Epitachophoresis is a novel versatile total nucleic acid extraction method
Jazyk angličtina Země Anglie, Velká Británie Médium electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
RVO 68081715
Institutional support of Institute of Analytical Chemistry
PubMed
34815497
PubMed Central
PMC8611068
DOI
10.1038/s41598-021-02214-1
PII: 10.1038/s41598-021-02214-1
Knihovny.cz E-zdroje
- MeSH
- DNA nádorová analýza chemie izolace a purifikace MeSH
- fixace tkání MeSH
- kolorektální nádory genetika patologie MeSH
- lidé MeSH
- nádorové buňky kultivované MeSH
- nádory plic genetika patologie MeSH
- RNA nádorová analýza chemie izolace a purifikace MeSH
- vysoce účinné nukleotidové sekvenování metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA nádorová MeSH
- RNA nádorová MeSH
Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt-290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing.
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