The aim of this study was to reveal the effects of foodborne fluoxetine on morphological and condition profile, hematological profile, biochemical and oxidative stress indices on juvenile rainbow trout. The study was performed according to OECD Guideline No. 215. Fluoxetine was incorporated into Biomar 921 pellets at a dose of 0.047 mg/kg (environmental concentration), 0.577 mg/kg and 6.7 mg/kg. There was statistically significant change in hematological profile, including an increasing trend in neutrophil/lymphocyte ratio and a decreasing trend in the number of lymphocytes. Measurements of oxidative stress indicated decreased activity of the detoxifying enzyme glutathione-S-transferase in the liver and kidney. However, the measurement of GR, GPx, CAT, SOD activity, and TBARS showed no changes. Histopathological examination revealed damage to the proximal tubules of caudal kidney in exposed groups. This study confirms that fluoxetine has a significant effect on immune response.

Department of Anatomy Histology and Embryology Faculty of Veterinary Medicine University of Veterinary Sciences Brno Czech Republic

Department of Animal Protection and Welfare and Veterinary Public Health Faculty of Veterinary Hygiene and Ecology University of Veterinary Sciences Brno Czech Republic

Department of Pathology Znojmo Hospital Czech Republic

Department of Pharmaceutical Technology Faculty of Pharmacy Masaryk University in Brno Czech Republic

Department of Zoology Fisheries Hydrobiology and Apiculture Faculty of Agrisciences Mendel University in Brno Czech Republic

Veterinary Research Institute Department of Infectious Diseases and Preventive Medicine Brno Czech Republic

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$a Vaclavik, Josef $u Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic

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$a Foodborne fluoxetine impacts the immune response in rainbow trout (Oncorhynchus mykkis) / $c J. Vaclavik, P. Sehonova, J. Blahova, D. Medkova, E. Postulkova, O. Maly, M. Charvatova, K. Stastny, J. Lenz, J. Mares, A. Franc, Z. Svobodova

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$a The aim of this study was to reveal the effects of foodborne fluoxetine on morphological and condition profile, hematological profile, biochemical and oxidative stress indices on juvenile rainbow trout. The study was performed according to OECD Guideline No. 215. Fluoxetine was incorporated into Biomar 921 pellets at a dose of 0.047 mg/kg (environmental concentration), 0.577 mg/kg and 6.7 mg/kg. There was statistically significant change in hematological profile, including an increasing trend in neutrophil/lymphocyte ratio and a decreasing trend in the number of lymphocytes. Measurements of oxidative stress indicated decreased activity of the detoxifying enzyme glutathione-S-transferase in the liver and kidney. However, the measurement of GR, GPx, CAT, SOD activity, and TBARS showed no changes. Histopathological examination revealed damage to the proximal tubules of caudal kidney in exposed groups. This study confirms that fluoxetine has a significant effect on immune response.

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$a Lakdawala, Pavla $u Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic. Electronic address: sehonovap@vfu.cz $7 xx0267005

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$a Blahova, Jana $u Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic

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$a Medkova, Denisa $u Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic; Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agrisciences, Mendel University in Brno, Czech Republic

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$a Postulkova, Eva $u Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agrisciences, Mendel University in Brno, Czech Republic

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$a Maly, Ondrej $u Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agrisciences, Mendel University in Brno, Czech Republic

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$a Charvatova, Michaela $u Veterinary Research Institute, Department of Infectious Diseases and Preventive Medicine, Brno, Czech Republic

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$a Stastny, Kamil $u Veterinary Research Institute, Department of Infectious Diseases and Preventive Medicine, Brno, Czech Republic

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$a Lenz, Jiri $u Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary Sciences, Brno, Czech Republic; Department of Pathology, Znojmo Hospital, Czech Republic

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$a Mares, Jan $u Department of Zoology, Fisheries, Hydrobiology and Apiculture, Faculty of Agrisciences, Mendel University in Brno, Czech Republic

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$a Franc, Ales $u Department of Pharmaceutical Technology, Faculty of Pharmacy, Masaryk University in Brno, Czech Republic

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$a Svobodova, Zdenka $u Department of Animal Protection and Welfare & Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech Republic

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