• Je něco špatně v tomto záznamu ?

Antiproliferative and Cytotoxic Activities of Fluorescein-A Diagnostic Angiography Dye

M. Šranková, A. Dvořák, M. Martínek, P. Šebej, P. Klán, L. Vítek, L. Muchová

. 2022 ; 23 (3) : . [pub] 20220128

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22011245

Grantová podpora
MH-DRO-VFN64165 Ministry of Health of the Czech Republic
GJ20-30004Y Czech Science Foundation
GAUK 314621 Charles University

Fluorescein is a fluorescent dye used as a diagnostic tool in various fields of medicine. Although fluorescein itself possesses low toxicity, after photoactivation, it releases potentially toxic molecules, such as singlet oxygen (1O2) and, as we demonstrate in this work, also carbon monoxide (CO). As both of these molecules can affect physiological processes, the main aim of this study was to explore the potential biological impacts of fluorescein photochemistry. In our in vitro study in a human hepatoblastoma HepG2 cell line, we explored the possible effects on cell viability, cellular energy metabolism, and the cell cycle. We observed markedly lowered cell viability (≈30%, 75-2400 μM) upon irradiation of intracellular fluorescein and proved that this decrease in viability was dependent on the cellular oxygen concentration. We also detected a significantly decreased concentration of Krebs cycle metabolites (lactate and citrate < 30%; 2-hydroxyglutarate and 2-oxoglutarate < 10%) as well as cell cycle arrest (decrease in the G2 phase of 18%). These observations suggest that this photochemical reaction could have important biological consequences and may account for some adverse reactions observed in fluorescein-treated patients. Additionally, the biological activities of both 1O2 and CO might have considerable therapeutic potential, particularly in the treatment of cancer.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22011245
003      
CZ-PrNML
005      
20220506130937.0
007      
ta
008      
220425s2022 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ijms23031504 $2 doi
035    __
$a (PubMed)35163426
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Šranková, Mária $u Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital in Prague and 1st Faculty of Medicine, Charles University, Na Bojišti 3, 121 08 Praha, Czech Republic
245    10
$a Antiproliferative and Cytotoxic Activities of Fluorescein-A Diagnostic Angiography Dye / $c M. Šranková, A. Dvořák, M. Martínek, P. Šebej, P. Klán, L. Vítek, L. Muchová
520    9_
$a Fluorescein is a fluorescent dye used as a diagnostic tool in various fields of medicine. Although fluorescein itself possesses low toxicity, after photoactivation, it releases potentially toxic molecules, such as singlet oxygen (1O2) and, as we demonstrate in this work, also carbon monoxide (CO). As both of these molecules can affect physiological processes, the main aim of this study w $a Fluorescein is a fluorescent dye used as a diagnostic tool in various fields of medicine Although fluorescein itself possesses low toxicity after photoactivation it releases potentially toxic molecules such as singlet oxygen 1O2 and as we demonstrate in this work also carbon monoxide CO As both of these molecules can affect physiological processes the main aim of this study was to explor $a Fluorescein is a fluorescent dye used as a diagnostic tool in various fields of medicine. Although fluorescein itself possesses low toxicity, after photoactivation, it releases potentially toxic molecules, such as singlet oxygen (1O2) and, as we demonstrate in this work, also carbon monoxide (CO). As both of these molecules can affect physiological processes, the main aim of this study was to explore the potential biological impacts of fluorescein photochemistry. In our in vitro study in a human hepatoblastoma HepG2 cell line, we explored the possible effects on cell viability, cellular energy metabolism, and the cell cycle. We observed markedly lowered cell viability (≈30%, 75-2400 μM) upon irradiation of intracellular fluorescein and proved that this decrease in viability was dependent on the cellular oxygen concentration. We also detected a significantly decreased concentration of Krebs cycle metabolites (lactate and citrate < 30%; 2-hydroxyglutarate and 2-oxoglutarate < 10%) as well as cell cycle arrest (decrease in the G2 phase of 18%). These observations suggest that this photochemical reaction could have important biological consequences and may account for some adverse reactions observed in fluorescein-treated patients. Additionally, the biological activities of both 1O2 and CO might have considerable therapeutic potential, particularly in the treatment of cancer.
650    _2
$a angiografie $7 D000792
650    _2
$a protinádorové látky $x chemie $x farmakologie $7 D000970
650    _2
$a oxid uhelnatý $x analýza $7 D002248
650    _2
$a kontrolní body buněčného cyklu $x účinky léků $x účinky záření $7 D059447
650    _2
$a viabilita buněk $x účinky léků $7 D002470
650    _2
$a citrátový cyklus $x účinky léků $x účinky záření $7 D002952
650    _2
$a fluorescein $x chemie $x farmakologie $7 D019793
650    _2
$a plynová chromatografie s hmotnostně spektrometrickou detekcí $7 D008401
650    _2
$a buňky Hep G2 $7 D056945
650    _2
$a lidé $7 D006801
650    _2
$a světlo $7 D008027
650    _2
$a fotochemické procesy $7 D055668
650    _2
$a singletový kyslík $x analýza $7 D026082
655    _2
$a časopisecké články $7 D016428
700    1_
$a Dvořák, Aleš $u Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital in Prague and 1st Faculty of Medicine, Charles University, Na Bojišti 3, 121 08 Praha, Czech Republic $1 https://orcid.org/0000000212856928
700    1_
$a Martínek, Marek $u RECETOX, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
700    1_
$a Šebej, Peter $u RECETOX, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
700    1_
$a Klán, Petr $u RECETOX, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic $u Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic $1 https://orcid.org/0000000162872742 $7 ola2003204936
700    1_
$a Vítek, Libor $u Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital in Prague and 1st Faculty of Medicine, Charles University, Na Bojišti 3, 121 08 Praha, Czech Republic $u 4th Department of Internal Medicine, General University Hospital in Prague and 1st Faculty of Medicine, Charles University, U Nemocnice 2, 128 08 Praha, Czech Republic $1 https://orcid.org/0000000253180151 $7 xx0035071
700    1_
$a Muchová, Lucie $u Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital in Prague and 1st Faculty of Medicine, Charles University, Na Bojišti 3, 121 08 Praha, Czech Republic $1 https://orcid.org/0000000320821060 $7 xx0061466
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 23, č. 3 (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35163426 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20220506130929 $b ABA008
999    __
$a ok $b bmc $g 1789044 $s 1162443
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 23 $c 3 $e 20220128 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
GRA    __
$a MH-DRO-VFN64165 $p Ministry of Health of the Czech Republic
GRA    __
$a GJ20-30004Y $p Czech Science Foundation
GRA    __
$a GAUK 314621 $p Charles University
LZP    __
$a Pubmed-20220425

Najít záznam

Citační ukazatele

Možnosti archivace