Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Mitochondrial respiration of human platelets in young adult and advanced age - Seahorse or O2k

J. Jedlička, R. Kunc, J. Kuncová

. 2021 ; 70 (Suppl. 3) : S369-S379. [pub] 20211231

Jazyk angličtina Země Česko

Typ dokumentu srovnávací studie, časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22009468

The objective of the present study was to evaluate platelet mitochondrial oxygen consumption using high-resolution respirometry (HRR) and metabolic flux analysis (MFA) and to verify the effect of advanced age on these parameters. HRR was used to analyze permeabilized and intact platelets, MFA to measure oxygen consumption rates (OCR), extracellular acidification rates (ECAR) and ATP production rate in intact fixed platelets. Two groups of healthy volunteers were included in the study: YOUNG (20-42 years, n=44) and older adults (OLD; 70-89 years; n=15). Compared to YOUNG donors, platelets from group OLD participants displayed significantly lower values of oxygen consumption in the Complex II-linked phosphorylating and uncoupled states and the Complex IV activity in HRR protocols for permeabilized cells and significantly lower resting and uncoupled respirations in intact cells when analyzed by both methods. In addition, mitochondrial ATP production rate was also significantly lower in platelets isolated from older adults. Variables measured by both methods from the same bloods correlated significantly, nevertheless those acquired by MFA were higher than those measured using HRR. In conclusion, the study verifies compromised mitochondrial respiration and oxidative ATP production in the platelets of aged persons and documents good compatibility of the two most widely used methods for determining the global performance of the electron-transporting system, i.e. HRR and MFA.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22009468
003      
CZ-PrNML
005      
20250625104159.0
007      
ta
008      
220419s2021 xr d f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.934812 $2 doi
035    __
$a (PubMed)35099255
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Jedlička, Jan, $d 1991- $u Institute of Physiology, Faculty of Medicine in Plzeň, Charles University, Plzeň, Czech Republic $7 xx0230803 $u Biomedical Centre, Faculty of Medicine in Plzeň, Charles University, Plzeň, Czech Republic
245    10
$a Mitochondrial respiration of human platelets in young adult and advanced age - Seahorse or O2k / $c J. Jedlička, R. Kunc, J. Kuncová
520    9_
$a The objective of the present study was to evaluate platelet mitochondrial oxygen consumption using high-resolution respirometry (HRR) and metabolic flux analysis (MFA) and to verify the effect of advanced age on these parameters. HRR was used to analyze permeabilized and intact platelets, MFA to measure oxygen consumption rates (OCR), extracellular acidification rates (ECAR) and ATP production rate in intact fixed platelets. Two groups of healthy volunteers were included in the study: YOUNG (20-42 years, n=44) and older adults (OLD; 70-89 years; n=15). Compared to YOUNG donors, platelets from group OLD participants displayed significantly lower values of oxygen consumption in the Complex II-linked phosphorylating and uncoupled states and the Complex IV activity in HRR protocols for permeabilized cells and significantly lower resting and uncoupled respirations in intact cells when analyzed by both methods. In addition, mitochondrial ATP production rate was also significantly lower in platelets isolated from older adults. Variables measured by both methods from the same bloods correlated significantly, nevertheless those acquired by MFA were higher than those measured using HRR. In conclusion, the study verifies compromised mitochondrial respiration and oxidative ATP production in the platelets of aged persons and documents good compatibility of the two most widely used methods for determining the global performance of the electron-transporting system, i.e. HRR and MFA.
650    _2
$a adenosintrifosfát $x metabolismus $7 D000255
650    _2
$a dospělí $7 D000328
650    _2
$a věkové faktory $7 D000367
650    _2
$a senioři $7 D000368
650    _2
$a senioři nad 80 let $7 D000369
650    _2
$a stárnutí $x krev $x metabolismus $7 D000375
650    _2
$a trombocyty $x metabolismus $7 D001792
650    _2
$a buněčné dýchání $7 D019069
650    12
$a energetický metabolismus $7 D004734
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a koncentrace vodíkových iontů $7 D006863
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a analýza metabolického toku $x metody $7 D064688
650    _2
$a mitochondrie $x metabolismus $7 D008928
650    _2
$a spotřeba kyslíku $7 D010101
650    _2
$a mladý dospělý $7 D055815
655    _2
$a srovnávací studie $7 D003160
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kunc, Radovan $7 xx0301066 $u Institute of Social Medicine, Faculty of Medicine in Plzeň, Charles University, Plzeň, Czech Republic $u Hospice of St. Lazar, Plzeň, Czech Republic
700    1_
$a Kuncová, Jitka, $d 1964- $7 xx0078884 $u Institute of Physiology, Faculty of Medicine in Plzeň, Charles University, Plzeň, Czech Republic $u Biomedical Centre, Faculty of Medicine in Plzeň, Charles University, Plzeň, Czech Republic
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 70, Suppl. 3 (2021), s. S369-S379
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35099255 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y - $z 0
990    __
$a 20220419 $b ABA008
991    __
$a 20250625104154 $b ABA008
999    __
$a kom $b bmc $g 1796976 $s 1160666
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 70 $c Suppl. 3 $d S369-S379 $e 20211231 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK124 $a Pubmed-20220419

Najít záznam

Citační ukazatele

Možnosti archivace