Epitachophoresis is a novel versatile total nucleic acid extraction method
Language English Country England, Great Britain Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
RVO 68081715
Institutional support of Institute of Analytical Chemistry
PubMed
34815497
PubMed Central
PMC8611068
DOI
10.1038/s41598-021-02214-1
PII: 10.1038/s41598-021-02214-1
Knihovny.cz E-resources
- MeSH
- DNA, Neoplasm analysis chemistry isolation & purification MeSH
- Tissue Fixation MeSH
- Colorectal Neoplasms genetics pathology MeSH
- Humans MeSH
- Tumor Cells, Cultured MeSH
- Lung Neoplasms genetics pathology MeSH
- RNA, Neoplasm analysis chemistry isolation & purification MeSH
- High-Throughput Nucleotide Sequencing methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Neoplasm MeSH
- RNA, Neoplasm MeSH
Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt-290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing.
See more in PubMed
Mullegama SV, et al. Nucleic acid extraction from human biological samples. Methods Mol. Biol. 2019;1897:359–383. doi: 10.1007/978-1-4939-8935-5_30. PubMed DOI
Thatcher SA. DNA/RNA preparation for molecular detection. Clin. Chem. 2015;61:89–99. doi: 10.1373/clinchem.2014.221374. PubMed DOI
Ali N, Rampazzo RDP, Costa ADT, Krieger MA. Current nucleic acid extraction methods and their implications to point-of-care diagnostics. Biomed. Res. Int. 2017;2017:1–13. PubMed PMC
Stellwagen NC. Electrophoresis of DNA in agarose gels, polyacrylamide gels and in free solution. Electrophoresis. 2009;30:S188–S195. doi: 10.1002/elps.200900052. PubMed DOI PMC
Kleparnik K, Bocek P. DNA diagnostics by capillary electrophoresis. Chem. Rev. 2007;107:5279–5317. doi: 10.1021/cr0101860. PubMed DOI
Foret F, et al. Macrofluidic device for preparative concentration based on epitachophoresis. Anal. Chem. 2019;91:7047–7053. doi: 10.1021/acs.analchem.8b05860. PubMed DOI
Rogacs A, Marshall LA, Santiago JG. Purification of nucleic acids using isotachophoresis. J. Chromatogr. A. 2014;1335:105–120. doi: 10.1016/j.chroma.2013.12.027. PubMed DOI
Datinska V, Voracova I, Schlecht U, Berka J, Foret F. Recent progress in nucleic acids isotachophoresis. J. Sep. Sci. 2018;41:236–247. doi: 10.1002/jssc.201700878. PubMed DOI
Voráčová I, et al. 3D printed device for epitachophoresis. Anal. Chim. Acta. 2021;1154:338246. doi: 10.1016/j.aca.2021.338246. PubMed DOI
Seiler C, et al. Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: A trade off between quantity and quality? BMC Clin. Pathol. 2016;16:17. doi: 10.1186/s12907-016-0039-3. PubMed DOI PMC
Nagahashi M, et al. Formalin-fixed paraffin-embedded sample conditions for deep next generation sequencing. J. Surg. Res. 2017;220:125–132. doi: 10.1016/j.jss.2017.06.077. PubMed DOI PMC
Chougule A, et al. Standardization, optimization, and quality management of FFPE solid tumor diagnostic samples in next-generation sequencing: An experience in our Tata Memorial Centre India. J. Mol. Diagn. 2019;21:1133–1134.
Jones W, et al. Deleterious effects of formalin-fixation and delays to fixation on RNA and miRNA-Seq profiles. Sci. Rep. UK. 2019;9:1–10. PubMed PMC
Chevillard S. A method for sequential extraction of Rna and DNA from the same sample, specially designed for a limited supply of biological-material. Biotechniques. 1993;15:22–000. PubMed
Wu KL, Tsai YM, Lien CT, Kuo PL, Hung JY. The roles of MicroRNA in lung cancer. Int. J. Mol. Sci. 2019;20:1611. doi: 10.3390/ijms20071611. PubMed DOI PMC
Wahid F, Shehzad A, Khan T, Kim YY. MicroRNAs: Synthesis, mechanism, function, and recent clinical trials. Bba-Mol. Cell Res. 2010;1803:1231–1243. PubMed
