Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension

S. Dahale, J. Ruiz-Orera, J. Silhavy, N. Hübner, S. van Heesch, M. Pravenec, SS. Atanur

. 2022 ; 5 (4) : . [pub] 20220107

Language English Country United States

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22011372

The role of alternative promoter usage in tissue-specific gene expression has been well established; however, its role in complex diseases is poorly understood. We performed cap analysis of gene expression (CAGE) sequencing from the left ventricle of a rat model of hypertension, the spontaneously hypertensive rat (SHR), and a normotensive strain, Brown Norway to understand the role of alternative promoter usage in complex disease. We identified 26,560 CAGE-defined transcription start sites in the rat left ventricle, including 1,970 novel cardiac transcription start sites. We identified 28 genes with alternative promoter usage between SHR and Brown Norway, which could lead to protein isoforms differing at the amino terminus between two strains and 475 promoter switching events altering the length of the 5' UTR. We found that the shift in Insr promoter usage was significantly associated with insulin levels and blood pressure within a panel of HXB/BXH recombinant inbred rat strains, suggesting that hyperinsulinemia due to insulin resistance might lead to hypertension in SHR. Our study provides a preliminary evidence of alternative promoter usage in complex diseases.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22011372
003      
CZ-PrNML
005      
20220506131457.0
007      
ta
008      
220425s2022 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.26508/lsa.202101234 $2 doi
035    __
$a (PubMed)34996843
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Dahale, Sonal $u Department of Metabolism, Digestion, and Reproduction, Faculty of Medicine, Imperial College London, London, UK $u Department of Microbial Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK
245    10
$a Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension / $c S. Dahale, J. Ruiz-Orera, J. Silhavy, N. Hübner, S. van Heesch, M. Pravenec, SS. Atanur
520    9_
$a The role of alternative promoter usage in tissue-specific gene expression has been well established; however, its role in complex diseases is poorly understood. We performed cap analysis of gene expression (CAGE) sequencing from the left ventricle of a rat model of hypertension, the spontaneously hypertensive rat (SHR), and a normotensive strain, Brown Norway to understand the role of alternative promoter usage in complex disease. We identified 26,560 CAGE-defined transcription start sites in the rat left ventricle, including 1,970 novel cardiac transcription start sites. We identified 28 genes with alternative promoter usage between SHR and Brown Norway, which could lead to protein isoforms differing at the amino terminus between two strains and 475 promoter switching events altering the length of the 5' UTR. We found that the shift in Insr promoter usage was significantly associated with insulin levels and blood pressure within a panel of HXB/BXH recombinant inbred rat strains, suggesting that hyperinsulinemia due to insulin resistance might lead to hypertension in SHR. Our study provides a preliminary evidence of alternative promoter usage in complex diseases.
650    _2
$a zvířata $7 D000818
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a stanovení celkové genové exprese $x metody $7 D020869
650    12
$a hypertenze $x genetika $x metabolismus $7 D006973
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a promotorové oblasti (genetika) $x genetika $7 D011401
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani inbrední SHR $7 D011918
650    _2
$a sekvenční analýza RNA $x metody $7 D017423
650    _2
$a genetická transkripce $x genetika $7 D014158
655    _2
$a časopisecké články $7 D016428
700    1_
$a Ruiz-Orera, Jorge $u Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany
700    1_
$a Silhavy, Jan $u Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Hübner, Norbert $u Cardiovascular and Metabolic Sciences, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany $u Charité -Universitätsmedizin, Berlin, Germany $u DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, Berlin, Germany
700    1_
$a van Heesch, Sebastiaan $u Princess Máxima Center for Pediatric Oncology, Utrecht, Netherlands $1 https://orcid.org/0000000195931980
700    1_
$a Pravenec, Michal $u Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
700    1_
$a Atanur, Santosh S $u Department of Metabolism, Digestion, and Reproduction, Faculty of Medicine, Imperial College London, London, UK santosh.atanur@imperial.ac.uk $u The National Institute for Health Research, Imperial Biomedical Research Centre, ITMAT Data Science Group, Imperial College London, London, UK $1 https://orcid.org/0000000236699787
773    0_
$w MED00197314 $t Life science alliance $x 2575-1077 $g Roč. 5, č. 4 (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34996843 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20220506131449 $b ABA008
999    __
$a ok $b bmc $g 1789116 $s 1162570
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 5 $c 4 $e 20220107 $i 2575-1077 $m Life science alliance $n Life sci. alliance $x MED00197314
LZP    __
$a Pubmed-20220425
Back

Find record

Citation metrics

Archiving options