• Something wrong with this record ?

MyomiRs in cultured muscle cells from patients with idiopathic inflammatory myopathy are modulated by disease but not by 6-month exercise training

N. Alchus Laiferová, M. Nemec, L. Vernerová, M. Balážová, M. Vokurková, S. Oreská, M. Klein, M. Špiritović, M. Tomčík, J. Vencovský, J. Ukropec, B. Ukropcová

. 2022 ; 40 (2) : 346-357. [pub] 20220225

Language English Country Italy

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22011111

OBJECTIVES: Idiopathic inflammatory myopathies/IIM are associated with changes in muscle-specific microRNA/miR. Exercise improves muscle function and metabolism in parallel with changes in miR expression. We investigated the effects of disease and exercise on miRs in differentiated muscle cells/myotubes from IIM patients and controls. METHODS: Samples of m. vastus lateralis were obtained by needle biopsy from IIM patients before/after 6-month training and from matched sedentary healthy controls. Muscle cell cultures were established and exposed to saturated fatty acid during differentiation. MiR-133a,-133b,-206,-1 and their target genes (qPCR), fat oxidation (FOx), lipids (chromatography) and mitochondrial oxidative phosphorylation (OxPHOS) complexes (immunoblotting) were measured. Interrelations between in vitro miRs and metabolism of myotubes as well as clinical parameters and disease activity/MITAX were explored. RESULTS: Levels of miRs were higher in myotubes derived from IIM patients compared to healthy controls (up to 3.5-fold, p<0.05). Neither 6-month training (IIM patients) nor in vitro palmitate treatment modulated myomiRs in myotubes. However, miR-133a,-133b, and miR-1 correlated negatively with FOx (p<0.01), triacylglycerols (p<0.05) and OxPHOS complex-V (p<0.05) and positively with OxPHOS complex-I (p<0.05) in myotubes. MiR-133a and miR-133b in myotubes were related to disease activity and fasting glycaemia in vivo (both p<0.05). CONCLUSIONS: Upregulation of microRNAs involved in myogenesis and regeneration in muscle cells derived from IIM patients indicates activation of compensatory epigenetic mechanisms, potentially aimed to counteract disease progression. Relationships of microRNAs with in vitro metabolic profile of muscle cells as well as with clinical parameters support the role of muscle-specific microRNAs in modulating muscle metabolism and clinical state of patients.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22011111
003      
CZ-PrNML
005      
20220506125834.0
007      
ta
008      
220425s2022 it f 000 0|eng||
009      
AR
024    7_
$a 10.55563/clinexprheumatol/69z7za $2 doi
035    __
$a (PubMed)35225219
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a it
100    1_
$a Alchus Laiferová, Nikoleta $u Institute of Experimental Endocrinology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava, and Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia
245    10
$a MyomiRs in cultured muscle cells from patients with idiopathic inflammatory myopathy are modulated by disease but not by 6-month exercise training / $c N. Alchus Laiferová, M. Nemec, L. Vernerová, M. Balážová, M. Vokurková, S. Oreská, M. Klein, M. Špiritović, M. Tomčík, J. Vencovský, J. Ukropec, B. Ukropcová
520    9_
$a OBJECTIVES: Idiopathic inflammatory myopathies/IIM are associated with changes in muscle-specific microRNA/miR. Exercise improves muscle function and metabolism in parallel with changes in miR expression. We investigated the effects of disease and exercise on miRs in differentiated muscle cells/myotubes from IIM patients and controls. METHODS: Samples of m. vastus lateralis were obtained by needle biopsy from IIM patients before/after 6-month training and from matched sedentary healthy controls. Muscle cell cultures were established and exposed to saturated fatty acid during differentiation. MiR-133a,-133b,-206,-1 and their target genes (qPCR), fat oxidation (FOx), lipids (chromatography) and mitochondrial oxidative phosphorylation (OxPHOS) complexes (immunoblotting) were measured. Interrelations between in vitro miRs and metabolism of myotubes as well as clinical parameters and disease activity/MITAX were explored. RESULTS: Levels of miRs were higher in myotubes derived from IIM patients compared to healthy controls (up to 3.5-fold, p<0.05). Neither 6-month training (IIM patients) nor in vitro palmitate treatment modulated myomiRs in myotubes. However, miR-133a,-133b, and miR-1 correlated negatively with FOx (p<0.01), triacylglycerols (p<0.05) and OxPHOS complex-V (p<0.05) and positively with OxPHOS complex-I (p<0.05) in myotubes. MiR-133a and miR-133b in myotubes were related to disease activity and fasting glycaemia in vivo (both p<0.05). CONCLUSIONS: Upregulation of microRNAs involved in myogenesis and regeneration in muscle cells derived from IIM patients indicates activation of compensatory epigenetic mechanisms, potentially aimed to counteract disease progression. Relationships of microRNAs with in vitro metabolic profile of muscle cells as well as with clinical parameters support the role of muscle-specific microRNAs in modulating muscle metabolism and clinical state of patients.
650    _2
$a kultivované buňky $7 D002478
650    _2
$a cvičení $x fyziologie $7 D015444
650    _2
$a lidé $7 D006801
650    12
$a mikro RNA $x genetika $x metabolismus $7 D035683
650    _2
$a kosterní svalová vlákna $x metabolismus $x patologie $7 D018485
650    _2
$a kosterní svaly $x fyziologie $7 D018482
650    12
$a myozitida $x patologie $7 D009220
655    _2
$a časopisecké články $7 D016428
700    1_
$a Nemec, Michal $u Institute of Experimental Endocrinology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava, Slovakia
700    1_
$a Vernerová, Lucia $u Institute of Rheumatology, Prague, Czech Republic
700    1_
$a Balážová, Mária $u Centre of Biosciences, Slovak Academy of Sciences, Bratislava, Slovakia
700    1_
$a Vokurková, Martina $u Institute of Rheumatology, Prague, Czech Republic
700    1_
$a Oreská, Sabína $u Institute of Rheumatology, Prague, and Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Klein, Martin $u Institute of Rheumatology, Prague, and Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Špiritović, Maja $u Institute of Rheumatology, Prague, and Department of Physiotherapy, Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic
700    1_
$a Tomčík, Michal $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Vencovský, Jiří $u Institute of Rheumatology, Prague, and Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Ukropec, Jozef $u Institute of Experimental Endocrinology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava, Slovakia
700    1_
$a Ukropcová, Barbara $u Institute of Experimental Endocrinology, Biomedical Research Centre, Slovak Academy of Sciences, Bratislava, and Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia. barbara.ukropcova@savba.sk
773    0_
$w MED00001116 $t Clinical and experimental rheumatology $x 0392-856X $g Roč. 40, č. 2 (2022), s. 346-357
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35225219 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20220506125826 $b ABA008
999    __
$a ok $b bmc $g 1788947 $s 1162309
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 40 $c 2 $d 346-357 $e 20220225 $i 0392-856X $m Clinical and Experimental Rheumatology $n Clin Exp Rheumatol $x MED00001116
LZP    __
$a Pubmed-20220425

Find record

Citation metrics

Archiving options