• Je něco špatně v tomto záznamu ?

Effect of Atmospheric Aging on Soot Particle Toxicity in Lung Cell Models at the Air-Liquid Interface: Differential Toxicological Impacts of Biogenic and Anthropogenic Secondary Organic Aerosols (SOAs)

S. Offer, E. Hartner, S. Di Bucchianico, C. Bisig, S. Bauer, J. Pantzke, EJ. Zimmermann, X. Cao, S. Binder, E. Kuhn, A. Huber, S. Jeong, U. Käfer, P. Martens, A. Mesceriakovas, J. Bendl, R. Brejcha, A. Buchholz, D. Gat, T. Hohaus, N. Rastak, G....

. 2022 ; 130 (2) : 27003. [pub] 20220203

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22011125

BACKGROUND: Secondary organic aerosols (SOAs) formed from anthropogenic or biogenic gaseous precursors in the atmosphere substantially contribute to the ambient fine particulate matter [PM ≤2.5μm in aerodynamic diameter (PM2.5)] burden, which has been associated with adverse human health effects. However, there is only limited evidence on their differential toxicological impact. OBJECTIVES: We aimed to discriminate toxicological effects of aerosols generated by atmospheric aging on combustion soot particles (SPs) of gaseous biogenic (β-pinene) or anthropogenic (naphthalene) precursors in two different lung cell models exposed at the air-liquid interface (ALI). METHODS: Mono- or cocultures of lung epithelial cells (A549) and endothelial cells (EA.hy926) were exposed at the ALI for 4 h to different aerosol concentrations of a photochemically aged mixture of primary combustion SP and β-pinene (SOAβPIN-SP) or naphthalene (SOANAP-SP). The internally mixed soot/SOA particles were comprehensively characterized in terms of their physical and chemical properties. We conducted toxicity tests to determine cytotoxicity, intracellular oxidative stress, primary and secondary genotoxicity, as well as inflammatory and angiogenic effects. RESULTS: We observed considerable toxicity-related outcomes in cells treated with either SOA type. Greater adverse effects were measured for SOANAP-SP compared with SOAβPIN-SP in both cell models, whereas the nano-sized soot cores alone showed only minor effects. At the functional level, we found that SOANAP-SP augmented the secretion of malondialdehyde and interleukin-8 and may have induced the activation of endothelial cells in the coculture system. This activation was confirmed by comet assay, suggesting secondary genotoxicity and greater angiogenic potential. Chemical characterization of PM revealed distinct qualitative differences in the composition of the two secondary aerosol types. DISCUSSION: In this study using A549 and EA.hy926 cells exposed at ALI, SOA compounds had greater toxicity than primary SPs. Photochemical aging of naphthalene was associated with the formation of more oxidized, more aromatic SOAs with a higher oxidative potential and toxicity compared with β-pinene. Thus, we conclude that the influence of atmospheric chemistry on the chemical PM composition plays a crucial role for the adverse health outcome of emissions. https://doi.org/10.1289/EHP9413.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22011125
003      
CZ-PrNML
005      
20220506125851.0
007      
ta
008      
220425s2022 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1289/EHP9413 $2 doi
035    __
$a (PubMed)35112925
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Offer, Svenja $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany
245    10
$a Effect of Atmospheric Aging on Soot Particle Toxicity in Lung Cell Models at the Air-Liquid Interface: Differential Toxicological Impacts of Biogenic and Anthropogenic Secondary Organic Aerosols (SOAs) / $c S. Offer, E. Hartner, S. Di Bucchianico, C. Bisig, S. Bauer, J. Pantzke, EJ. Zimmermann, X. Cao, S. Binder, E. Kuhn, A. Huber, S. Jeong, U. Käfer, P. Martens, A. Mesceriakovas, J. Bendl, R. Brejcha, A. Buchholz, D. Gat, T. Hohaus, N. Rastak, G. Jakobi, M. Kalberer, T. Kanashova, Y. Hu, C. Ogris, A. Marsico, F. Theis, M. Pardo, T. Gröger, S. Oeder, J. Orasche, A. Paul, T. Ziehm, ZH. Zhang, T. Adam, O. Sippula, M. Sklorz, J. Schnelle-Kreis, H. Czech, A. Kiendler-Scharr, Y. Rudich, R. Zimmermann
520    9_
$a BACKGROUND: Secondary organic aerosols (SOAs) formed from anthropogenic or biogenic gaseous precursors in the atmosphere substantially contribute to the ambient fine particulate matter [PM ≤2.5μm in aerodynamic diameter (PM2.5)] burden, which has been associated with adverse human health effects. However, there is only limited evidence on their differential toxicological impact. OBJECTIVES: We aimed to discriminate toxicological effects of aerosols generated by atmospheric aging on combustion soot particles (SPs) of gaseous biogenic (β-pinene) or anthropogenic (naphthalene) precursors in two different lung cell models exposed at the air-liquid interface (ALI). METHODS: Mono- or cocultures of lung epithelial cells (A549) and endothelial cells (EA.hy926) were exposed at the ALI for 4 h to different aerosol concentrations of a photochemically aged mixture of primary combustion SP and β-pinene (SOAβPIN-SP) or naphthalene (SOANAP-SP). The internally mixed soot/SOA particles were comprehensively characterized in terms of their physical and chemical properties. We conducted toxicity tests to determine cytotoxicity, intracellular oxidative stress, primary and secondary genotoxicity, as well as inflammatory and angiogenic effects. RESULTS: We observed considerable toxicity-related outcomes in cells treated with either SOA type. Greater adverse effects were measured for SOANAP-SP compared with SOAβPIN-SP in both cell models, whereas the nano-sized soot cores alone showed only minor effects. At the functional level, we found that SOANAP-SP augmented the secretion of malondialdehyde and interleukin-8 and may have induced the activation of endothelial cells in the coculture system. This activation was confirmed by comet assay, suggesting secondary genotoxicity and greater angiogenic potential. Chemical characterization of PM revealed distinct qualitative differences in the composition of the two secondary aerosol types. DISCUSSION: In this study using A549 and EA.hy926 cells exposed at ALI, SOA compounds had greater toxicity than primary SPs. Photochemical aging of naphthalene was associated with the formation of more oxidized, more aromatic SOAs with a higher oxidative potential and toxicity compared with β-pinene. Thus, we conclude that the influence of atmospheric chemistry on the chemical PM composition plays a crucial role for the adverse health outcome of emissions. https://doi.org/10.1289/EHP9413.
650    _2
$a aerosoly $x analýza $7 D000336
650    _2
$a senioři $7 D000368
650    _2
$a stárnutí $7 D000375
650    12
$a látky znečišťující vzduch $x analýza $x toxicita $7 D000393
650    _2
$a endoteliální buňky $x chemie $x metabolismus $7 D042783
650    _2
$a lidé $7 D006801
650    _2
$a plíce $x metabolismus $7 D008168
650    _2
$a pevné částice $x analýza $7 D052638
650    12
$a saze $7 D053260
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Hartner, Elena $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany
700    1_
$a Di Bucchianico, Sebastiano $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $1 https://orcid.org/000000026396892X
700    1_
$a Bisig, Christoph $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $1 https://orcid.org/0000000199811409
700    1_
$a Bauer, Stefanie $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Pantzke, Jana $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany $1 https://orcid.org/0000000154208905
700    1_
$a Zimmermann, Elias J $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany $1 https://orcid.org/000000028766180X
700    1_
$a Cao, Xin $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany
700    1_
$a Binder, Stefanie $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany
700    1_
$a Kuhn, Evelyn $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Huber, Anja $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Jeong, Seongho $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany $1 https://orcid.org/0000000180577827
700    1_
$a Käfer, Uwe $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany $1 https://orcid.org/0000000240160661
700    1_
$a Martens, Patrick $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany $1 https://orcid.org/000000025719872X
700    1_
$a Mesceriakovas, Arunas $u Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio, Finland $1 https://orcid.org/0000000232575293
700    1_
$a Bendl, Jan $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u Institute for Chemistry and Environmental Engineering, University of the Bundeswehr Munich, Neubiberg, Germany $u Institute for Environmental Studies, Faculty of Science, Charles University, Prague, Czech Republic $1 https://orcid.org/0000000323225483
700    1_
$a Brejcha, Ramona $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Buchholz, Angela $u Department of Applied Physics, University of Eastern Finland, Kuopio, Finland $1 https://orcid.org/0000000271191452
700    1_
$a Gat, Daniella $u Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, Rehovot, Israel $1 https://orcid.org/0000000205954626
700    1_
$a Hohaus, Thorsten $u Institute of Energy and Climate Research, Troposphere, Forschungszentrum Jülich GmbH, Jülich, Germany $1 https://orcid.org/0000000157226244
700    1_
$a Rastak, Narges $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Jakobi, Gert $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Kalberer, Markus $u Department of Environmental Sciences, University of Basel, Basel, Switzerland $1 https://orcid.org/0000000188856556
700    1_
$a Kanashova, Tamara $u Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany
700    1_
$a Hu, Yue $u Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany $1 https://orcid.org/0000000338113206
700    1_
$a Ogris, Christoph $u Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Marsico, Annalisa $u Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Theis, Fabian $u Institute of Computational Biology, Helmholtz Zentrum München, Neuherberg, Germany $1 https://orcid.org/0000000224191943
700    1_
$a Pardo, Michal $u Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, Rehovot, Israel $1 https://orcid.org/0000000164801171
700    1_
$a Gröger, Thomas $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Oeder, Sebastian $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Orasche, Jürgen $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Paul, Andreas $u Institute of Energy and Climate Research, Troposphere, Forschungszentrum Jülich GmbH, Jülich, Germany
700    1_
$a Ziehm, Till $u Institute of Energy and Climate Research, Troposphere, Forschungszentrum Jülich GmbH, Jülich, Germany $1 https://orcid.org/0000000155863709
700    1_
$a Zhang, Zhi-Hui $u Department of Environmental Sciences, University of Basel, Basel, Switzerland
700    1_
$a Adam, Thomas $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u Institute for Chemistry and Environmental Engineering, University of the Bundeswehr Munich, Neubiberg, Germany
700    1_
$a Sippula, Olli $u Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio, Finland $1 https://orcid.org/0000000269812694
700    1_
$a Sklorz, Martin $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Schnelle-Kreis, Jürgen $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany
700    1_
$a Czech, Hendryk $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany $1 https://orcid.org/0000000183774252
700    1_
$a Kiendler-Scharr, Astrid $u Institute of Energy and Climate Research, Troposphere, Forschungszentrum Jülich GmbH, Jülich, Germany $1 https://orcid.org/0000000331662253
700    1_
$a Rudich, Yinon $u Department of Earth and Planetary Sciences, Faculty of Chemistry, Weizmann Institute of Science, Rehovot, Israel $1 https://orcid.org/0000000331490201
700    1_
$a Zimmermann, Ralf $u Joint Mass Spectrometry Center (JMSC) at Comprehensive Molecular Analytics, Helmholtz Zentrum München, Neuherberg, Germany $u JMSC at Analytical Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany
773    0_
$w MED00001549 $t Environmental health perspectives $x 1552-9924 $g Roč. 130, č. 2 (2022), s. 27003
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35112925 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20220506125843 $b ABA008
999    __
$a ok $b bmc $g 1788956 $s 1162323
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 130 $c 2 $d 27003 $e 20220203 $i 1552-9924 $m Environmental health perspectives $n Environ Health Perspect $x MED00001549
LZP    __
$a Pubmed-20220425

Najít záznam

Citační ukazatele

Možnosti archivace