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Epitachophoresis is a novel versatile total nucleic acid extraction method
V. Datinska, P. Gheibi, K. Jefferson, J. Yang, S. Paladugu, C. Dallett, I. Voracova, F. Foret, Y. Astier
Language English Country Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
RVO 68081715
Institutional support of Institute of Analytical Chemistry
NLK
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- MeSH
- DNA, Neoplasm analysis chemistry isolation & purification MeSH
- Tissue Fixation MeSH
- Colorectal Neoplasms genetics pathology MeSH
- Humans MeSH
- Tumor Cells, Cultured MeSH
- Lung Neoplasms genetics pathology MeSH
- RNA, Neoplasm analysis chemistry isolation & purification MeSH
- High-Throughput Nucleotide Sequencing methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt-290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing.
References provided by Crossref.org
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