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Synthesis and biological evaluation of cationic TopFluor cholesterol analogues
M. Jurášek, J. Valečka, I. Novotný, Z. Kejík, J. Fähnrich, A. Marešová, J. Tauchen, P. Bartůněk, B. Dolenský, M. Jakubek, PB. Drašar, J. Králová
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- biologický transport MeSH
- buněčné linie MeSH
- cholesterol analogy a deriváty analýza chemická syntéza metabolismus MeSH
- lidé MeSH
- optické zobrazování MeSH
- sloučeniny boru analýza chemická syntéza chemie metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cholesterol is not only a major component of the cell membrane, but also plays an important role in a wide range of biological processes and pathologies. It is therefore crucial to develop appropriate tools for visualizing intracellular cholesterol transport. Here, we describe new cationic analogues of BODIPY-Cholesterol (TopFluor-Cholesterol, TF-Chol), which combine a positive charge on the sterol side chain and a BODIPY group connected via a C-4 linker. In contrast to TF-Chol, the new analogues TF-1 and TF-3 possessing acetyl groups on the A ring (C-3 position on steroid) internalized much faster and displayed slightly different levels of intracellular localization. Their applicability for cholesterol monitoring was indicated by the fact that they strongly label compartments with accumulated cholesterol in cells carrying a mutation of the Niemann-Pick disease-associated cholesterol transporter, NPC1.
Czech University of Life Sciences Prague Kamýcká 129 165 00 Prague 6 Czech Republic
University of Chemistry and Technology Technická 5 166 28 Prague 6 Czech Republic
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- $a Synthesis and biological evaluation of cationic TopFluor cholesterol analogues / $c M. Jurášek, J. Valečka, I. Novotný, Z. Kejík, J. Fähnrich, A. Marešová, J. Tauchen, P. Bartůněk, B. Dolenský, M. Jakubek, PB. Drašar, J. Králová
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- $a Cholesterol is not only a major component of the cell membrane, but also plays an important role in a wide range of biological processes and pathologies. It is therefore crucial to develop appropriate tools for visualizing intracellular cholesterol transport. Here, we describe new cationic analogues of BODIPY-Cholesterol (TopFluor-Cholesterol, TF-Chol), which combine a positive charge on the sterol side chain and a BODIPY group connected via a C-4 linker. In contrast to TF-Chol, the new analogues TF-1 and TF-3 possessing acetyl groups on the A ring (C-3 position on steroid) internalized much faster and displayed slightly different levels of intracellular localization. Their applicability for cholesterol monitoring was indicated by the fact that they strongly label compartments with accumulated cholesterol in cells carrying a mutation of the Niemann-Pick disease-associated cholesterol transporter, NPC1.
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