Recent progress in nucleic acids isotachophoresis
Language English Country Germany Media print-electronic
Document type Journal Article, Review
- Keywords
- isotachophoresis, nucleic acids, sample preparation,
- MeSH
- Automation MeSH
- DNA analysis MeSH
- Electrolytes MeSH
- Nucleic Acid Hybridization MeSH
- Isotachophoresis methods trends MeSH
- Humans MeSH
- MicroRNAs analysis MeSH
- Microfluidics MeSH
- Nucleic Acids isolation & purification MeSH
- Polymerase Chain Reaction MeSH
- Buffers MeSH
- Body Fluids metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Review MeSH
- Names of Substances
- DNA MeSH
- Electrolytes MeSH
- MicroRNAs MeSH
- Nucleic Acids MeSH
- Buffers MeSH
Progress achieved between 2014-2017 in the extraction and sample preparation of nucleic acid by isotachophoresis is reviewed in this paper. The isolation and purification of nucleic acids is very often compromised by a complex matrix such as blood and other bodily fluids, samples from the scene of crime, fossil samples, etc. While most of the common nucleic acids isolation techniques are based on extraction with inherent limitations with regard to quantitative results, isotachophoretic focusing is a quantitative process with a theoretically unlimited concentration factor. Since isotachophoresis belongs to less traditional approaches of nucleic acids purification, we present not only the latest developments in the application of isotachophoresis for the nucleic acids concentration but also a brief description of the principles of this method.
Czech Academy of Sciences Institute of Analytical Chemistry Brno Czech Republic
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