobiologie $7 D008168 $2 czmesh

650    _7

$a počet mikrobiálních kolonií $x metody $7 D015169 $2 czmesh

650    _7

$a fylogeneze $7 D010802 $2 czmesh

650    _7

$a genetické techniky $7 D005821 $2 czmesh

650    _7

$a lidé $7 D006801 $2 czmesh

655    _2

$a časopisecké články $7 D016428

655    _7

$a práce podpořená grantem $7 D013485 $2 czmesh

700    1_

$a Corretto, Erika $u Institute of Soil Biology, Biology Centre Academy of Sciences of The Czech Republic, Na Sádkách 702/7, 37005 České Budějovice, Czech Republic $1 https://orcid.org/0000000277675191

700    1_

$a Kotrbová, Lucie $u Institute of Soil Biology, Biology Centre Academy of Sciences of The Czech Republic, Na Sádkách 702/7, 37005 České Budějovice, Czech Republic

700    1_

$a Lorenc, František $u Institute of Soil Biology, Biology Centre Academy of Sciences of The Czech Republic, Na Sádkách 702/7, 37005 České Budějovice, Czech Republic $1 https://orcid.org/0000000249863160

700    1_

$a Petříčková, Kateřina $u Institute of Immunology and Microbiology, 1st Faculty of Medicine, Charles University, Studničkova 7, 12800 Prague 2, Czech Republic $u Faculty of Science, University of South Bohemia, Branišovská 1645/31a, 37005 České Budějovice, Czech Republic $1 https://orcid.org/000000033719354X $7 xx0104744

700    1_

$a Grabic, Roman $u Faculty of Fisheries and Protection of Waters, University of South Bohemia, Zátiší 728/II, 38925 Vodňany, Czech Republic

700    1_

$a Chroňáková, Alica $u Institute of Soil Biology, Biology Centre Academy of Sciences of The Czech Republic, Na Sádkách 702/7, 37005 České Budějovice, Czech Republic $1 https://orcid.org/0000000233166862 $7 osd2016936705

773    0_

$w MED00198767 $t Microorganisms $x 2076-2607 $g Roč. 9, č. 8 (2021)

856    41

$u https://pubmed.ncbi.nlm.nih.gov/34442631 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y p $z 0

990    __

$a 20220317 $b ABA008

991    __

$a 20230113093830 $b ABA008

999    __

$a kom $b bmc $g 1771004 $s 1159493

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2021 $b 9 $c 8 $e 20210721 $i 2076-2607 $m Microorganisms $n Microorganisms $x MED00198767

GRA    __

$a 17-30091A $p Ministerstvo Zdravotnictví Ceské Republiky

GRA    __

$a LM2018129 $p Ministerstvo Školství, Mládeže a Tělovýchovy

GRA    __

$a No. CZ.02.1.01/0.0/0.0/16_013/0001775 $p European Regional Development Fund

GRA    __

$a NV17-30091A $p MZ0

LZP    __

$c NLK120 $d 20230113 $a 2021-granty